<i>miR-200b</i>, <i>ZEB2</i> and <i>PTPN13</i> Are Downregulated in Colorectal Carcinoma with Serosal Invasion
Serosal invasion is an independent negative prognostic factor in certain cancers, including CRC. However, the mechanisms behind serosal invasion are poorly understood. We therefore assumed that epithelial-mesenchymal transition (EMT) might be involved. Our study included 34 patients with CRC, 3 stag...
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MDPI AG
2022-09-01
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| Online Access: | https://www.mdpi.com/2227-9059/10/9/2149 |
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| author | Branislava Ranković Emanuela Boštjančič Nina Zidar Margareta Žlajpah Jera Jeruc |
| author_facet | Branislava Ranković Emanuela Boštjančič Nina Zidar Margareta Žlajpah Jera Jeruc |
| author_sort | Branislava Ranković |
| collection | DOAJ |
| description | Serosal invasion is an independent negative prognostic factor in certain cancers, including CRC. However, the mechanisms behind serosal invasion are poorly understood. We therefore assumed that epithelial-mesenchymal transition (EMT) might be involved. Our study included 34 patients with CRC, 3 stage pT2, 14 stage pT3 and 17 showing serosal invasion (stage pT4a according to TNM staging system). RNA isolated from formalin-fixed paraffin-embedded tissue samples was analysed for expression of the <i>miR-200</i> family and their target genes <i>CDKN1B, ONECUT2, PTPN13, RND3, SOX2, TGFB2</i> and <i>ZEB2</i> using real-time PCR. We found upregulation of <i>miR-200b</i> and <i>ONECUT2</i> in CRC pT3 and pT4a compared to normal mucosa, and downregulation of <i>CDKN1B</i> in CRC pT3. Moreover, we observed, downregulation of <i>miR-200b, PTPN13</i> and <i>ZEB2</i> in CRC with serosal invasion (pT4a) compared to pT3. Our results suggest the involvement of partial EMT in serosal invasion of CRC. In addition, <i>PTPN13</i> seems to be one of the important regulators involved in serosal invasion, and <i>ONECUT2</i> in tumour growth. |
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| language | English |
| last_indexed | 2024-03-10T00:39:05Z |
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| spelling | doaj.art-3a85e5f4f9ae493d831e2f9c443bca382023-11-23T15:09:55ZengMDPI AGBiomedicines2227-90592022-09-01109214910.3390/biomedicines10092149<i>miR-200b</i>, <i>ZEB2</i> and <i>PTPN13</i> Are Downregulated in Colorectal Carcinoma with Serosal InvasionBranislava Ranković0Emanuela Boštjančič1Nina Zidar2Margareta Žlajpah3Jera Jeruc4Faculty of Medicine, Institute of Pathology, University of Ljubljana, Korytkova 2, 1000 Ljubljana, SloveniaFaculty of Medicine, Institute of Pathology, University of Ljubljana, Korytkova 2, 1000 Ljubljana, SloveniaFaculty of Medicine, Institute of Pathology, University of Ljubljana, Korytkova 2, 1000 Ljubljana, SloveniaFaculty of Medicine, Institute of Pathology, University of Ljubljana, Korytkova 2, 1000 Ljubljana, SloveniaFaculty of Medicine, Institute of Pathology, University of Ljubljana, Korytkova 2, 1000 Ljubljana, SloveniaSerosal invasion is an independent negative prognostic factor in certain cancers, including CRC. However, the mechanisms behind serosal invasion are poorly understood. We therefore assumed that epithelial-mesenchymal transition (EMT) might be involved. Our study included 34 patients with CRC, 3 stage pT2, 14 stage pT3 and 17 showing serosal invasion (stage pT4a according to TNM staging system). RNA isolated from formalin-fixed paraffin-embedded tissue samples was analysed for expression of the <i>miR-200</i> family and their target genes <i>CDKN1B, ONECUT2, PTPN13, RND3, SOX2, TGFB2</i> and <i>ZEB2</i> using real-time PCR. We found upregulation of <i>miR-200b</i> and <i>ONECUT2</i> in CRC pT3 and pT4a compared to normal mucosa, and downregulation of <i>CDKN1B</i> in CRC pT3. Moreover, we observed, downregulation of <i>miR-200b, PTPN13</i> and <i>ZEB2</i> in CRC with serosal invasion (pT4a) compared to pT3. Our results suggest the involvement of partial EMT in serosal invasion of CRC. In addition, <i>PTPN13</i> seems to be one of the important regulators involved in serosal invasion, and <i>ONECUT2</i> in tumour growth.https://www.mdpi.com/2227-9059/10/9/2149colorectal carcinomaserosal invasionT stageepithelial-mesenchymal transitionmiR-200 familytarget genes |
| spellingShingle | Branislava Ranković Emanuela Boštjančič Nina Zidar Margareta Žlajpah Jera Jeruc <i>miR-200b</i>, <i>ZEB2</i> and <i>PTPN13</i> Are Downregulated in Colorectal Carcinoma with Serosal Invasion Biomedicines colorectal carcinoma serosal invasion T stage epithelial-mesenchymal transition miR-200 family target genes |
| title | <i>miR-200b</i>, <i>ZEB2</i> and <i>PTPN13</i> Are Downregulated in Colorectal Carcinoma with Serosal Invasion |
| title_full | <i>miR-200b</i>, <i>ZEB2</i> and <i>PTPN13</i> Are Downregulated in Colorectal Carcinoma with Serosal Invasion |
| title_fullStr | <i>miR-200b</i>, <i>ZEB2</i> and <i>PTPN13</i> Are Downregulated in Colorectal Carcinoma with Serosal Invasion |
| title_full_unstemmed | <i>miR-200b</i>, <i>ZEB2</i> and <i>PTPN13</i> Are Downregulated in Colorectal Carcinoma with Serosal Invasion |
| title_short | <i>miR-200b</i>, <i>ZEB2</i> and <i>PTPN13</i> Are Downregulated in Colorectal Carcinoma with Serosal Invasion |
| title_sort | i mir 200b i i zeb2 i and i ptpn13 i are downregulated in colorectal carcinoma with serosal invasion |
| topic | colorectal carcinoma serosal invasion T stage epithelial-mesenchymal transition miR-200 family target genes |
| url | https://www.mdpi.com/2227-9059/10/9/2149 |
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