Dietary effect of low fish meal aquafeed on gut microbiota in olive flounder (Paralichthys olivaceus) at different growth stages
Abstract This study was conducted to investigate the long‐term effect of a low fish meal (FM) diet comprising plant‐based protein sources (PPS) on changes of gut microbial diversity in olive flounder (Paralichthys olivaceus) over the course of life. Two experimental diets were prepared to contain 74...
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| Format: | Article |
| Language: | English |
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Wiley
2020-03-01
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| Series: | MicrobiologyOpen |
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| Online Access: | https://doi.org/10.1002/mbo3.992 |
| _version_ | 1818417766894403584 |
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| author | Kai‐Min Niu Bong‐Joo Lee Damini Kothari Woo‐Do Lee Sang‐Woo Hur Sang‐Gu Lim Kang‐Woong Kim Kyoung‐Duck Kim Na‐Na Kim Soo‐Ki Kim |
| author_facet | Kai‐Min Niu Bong‐Joo Lee Damini Kothari Woo‐Do Lee Sang‐Woo Hur Sang‐Gu Lim Kang‐Woong Kim Kyoung‐Duck Kim Na‐Na Kim Soo‐Ki Kim |
| author_sort | Kai‐Min Niu |
| collection | DOAJ |
| description | Abstract This study was conducted to investigate the long‐term effect of a low fish meal (FM) diet comprising plant‐based protein sources (PPS) on changes of gut microbial diversity in olive flounder (Paralichthys olivaceus) over the course of life. Two experimental diets were prepared to contain 74% FM (control) or 52% FM with 22% PPS (30% FM replacement, FM30). Fish were fed one of the two experimental diets for 8 months, and we collected the midgut contents to analyze the gut bacterial community by Illumina MiSeq based on the metagenomic sequences in the V3–V4 regions of 16S rRNA. We found that there were nine dominant phyla, which in turn presented Proteobacteria, Firmicutes, and Actinobacteria as the three major phyla in the gut microbiota of the flounder. At genus level, the dominant genera were Delftia, Prevotella, and Chthoniobacter at the juvenile stage (below 100 g/fish); Chthoniobacter, Bacillus, and Bradyrhizobium at the grower stage (400 g/fish); Chthoniobacter, Bacillus, and Delftia at the subadult stage (800 g/fish); and Lactobacillus and Prevotella at the adult stage (over 1,000 g/fish). The microbial diversity in olive flounders arched from the juvenile and subadult stage and reached a plateau thereafter. The fish fed the FM30 diet significantly had an increased abundance of Lactobacillus and Photobacterium and had less abundance of Prevotella and Paraprevotella than the control. However, the effect of dietary PPS was not significant on total microbial richness, indicating no negative effect as feed sources on the intestinal microbiota in olive flounder. These results indicate that the life stage of olive flounder is more important in modulating intestinal microbiota than is the diet. It could also be concluded that dietary PPS might be used as a potential fish meal alternative without any compromising effects on microbial diversity of olive flounder for long‐term feeding. |
| first_indexed | 2024-12-14T12:12:00Z |
| format | Article |
| id | doaj.art-3a83c24bbbf045c9b94cbef45ccab3ed |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-12-14T12:12:00Z |
| publishDate | 2020-03-01 |
| publisher | Wiley |
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| series | MicrobiologyOpen |
| spelling | doaj.art-3a83c24bbbf045c9b94cbef45ccab3ed2022-12-21T23:01:42ZengWileyMicrobiologyOpen2045-88272020-03-0193n/an/a10.1002/mbo3.992Dietary effect of low fish meal aquafeed on gut microbiota in olive flounder (Paralichthys olivaceus) at different growth stagesKai‐Min Niu0Bong‐Joo Lee1Damini Kothari2Woo‐Do Lee3Sang‐Woo Hur4Sang‐Gu Lim5Kang‐Woong Kim6Kyoung‐Duck Kim7Na‐Na Kim8Soo‐Ki Kim9Institute of Biological Resources Jiangxi Academy of Sciences Nanchang ChinaAquafeed Research Center National Institute of Fisheries Science Pohang KoreaDepartment of Animal Science and Technology Konkuk University Seoul KoreaDepartment of Animal Science and Technology Konkuk University Seoul KoreaAquafeed Research Center National Institute of Fisheries Science Pohang KoreaAquafeed Research Center National Institute of Fisheries Science Pohang KoreaAquaculture Management Division National Institute of Fisheries Science Busan KoreaAquaculture Management Division National Institute of Fisheries Science Busan KoreaInland Aquaculture Research Center National Institute of Fisheries Science Changwon KoreaDepartment of Animal Science and Technology Konkuk University Seoul KoreaAbstract This study was conducted to investigate the long‐term effect of a low fish meal (FM) diet comprising plant‐based protein sources (PPS) on changes of gut microbial diversity in olive flounder (Paralichthys olivaceus) over the course of life. Two experimental diets were prepared to contain 74% FM (control) or 52% FM with 22% PPS (30% FM replacement, FM30). Fish were fed one of the two experimental diets for 8 months, and we collected the midgut contents to analyze the gut bacterial community by Illumina MiSeq based on the metagenomic sequences in the V3–V4 regions of 16S rRNA. We found that there were nine dominant phyla, which in turn presented Proteobacteria, Firmicutes, and Actinobacteria as the three major phyla in the gut microbiota of the flounder. At genus level, the dominant genera were Delftia, Prevotella, and Chthoniobacter at the juvenile stage (below 100 g/fish); Chthoniobacter, Bacillus, and Bradyrhizobium at the grower stage (400 g/fish); Chthoniobacter, Bacillus, and Delftia at the subadult stage (800 g/fish); and Lactobacillus and Prevotella at the adult stage (over 1,000 g/fish). The microbial diversity in olive flounders arched from the juvenile and subadult stage and reached a plateau thereafter. The fish fed the FM30 diet significantly had an increased abundance of Lactobacillus and Photobacterium and had less abundance of Prevotella and Paraprevotella than the control. However, the effect of dietary PPS was not significant on total microbial richness, indicating no negative effect as feed sources on the intestinal microbiota in olive flounder. These results indicate that the life stage of olive flounder is more important in modulating intestinal microbiota than is the diet. It could also be concluded that dietary PPS might be used as a potential fish meal alternative without any compromising effects on microbial diversity of olive flounder for long‐term feeding.https://doi.org/10.1002/mbo3.992growth stagegut microbiotalow fish mealnext‐generation sequencingolive flounder |
| spellingShingle | Kai‐Min Niu Bong‐Joo Lee Damini Kothari Woo‐Do Lee Sang‐Woo Hur Sang‐Gu Lim Kang‐Woong Kim Kyoung‐Duck Kim Na‐Na Kim Soo‐Ki Kim Dietary effect of low fish meal aquafeed on gut microbiota in olive flounder (Paralichthys olivaceus) at different growth stages MicrobiologyOpen growth stage gut microbiota low fish meal next‐generation sequencing olive flounder |
| title | Dietary effect of low fish meal aquafeed on gut microbiota in olive flounder (Paralichthys olivaceus) at different growth stages |
| title_full | Dietary effect of low fish meal aquafeed on gut microbiota in olive flounder (Paralichthys olivaceus) at different growth stages |
| title_fullStr | Dietary effect of low fish meal aquafeed on gut microbiota in olive flounder (Paralichthys olivaceus) at different growth stages |
| title_full_unstemmed | Dietary effect of low fish meal aquafeed on gut microbiota in olive flounder (Paralichthys olivaceus) at different growth stages |
| title_short | Dietary effect of low fish meal aquafeed on gut microbiota in olive flounder (Paralichthys olivaceus) at different growth stages |
| title_sort | dietary effect of low fish meal aquafeed on gut microbiota in olive flounder paralichthys olivaceus at different growth stages |
| topic | growth stage gut microbiota low fish meal next‐generation sequencing olive flounder |
| url | https://doi.org/10.1002/mbo3.992 |
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