Effects of Lactobacillus Enhanced Fermentation on the Antioxidant Characteristics and Flavor of Tartary Buckwheat Jiaosu

In order to improve the taste and antioxidant capacity of Tartary buckwheat. Using reducing power and SOD activity as main indexes, four strains of Lactobacillus plantarum (LBS8, MRS5), Lactobacillus paracareflora (MC6) and Lactobacillus paracei (SL1) were investigated to enhance the fermentation of...

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Main Authors: Minghong BIAN, Tianmeng YUAN, Nan YANG, Baolin HAN, Dandan HUANG, Qiang XU
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2024-01-01
Series:Shipin gongye ke-ji
Subjects:
Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023020286
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author Minghong BIAN
Tianmeng YUAN
Nan YANG
Baolin HAN
Dandan HUANG
Qiang XU
author_facet Minghong BIAN
Tianmeng YUAN
Nan YANG
Baolin HAN
Dandan HUANG
Qiang XU
author_sort Minghong BIAN
collection DOAJ
description In order to improve the taste and antioxidant capacity of Tartary buckwheat. Using reducing power and SOD activity as main indexes, four strains of Lactobacillus plantarum (LBS8, MRS5), Lactobacillus paracareflora (MC6) and Lactobacillus paracei (SL1) were investigated to enhance the fermentation of Tartary buckwheat Jiaosu. An excellent strain was screened out and the effects of inoculation amount, initial sugar degree, fermentation temperature and solid-liquid ratio on the fermentation process of Tartary buckwheat were studied. The fermentation process of Tartary buckwheat was optimized by completely randomalized design and orthogonal experimental design. The results showed that LBS8 fermentation Tartary buckwheat had strong reducing ability and SOD activity. Inoculation amount of 6%, initial sugar degree of 16°Brix, fermentation temperature of 32 ℃, solid-liquid ratio of 1:7 were the optimum conditions. Under this condition, the reducing power of the obtained Jiaosu was 0.753 mg/mL, the SOD activity was 272.12 U/mL, the total content of flavone was 0.521 mg/mL, the total content of phenol was 2.75 mg/mL, and the content of glutathione was 0.273 mg/mL. Compared with the control group, there were significant improvements. Through GC-MS analysis, tartary buckwheat Jiaosu contained characteristic aroma substances such as ethyl acetate, ethyl linoleate, ethyl undecanoate, and butanedione, giving tartary buckwheat Jiaosu a unique flavor. Compared with commercially available starter cultures, the total flavonoids, polyphenols, glutathione, reducing power and antioxidant capacity of Lactobacillus plantarum enhanced fermentation increased by 247%, 2%, 36.5%, 30.73% and 30.09%, respectively. The flavor and taste of Lactobacillus plantarum enhanced fermentation was richer than that of commercially available starter cultures. The results indicated that inoculation with Lactobacillus plantarum fermentation was beneficial to improve the quality of Tartary buckwheat fermentation.
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spelling doaj.art-3a88e8ac15f9422dae28c9d02a1803862024-01-11T01:27:35ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062024-01-0145211812510.13386/j.issn1002-0306.20230202862023020286-2Effects of Lactobacillus Enhanced Fermentation on the Antioxidant Characteristics and Flavor of Tartary Buckwheat JiaosuMinghong BIAN0Tianmeng YUAN1Nan YANG2Baolin HAN3Dandan HUANG4Qiang XU5College of Bioengineering, Sichuan University of Science and Engineering, Yibin 644000, ChinaCollege of Bioengineering, Sichuan University of Science and Engineering, Yibin 644000, ChinaCollege of Bioengineering, Sichuan University of Science and Engineering, Yibin 644000, ChinaCollege of Bioengineering, Sichuan University of Science and Engineering, Yibin 644000, ChinaNorth Sichuan Medical University, Nanchong 637000, ChinaCollege of Bioengineering, Sichuan University of Science and Engineering, Yibin 644000, ChinaIn order to improve the taste and antioxidant capacity of Tartary buckwheat. Using reducing power and SOD activity as main indexes, four strains of Lactobacillus plantarum (LBS8, MRS5), Lactobacillus paracareflora (MC6) and Lactobacillus paracei (SL1) were investigated to enhance the fermentation of Tartary buckwheat Jiaosu. An excellent strain was screened out and the effects of inoculation amount, initial sugar degree, fermentation temperature and solid-liquid ratio on the fermentation process of Tartary buckwheat were studied. The fermentation process of Tartary buckwheat was optimized by completely randomalized design and orthogonal experimental design. The results showed that LBS8 fermentation Tartary buckwheat had strong reducing ability and SOD activity. Inoculation amount of 6%, initial sugar degree of 16°Brix, fermentation temperature of 32 ℃, solid-liquid ratio of 1:7 were the optimum conditions. Under this condition, the reducing power of the obtained Jiaosu was 0.753 mg/mL, the SOD activity was 272.12 U/mL, the total content of flavone was 0.521 mg/mL, the total content of phenol was 2.75 mg/mL, and the content of glutathione was 0.273 mg/mL. Compared with the control group, there were significant improvements. Through GC-MS analysis, tartary buckwheat Jiaosu contained characteristic aroma substances such as ethyl acetate, ethyl linoleate, ethyl undecanoate, and butanedione, giving tartary buckwheat Jiaosu a unique flavor. Compared with commercially available starter cultures, the total flavonoids, polyphenols, glutathione, reducing power and antioxidant capacity of Lactobacillus plantarum enhanced fermentation increased by 247%, 2%, 36.5%, 30.73% and 30.09%, respectively. The flavor and taste of Lactobacillus plantarum enhanced fermentation was richer than that of commercially available starter cultures. The results indicated that inoculation with Lactobacillus plantarum fermentation was beneficial to improve the quality of Tartary buckwheat fermentation.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023020286lactobacillustartary buckwheatjiaosufermentation processflavor
spellingShingle Minghong BIAN
Tianmeng YUAN
Nan YANG
Baolin HAN
Dandan HUANG
Qiang XU
Effects of Lactobacillus Enhanced Fermentation on the Antioxidant Characteristics and Flavor of Tartary Buckwheat Jiaosu
Shipin gongye ke-ji
lactobacillus
tartary buckwheat
jiaosu
fermentation process
flavor
title Effects of Lactobacillus Enhanced Fermentation on the Antioxidant Characteristics and Flavor of Tartary Buckwheat Jiaosu
title_full Effects of Lactobacillus Enhanced Fermentation on the Antioxidant Characteristics and Flavor of Tartary Buckwheat Jiaosu
title_fullStr Effects of Lactobacillus Enhanced Fermentation on the Antioxidant Characteristics and Flavor of Tartary Buckwheat Jiaosu
title_full_unstemmed Effects of Lactobacillus Enhanced Fermentation on the Antioxidant Characteristics and Flavor of Tartary Buckwheat Jiaosu
title_short Effects of Lactobacillus Enhanced Fermentation on the Antioxidant Characteristics and Flavor of Tartary Buckwheat Jiaosu
title_sort effects of lactobacillus enhanced fermentation on the antioxidant characteristics and flavor of tartary buckwheat jiaosu
topic lactobacillus
tartary buckwheat
jiaosu
fermentation process
flavor
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023020286
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