Isolation and Identification of Spoilage Yeasts in Wine Samples by MALDI-TOF MS Biotyper

Many genera and species of microorganisms can be found in grape musts and wines at various times during the winemaking process. For instance, Saccharomyces, Brettanomyces, and Pediococcus can be found together in wine. There are many species of yeast involved in wine spoilage during storage. Aim of...

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Main Authors: Attila Kántor, Miroslava Kačániová
Format: Article
Language:English
Published: Agroprint Timisoara 2023-09-01
Series:Scientific Papers Animal Science and Biotechnologies
Subjects:
Online Access:https://spasb.ro/index.php/public_html/article/view/239
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author Attila Kántor
Miroslava Kačániová
author_facet Attila Kántor
Miroslava Kačániová
author_sort Attila Kántor
collection DOAJ
description Many genera and species of microorganisms can be found in grape musts and wines at various times during the winemaking process. For instance, Saccharomyces, Brettanomyces, and Pediococcus can be found together in wine. There are many species of yeast involved in wine spoilage during storage. Aim of this study was to isolate the spoilage yeasts from wine samples with using special selective agar media and identified on species level by Matrix-Assisted Laser Desorption/Ionization-Time of Fly Mass Spectrometry (MALDI-TOF MS). Six red wines used in this study. We identified 10 yeast species from 152 isolates. The most common species in wine samples was Saccharomyces cerevisiae. We also identified four Candida species, two Zygosaccharomyces species and one species from genus Rhodotorula, Saccharomycodes and Dekkera.
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spelling doaj.art-3a83752df11440ebbd02bb2f7aaeafa12024-04-11T05:01:33ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-01481156156239Isolation and Identification of Spoilage Yeasts in Wine Samples by MALDI-TOF MS BiotyperAttila Kántor0Miroslava Kačániová1Slovak University of Agriculture in Nitra, 949 76, Tr. A. Hlinku 2, NitraSlovak University of Agriculture in Nitra, 949 76, Tr. A. Hlinku 2, NitraMany genera and species of microorganisms can be found in grape musts and wines at various times during the winemaking process. For instance, Saccharomyces, Brettanomyces, and Pediococcus can be found together in wine. There are many species of yeast involved in wine spoilage during storage. Aim of this study was to isolate the spoilage yeasts from wine samples with using special selective agar media and identified on species level by Matrix-Assisted Laser Desorption/Ionization-Time of Fly Mass Spectrometry (MALDI-TOF MS). Six red wines used in this study. We identified 10 yeast species from 152 isolates. The most common species in wine samples was Saccharomyces cerevisiae. We also identified four Candida species, two Zygosaccharomyces species and one species from genus Rhodotorula, Saccharomycodes and Dekkera.https://spasb.ro/index.php/public_html/article/view/239wineyeastsmaldi-tof ms biotyper
spellingShingle Attila Kántor
Miroslava Kačániová
Isolation and Identification of Spoilage Yeasts in Wine Samples by MALDI-TOF MS Biotyper
Scientific Papers Animal Science and Biotechnologies
wine
yeasts
maldi-tof ms biotyper
title Isolation and Identification of Spoilage Yeasts in Wine Samples by MALDI-TOF MS Biotyper
title_full Isolation and Identification of Spoilage Yeasts in Wine Samples by MALDI-TOF MS Biotyper
title_fullStr Isolation and Identification of Spoilage Yeasts in Wine Samples by MALDI-TOF MS Biotyper
title_full_unstemmed Isolation and Identification of Spoilage Yeasts in Wine Samples by MALDI-TOF MS Biotyper
title_short Isolation and Identification of Spoilage Yeasts in Wine Samples by MALDI-TOF MS Biotyper
title_sort isolation and identification of spoilage yeasts in wine samples by maldi tof ms biotyper
topic wine
yeasts
maldi-tof ms biotyper
url https://spasb.ro/index.php/public_html/article/view/239
work_keys_str_mv AT attilakantor isolationandidentificationofspoilageyeastsinwinesamplesbymalditofmsbiotyper
AT miroslavakacaniova isolationandidentificationofspoilageyeastsinwinesamplesbymalditofmsbiotyper