Isolation and Identification of Spoilage Yeasts in Wine Samples by MALDI-TOF MS Biotyper
Many genera and species of microorganisms can be found in grape musts and wines at various times during the winemaking process. For instance, Saccharomyces, Brettanomyces, and Pediococcus can be found together in wine. There are many species of yeast involved in wine spoilage during storage. Aim of...
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Format: | Article |
Language: | English |
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Agroprint Timisoara
2023-09-01
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Series: | Scientific Papers Animal Science and Biotechnologies |
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Online Access: | https://spasb.ro/index.php/public_html/article/view/239 |
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author | Attila Kántor Miroslava Kačániová |
author_facet | Attila Kántor Miroslava Kačániová |
author_sort | Attila Kántor |
collection | DOAJ |
description | Many genera and species of microorganisms can be found in grape musts and wines at various times during the winemaking process. For instance, Saccharomyces, Brettanomyces, and Pediococcus can be found together in wine. There are many species of yeast involved in wine spoilage during storage. Aim of this study was to isolate the spoilage yeasts from wine samples with using special selective agar media and identified on species level by Matrix-Assisted Laser Desorption/Ionization-Time of Fly Mass Spectrometry (MALDI-TOF MS). Six red wines used in this study. We identified 10 yeast species from 152 isolates. The most common species in wine samples was Saccharomyces cerevisiae. We also identified four Candida species, two Zygosaccharomyces species and one species from genus Rhodotorula, Saccharomycodes and Dekkera. |
first_indexed | 2024-04-24T11:19:01Z |
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institution | Directory Open Access Journal |
issn | 1841-9364 2344-4576 |
language | English |
last_indexed | 2024-04-24T11:19:01Z |
publishDate | 2023-09-01 |
publisher | Agroprint Timisoara |
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series | Scientific Papers Animal Science and Biotechnologies |
spelling | doaj.art-3a83752df11440ebbd02bb2f7aaeafa12024-04-11T05:01:33ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-01481156156239Isolation and Identification of Spoilage Yeasts in Wine Samples by MALDI-TOF MS BiotyperAttila Kántor0Miroslava Kačániová1Slovak University of Agriculture in Nitra, 949 76, Tr. A. Hlinku 2, NitraSlovak University of Agriculture in Nitra, 949 76, Tr. A. Hlinku 2, NitraMany genera and species of microorganisms can be found in grape musts and wines at various times during the winemaking process. For instance, Saccharomyces, Brettanomyces, and Pediococcus can be found together in wine. There are many species of yeast involved in wine spoilage during storage. Aim of this study was to isolate the spoilage yeasts from wine samples with using special selective agar media and identified on species level by Matrix-Assisted Laser Desorption/Ionization-Time of Fly Mass Spectrometry (MALDI-TOF MS). Six red wines used in this study. We identified 10 yeast species from 152 isolates. The most common species in wine samples was Saccharomyces cerevisiae. We also identified four Candida species, two Zygosaccharomyces species and one species from genus Rhodotorula, Saccharomycodes and Dekkera.https://spasb.ro/index.php/public_html/article/view/239wineyeastsmaldi-tof ms biotyper |
spellingShingle | Attila Kántor Miroslava Kačániová Isolation and Identification of Spoilage Yeasts in Wine Samples by MALDI-TOF MS Biotyper Scientific Papers Animal Science and Biotechnologies wine yeasts maldi-tof ms biotyper |
title | Isolation and Identification of Spoilage Yeasts in Wine Samples by MALDI-TOF MS Biotyper |
title_full | Isolation and Identification of Spoilage Yeasts in Wine Samples by MALDI-TOF MS Biotyper |
title_fullStr | Isolation and Identification of Spoilage Yeasts in Wine Samples by MALDI-TOF MS Biotyper |
title_full_unstemmed | Isolation and Identification of Spoilage Yeasts in Wine Samples by MALDI-TOF MS Biotyper |
title_short | Isolation and Identification of Spoilage Yeasts in Wine Samples by MALDI-TOF MS Biotyper |
title_sort | isolation and identification of spoilage yeasts in wine samples by maldi tof ms biotyper |
topic | wine yeasts maldi-tof ms biotyper |
url | https://spasb.ro/index.php/public_html/article/view/239 |
work_keys_str_mv | AT attilakantor isolationandidentificationofspoilageyeastsinwinesamplesbymalditofmsbiotyper AT miroslavakacaniova isolationandidentificationofspoilageyeastsinwinesamplesbymalditofmsbiotyper |