PDMS Organ-On-Chip Design and Fabrication: Strategies for Improving Fluidic Integration and Chip Robustness of Rapidly Prototyped Microfluidic In Vitro Models
The PDMS-based microfluidic organ-on-chip platform represents an exciting paradigm that has enjoyed a rapid rise in popularity and adoption. A particularly promising element of this platform is its amenability to rapid manufacturing strategies, which can enable quick adaptations through iterative pr...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/13/10/1573 |
| _version_ | 1797471296079003648 |
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| author | Tiffany C. Cameron Avineet Randhawa Samantha M. Grist Tanya Bennet Jessica Hua Luis G. Alde Tara M. Caffrey Cheryl L. Wellington Karen C. Cheung |
| author_facet | Tiffany C. Cameron Avineet Randhawa Samantha M. Grist Tanya Bennet Jessica Hua Luis G. Alde Tara M. Caffrey Cheryl L. Wellington Karen C. Cheung |
| author_sort | Tiffany C. Cameron |
| collection | DOAJ |
| description | The PDMS-based microfluidic organ-on-chip platform represents an exciting paradigm that has enjoyed a rapid rise in popularity and adoption. A particularly promising element of this platform is its amenability to rapid manufacturing strategies, which can enable quick adaptations through iterative prototyping. These strategies, however, come with challenges; fluid flow, for example, a core principle of organs-on-chip and the physiology they aim to model, necessitates robust, leak-free channels for potentially long (multi-week) culture durations. In this report, we describe microfluidic chip fabrication methods and strategies that are aimed at overcoming these difficulties; we employ a subset of these strategies to a blood–brain-barrier-on-chip, with others applied to a small-airway-on-chip. Design approaches are detailed with considerations presented for readers. Results pertaining to fabrication parameters we aimed to improve (e.g., the thickness uniformity of molded PDMS), as well as illustrative results pertaining to the establishment of cell cultures using these methods will also be presented. |
| first_indexed | 2024-03-09T19:47:07Z |
| format | Article |
| id | doaj.art-3a890dbf09b44c7bbd5956a26ea1a9f1 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T19:47:07Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-3a890dbf09b44c7bbd5956a26ea1a9f12023-11-24T01:20:50ZengMDPI AGMicromachines2072-666X2022-09-011310157310.3390/mi13101573PDMS Organ-On-Chip Design and Fabrication: Strategies for Improving Fluidic Integration and Chip Robustness of Rapidly Prototyped Microfluidic In Vitro ModelsTiffany C. Cameron0Avineet Randhawa1Samantha M. Grist2Tanya Bennet3Jessica Hua4Luis G. Alde5Tara M. Caffrey6Cheryl L. Wellington7Karen C. Cheung8School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, CanadaSchool of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, CanadaSchool of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, CanadaSchool of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, CanadaSchool of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, CanadaCentre for Blood Research, University of British Columbia, Vancouver, BC V6T 1Z3, CanadaSchool of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, CanadaDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC V6T 2B5, CanadaSchool of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, CanadaThe PDMS-based microfluidic organ-on-chip platform represents an exciting paradigm that has enjoyed a rapid rise in popularity and adoption. A particularly promising element of this platform is its amenability to rapid manufacturing strategies, which can enable quick adaptations through iterative prototyping. These strategies, however, come with challenges; fluid flow, for example, a core principle of organs-on-chip and the physiology they aim to model, necessitates robust, leak-free channels for potentially long (multi-week) culture durations. In this report, we describe microfluidic chip fabrication methods and strategies that are aimed at overcoming these difficulties; we employ a subset of these strategies to a blood–brain-barrier-on-chip, with others applied to a small-airway-on-chip. Design approaches are detailed with considerations presented for readers. Results pertaining to fabrication parameters we aimed to improve (e.g., the thickness uniformity of molded PDMS), as well as illustrative results pertaining to the establishment of cell cultures using these methods will also be presented.https://www.mdpi.com/2072-666X/13/10/1573microfluidicorgan-on-chiprapid prototypingcell culture |
| spellingShingle | Tiffany C. Cameron Avineet Randhawa Samantha M. Grist Tanya Bennet Jessica Hua Luis G. Alde Tara M. Caffrey Cheryl L. Wellington Karen C. Cheung PDMS Organ-On-Chip Design and Fabrication: Strategies for Improving Fluidic Integration and Chip Robustness of Rapidly Prototyped Microfluidic In Vitro Models Micromachines microfluidic organ-on-chip rapid prototyping cell culture |
| title | PDMS Organ-On-Chip Design and Fabrication: Strategies for Improving Fluidic Integration and Chip Robustness of Rapidly Prototyped Microfluidic In Vitro Models |
| title_full | PDMS Organ-On-Chip Design and Fabrication: Strategies for Improving Fluidic Integration and Chip Robustness of Rapidly Prototyped Microfluidic In Vitro Models |
| title_fullStr | PDMS Organ-On-Chip Design and Fabrication: Strategies for Improving Fluidic Integration and Chip Robustness of Rapidly Prototyped Microfluidic In Vitro Models |
| title_full_unstemmed | PDMS Organ-On-Chip Design and Fabrication: Strategies for Improving Fluidic Integration and Chip Robustness of Rapidly Prototyped Microfluidic In Vitro Models |
| title_short | PDMS Organ-On-Chip Design and Fabrication: Strategies for Improving Fluidic Integration and Chip Robustness of Rapidly Prototyped Microfluidic In Vitro Models |
| title_sort | pdms organ on chip design and fabrication strategies for improving fluidic integration and chip robustness of rapidly prototyped microfluidic in vitro models |
| topic | microfluidic organ-on-chip rapid prototyping cell culture |
| url | https://www.mdpi.com/2072-666X/13/10/1573 |
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