Transcriptional profiles of hybrid Eucalyptus genotypes with contrasting lignin content reveal that monolignol biosynthesis-related genes regulate wood composition
Eucalyptus species constitutes the most widely planted hardwood trees in temperate and subtropical regions. In this study, we compared the transcript levels of genes involved in lignocellulose formation such as cellulose, hemicellulose and lignin biosynthesis in two selected three-year old hybrid Eu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00443/full |
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| author | Tomotaka eShinya Eiji eIwata Katsuhiko eNakahama Yujiroh eFukuda Kazunori eHayashi Kazuya eNanto Antonio C. Rosa Akiyoshi eKawaoka |
| author_facet | Tomotaka eShinya Eiji eIwata Katsuhiko eNakahama Yujiroh eFukuda Kazunori eHayashi Kazuya eNanto Antonio C. Rosa Akiyoshi eKawaoka |
| author_sort | Tomotaka eShinya |
| collection | DOAJ |
| description | Eucalyptus species constitutes the most widely planted hardwood trees in temperate and subtropical regions. In this study, we compared the transcript levels of genes involved in lignocellulose formation such as cellulose, hemicellulose and lignin biosynthesis in two selected three-year old hybrid Eucalyptus (Eucalyptus urophylla x E. grandis) genotypes (AM063 and AM380) that have different lignin content. AM063 and AM380 had 20.2 and 35.5% of Klason lignin content and 59.0% and 48.2%, -cellulose contents, respectively. We investigated the correlation between wood properties and transcript levels of wood formation-related genes using RNA-seq with total RNAs extracted from developing xylem tissues at a breast height. Transcript levels of cell wall construction genes such as cellulose synthase (CesA) and sucrose synthase (SUSY) were almost the same in both genotypes. However, AM063 exhibited higher transcript levels of UDP-glucose pyrophosphorylase (UGP) and xyloglucan endotransglucoxylase (XTH) than those in AM380. Most monolignol biosynthesis- related isozyme genes showed higher transcript levels in AM380. These results indicate monolignol biosynthesis-related genes may regulate wood composition in Eucalyptus. Flavonoids contents were also observed at much higher levels in AM380 as a result of the elevated transcript levels of common phenylpropanoid pathway genes, phenylalanine ammonium lyase (PAL), cinnamate-4-hydroxylase (C4H) and 4-coumarate-CoA ligase (4CL). Secondary plant cell wall formation is regulated by many transcription factors. We analyzed genes encoding NAC, WRKY, AP2/ERF and KNOX transcription factors and found higher transcript levels of these genes in AM380. We also observed increased transcription of some MYB and LIM domain transcription factors in AM380 compared to AM063. All these results show that genes related to monolignol biosynthesis may regulate the wood composition and help maintain the ratio of cellulose and lignin contents in Eucalyptus plants. |
| first_indexed | 2024-12-12T15:50:47Z |
| format | Article |
| id | doaj.art-3a81bc410d364291832cc1bdde53371a |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T15:50:47Z |
| publishDate | 2016-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-3a81bc410d364291832cc1bdde53371a2022-12-22T00:19:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-04-01710.3389/fpls.2016.00443175199Transcriptional profiles of hybrid Eucalyptus genotypes with contrasting lignin content reveal that monolignol biosynthesis-related genes regulate wood compositionTomotaka eShinya0Eiji eIwata1Katsuhiko eNakahama2Yujiroh eFukuda3Kazunori eHayashi4Kazuya eNanto5Antonio C. Rosa6Akiyoshi eKawaoka7Nippon Paper Industries Co.,ltd.Nippon Paper Industries Co.,ltd.Nippon Paper Industries Co.,ltd.Nippon Paper Industries Co.,ltd.Amapa Frorestal e Celulose S.A.Nippon Paper Industries Co.,ltd.Amapa Frorestal e Celulose S.A.Nippon Paper Industries Co.,ltd.Eucalyptus species constitutes the most widely planted hardwood trees in temperate and subtropical regions. In this study, we compared the transcript levels of genes involved in lignocellulose formation such as cellulose, hemicellulose and lignin biosynthesis in two selected three-year old hybrid Eucalyptus (Eucalyptus urophylla x E. grandis) genotypes (AM063 and AM380) that have different lignin content. AM063 and AM380 had 20.2 and 35.5% of Klason lignin content and 59.0% and 48.2%, -cellulose contents, respectively. We investigated the correlation between wood properties and transcript levels of wood formation-related genes using RNA-seq with total RNAs extracted from developing xylem tissues at a breast height. Transcript levels of cell wall construction genes such as cellulose synthase (CesA) and sucrose synthase (SUSY) were almost the same in both genotypes. However, AM063 exhibited higher transcript levels of UDP-glucose pyrophosphorylase (UGP) and xyloglucan endotransglucoxylase (XTH) than those in AM380. Most monolignol biosynthesis- related isozyme genes showed higher transcript levels in AM380. These results indicate monolignol biosynthesis-related genes may regulate wood composition in Eucalyptus. Flavonoids contents were also observed at much higher levels in AM380 as a result of the elevated transcript levels of common phenylpropanoid pathway genes, phenylalanine ammonium lyase (PAL), cinnamate-4-hydroxylase (C4H) and 4-coumarate-CoA ligase (4CL). Secondary plant cell wall formation is regulated by many transcription factors. We analyzed genes encoding NAC, WRKY, AP2/ERF and KNOX transcription factors and found higher transcript levels of these genes in AM380. We also observed increased transcription of some MYB and LIM domain transcription factors in AM380 compared to AM063. All these results show that genes related to monolignol biosynthesis may regulate the wood composition and help maintain the ratio of cellulose and lignin contents in Eucalyptus plants.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00443/fullCelluloseLignintranscription factorRNA-SeqFlavonoidHybrid Eucalyptus |
| spellingShingle | Tomotaka eShinya Eiji eIwata Katsuhiko eNakahama Yujiroh eFukuda Kazunori eHayashi Kazuya eNanto Antonio C. Rosa Akiyoshi eKawaoka Transcriptional profiles of hybrid Eucalyptus genotypes with contrasting lignin content reveal that monolignol biosynthesis-related genes regulate wood composition Frontiers in Plant Science Cellulose Lignin transcription factor RNA-Seq Flavonoid Hybrid Eucalyptus |
| title | Transcriptional profiles of hybrid Eucalyptus genotypes with contrasting lignin content reveal that monolignol biosynthesis-related genes regulate wood composition |
| title_full | Transcriptional profiles of hybrid Eucalyptus genotypes with contrasting lignin content reveal that monolignol biosynthesis-related genes regulate wood composition |
| title_fullStr | Transcriptional profiles of hybrid Eucalyptus genotypes with contrasting lignin content reveal that monolignol biosynthesis-related genes regulate wood composition |
| title_full_unstemmed | Transcriptional profiles of hybrid Eucalyptus genotypes with contrasting lignin content reveal that monolignol biosynthesis-related genes regulate wood composition |
| title_short | Transcriptional profiles of hybrid Eucalyptus genotypes with contrasting lignin content reveal that monolignol biosynthesis-related genes regulate wood composition |
| title_sort | transcriptional profiles of hybrid eucalyptus genotypes with contrasting lignin content reveal that monolignol biosynthesis related genes regulate wood composition |
| topic | Cellulose Lignin transcription factor RNA-Seq Flavonoid Hybrid Eucalyptus |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00443/full |
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