Conservation of noIR in the Sinorhizobium and Rhizobium Genera of the Rhizobiaceae Family
In Sinorhizobium meliloti the NolR repressor displays differential negative regulation of nodulation genes and is required for optimal nodulation. Here, we demonstrate that the NolR function is not unique to S. meliloti but is also present in other species of the Rhizobiaceae family. DNA hybridizati...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
1998-12-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.12.1186 |
| _version_ | 1818757328219930624 |
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| author | Ernö Kiss Peter Mergaert Boglàrka Olàh Attila Kereszt Christian Staehelin Andrea E. Davies J. Allan Downie Adam Kondorosi Eva Kondorosi |
| author_facet | Ernö Kiss Peter Mergaert Boglàrka Olàh Attila Kereszt Christian Staehelin Andrea E. Davies J. Allan Downie Adam Kondorosi Eva Kondorosi |
| author_sort | Ernö Kiss |
| collection | DOAJ |
| description | In Sinorhizobium meliloti the NolR repressor displays differential negative regulation of nodulation genes and is required for optimal nodulation. Here, we demonstrate that the NolR function is not unique to S. meliloti but is also present in other species of the Rhizobiaceae family. DNA hybridization indicates the presence of nolR homologous sequences in species belonging to the Rhizobium and Sinorhizobium genera while no hybridization signal was detected in species from the Mesorhizobium, Bradyrhizo-bium, Azorhizobium, and Agrobacterium genera. We isolated the nolR gene from the Rhizobium leguminosarum bv. viciae strain TOM and showed that the TOM nolR gene acts similarly to S. meliloti nolR by repressing the expression of both the nodABCIJ and the nodD genes, resulting in decreased Nod factor production. The presence of a functional nolR gene in R. leguminosarum is correlated with an increased rate and extent of nodulation of pea. The conserved primary structure, the location of the DNA-binding domain, and the similar size of NolR proteins, compared with a family of small bacterial regulatory proteins including HlyU, SmtB, and the ArsR-type regulators, revealed that NolR belongs to this family. |
| first_indexed | 2024-12-18T06:09:11Z |
| format | Article |
| id | doaj.art-3a824ef58e9644e8ae222277b5f083b8 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-18T06:09:11Z |
| publishDate | 1998-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-3a824ef58e9644e8ae222277b5f083b82022-12-21T21:18:27ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061998-12-0111121186119510.1094/MPMI.1998.11.12.1186Conservation of noIR in the Sinorhizobium and Rhizobium Genera of the Rhizobiaceae FamilyErnö KissPeter MergaertBoglàrka OlàhAttila KeresztChristian StaehelinAndrea E. DaviesJ. Allan DownieAdam KondorosiEva KondorosiIn Sinorhizobium meliloti the NolR repressor displays differential negative regulation of nodulation genes and is required for optimal nodulation. Here, we demonstrate that the NolR function is not unique to S. meliloti but is also present in other species of the Rhizobiaceae family. DNA hybridization indicates the presence of nolR homologous sequences in species belonging to the Rhizobium and Sinorhizobium genera while no hybridization signal was detected in species from the Mesorhizobium, Bradyrhizo-bium, Azorhizobium, and Agrobacterium genera. We isolated the nolR gene from the Rhizobium leguminosarum bv. viciae strain TOM and showed that the TOM nolR gene acts similarly to S. meliloti nolR by repressing the expression of both the nodABCIJ and the nodD genes, resulting in decreased Nod factor production. The presence of a functional nolR gene in R. leguminosarum is correlated with an increased rate and extent of nodulation of pea. The conserved primary structure, the location of the DNA-binding domain, and the similar size of NolR proteins, compared with a family of small bacterial regulatory proteins including HlyU, SmtB, and the ArsR-type regulators, revealed that NolR belongs to this family.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.12.1186 |
| spellingShingle | Ernö Kiss Peter Mergaert Boglàrka Olàh Attila Kereszt Christian Staehelin Andrea E. Davies J. Allan Downie Adam Kondorosi Eva Kondorosi Conservation of noIR in the Sinorhizobium and Rhizobium Genera of the Rhizobiaceae Family Molecular Plant-Microbe Interactions |
| title | Conservation of noIR in the Sinorhizobium and Rhizobium Genera of the Rhizobiaceae Family |
| title_full | Conservation of noIR in the Sinorhizobium and Rhizobium Genera of the Rhizobiaceae Family |
| title_fullStr | Conservation of noIR in the Sinorhizobium and Rhizobium Genera of the Rhizobiaceae Family |
| title_full_unstemmed | Conservation of noIR in the Sinorhizobium and Rhizobium Genera of the Rhizobiaceae Family |
| title_short | Conservation of noIR in the Sinorhizobium and Rhizobium Genera of the Rhizobiaceae Family |
| title_sort | conservation of noir in the sinorhizobium and rhizobium genera of the rhizobiaceae family |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1998.11.12.1186 |
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