Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined Conditions
Non-human primates (NHPs) are pivotal animal models for translating novel cell replacement therapies into clinical applications, including validating the safety and efficacy of induced pluripotent stem cell (iPSC)-derived products. Preclinical development and the testing of cell-based therapies idea...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/13/6/558 |
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| author | Yuliia Tereshchenko Nesil Esiyok Enrique Garea-Rodríguez Daniele Repetto Rüdiger Behr Ignacio Rodríguez-Polo |
| author_facet | Yuliia Tereshchenko Nesil Esiyok Enrique Garea-Rodríguez Daniele Repetto Rüdiger Behr Ignacio Rodríguez-Polo |
| author_sort | Yuliia Tereshchenko |
| collection | DOAJ |
| description | Non-human primates (NHPs) are pivotal animal models for translating novel cell replacement therapies into clinical applications, including validating the safety and efficacy of induced pluripotent stem cell (iPSC)-derived products. Preclinical development and the testing of cell-based therapies ideally comprise xenogeneic (human stem cells into NHPs) and allogenic (NHP stem cells into NHPs) transplantation studies. For the allogeneic approach, it is necessary to generate NHP-iPSCs with generally equivalent quality to the human counterparts that will be used later on in patients. Here, we report the generation and characterization of transgene- and feeder-free cynomolgus monkey (<i>Macaca fascicularis</i>) iPSCs (Cyno-iPSCs). These novel cell lines have been generated according to a previously developed protocol for the generation of rhesus macaque, baboon, and human iPSC lines. Beyond their generation, we demonstrate the potential of the novel Cyno-iPSCs to differentiate into two clinically relevant cell types, i.e., cardiomyocytes and neurons. Overall, we provide a resource of novel iPSCs from the most frequently used NHP species in the regulatory testing of biologics and classical pharmaceutics to expand our panel of iPSC lines from NHP species with high relevance in preclinical testing and translational research. |
| first_indexed | 2024-04-24T18:26:38Z |
| format | Article |
| id | doaj.art-3a80dc75f3944dcb8776d95e08dd56b1 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-04-24T18:26:38Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-3a80dc75f3944dcb8776d95e08dd56b12024-03-27T13:30:45ZengMDPI AGCells2073-44092024-03-0113655810.3390/cells13060558Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined ConditionsYuliia Tereshchenko0Nesil Esiyok1Enrique Garea-Rodríguez2Daniele Repetto3Rüdiger Behr4Ignacio Rodríguez-Polo5Research Platform Degenerative Diseases, German Primate Center-Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, GermanyResearch Platform Degenerative Diseases, German Primate Center-Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, GermanyCharles River Laboratories Germany GmbH, Hans-Adolf-Krebs-Weg 9, 37077 Göttingen, GermanyCharles River Laboratories Germany GmbH, Hans-Adolf-Krebs-Weg 9, 37077 Göttingen, GermanyResearch Platform Degenerative Diseases, German Primate Center-Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, GermanyResearch Platform Degenerative Diseases, German Primate Center-Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, GermanyNon-human primates (NHPs) are pivotal animal models for translating novel cell replacement therapies into clinical applications, including validating the safety and efficacy of induced pluripotent stem cell (iPSC)-derived products. Preclinical development and the testing of cell-based therapies ideally comprise xenogeneic (human stem cells into NHPs) and allogenic (NHP stem cells into NHPs) transplantation studies. For the allogeneic approach, it is necessary to generate NHP-iPSCs with generally equivalent quality to the human counterparts that will be used later on in patients. Here, we report the generation and characterization of transgene- and feeder-free cynomolgus monkey (<i>Macaca fascicularis</i>) iPSCs (Cyno-iPSCs). These novel cell lines have been generated according to a previously developed protocol for the generation of rhesus macaque, baboon, and human iPSC lines. Beyond their generation, we demonstrate the potential of the novel Cyno-iPSCs to differentiate into two clinically relevant cell types, i.e., cardiomyocytes and neurons. Overall, we provide a resource of novel iPSCs from the most frequently used NHP species in the regulatory testing of biologics and classical pharmaceutics to expand our panel of iPSC lines from NHP species with high relevance in preclinical testing and translational research.https://www.mdpi.com/2073-4409/13/6/558macaqueiPSCstem cellnon-human primateneuronal differentiationcardiac differentiation |
| spellingShingle | Yuliia Tereshchenko Nesil Esiyok Enrique Garea-Rodríguez Daniele Repetto Rüdiger Behr Ignacio Rodríguez-Polo Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined Conditions Cells macaque iPSC stem cell non-human primate neuronal differentiation cardiac differentiation |
| title | Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined Conditions |
| title_full | Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined Conditions |
| title_fullStr | Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined Conditions |
| title_full_unstemmed | Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined Conditions |
| title_short | Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined Conditions |
| title_sort | transgene free cynomolgus monkey ipscs generated under chemically defined conditions |
| topic | macaque iPSC stem cell non-human primate neuronal differentiation cardiac differentiation |
| url | https://www.mdpi.com/2073-4409/13/6/558 |
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