Recombinant Mosquito Densovirus with <i>Bti</i> Toxins Significantly Improves Pathogenicity against <i>Aedes albopictus</i>
Mosquito densoviruses (MDVs) are mosquito-specific viruses that are recommended as mosquito bio-control agents. The MDV <i>Aedes aegypti</i> densovirus (AeDNV) is a good candidate for controlling mosquitoes. However, the slow activity restricts their widespread use for vector control. In...
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MDPI AG
2022-02-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/2/147 |
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| author | Khadija Batool Intikhab Alam Peiwen Liu Zeng Shu Siyu Zhao Wenqiang Yang Xiao Jie Jinbao Gu Xiao-Guang Chen |
| author_facet | Khadija Batool Intikhab Alam Peiwen Liu Zeng Shu Siyu Zhao Wenqiang Yang Xiao Jie Jinbao Gu Xiao-Guang Chen |
| author_sort | Khadija Batool |
| collection | DOAJ |
| description | Mosquito densoviruses (MDVs) are mosquito-specific viruses that are recommended as mosquito bio-control agents. The MDV <i>Aedes aegypti</i> densovirus (AeDNV) is a good candidate for controlling mosquitoes. However, the slow activity restricts their widespread use for vector control. In this study, we introduced the <i>Bacillus thuringiensis</i> (<i>Bti</i>) toxin Cry11Aa domain II loop α8 and Cyt1Aa loop β6-αE peptides into the AeDNV genome to improve its mosquitocidal efficiency; protein expression was confirmed using nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS). Recombinant plasmids were transfected into mosquito C6/36 cell lines, and the expression of specific peptides was detected through RT-PCR. A toxicity bioassay against the first instar <i>Aedes albopictus</i> larvae revealed that the pathogenic activity of recombinant AeDNV was significantly higher and faster than the wild-type (<i>wt</i>) viruses, and mortality increased in a dose-dependent manner. The recombinant viruses were genetically stable and displayed growth phenotype and virus proliferation ability, similar to wild-type AeDNV. Our novel results offer further insights by combining two mosquitocidal pathogens to improve viral toxicity for mosquito control. |
| first_indexed | 2024-03-09T20:55:17Z |
| format | Article |
| id | doaj.art-3a8948f4f7b94bfa9ce00d8a54ad6d3a |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T20:55:17Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-3a8948f4f7b94bfa9ce00d8a54ad6d3a2023-11-23T22:22:09ZengMDPI AGToxins2072-66512022-02-0114214710.3390/toxins14020147Recombinant Mosquito Densovirus with <i>Bti</i> Toxins Significantly Improves Pathogenicity against <i>Aedes albopictus</i>Khadija Batool0Intikhab Alam1Peiwen Liu2Zeng Shu3Siyu Zhao4Wenqiang Yang5Xiao Jie6Jinbao Gu7Xiao-Guang Chen8Department of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, ChinaCollege of Life Sciences, South China Agricultural University, Guangzhou 510515, ChinaDepartment of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, ChinaDepartment of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, ChinaDepartment of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, ChinaDepartment of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, ChinaDepartment of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, ChinaDepartment of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, ChinaDepartment of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, ChinaMosquito densoviruses (MDVs) are mosquito-specific viruses that are recommended as mosquito bio-control agents. The MDV <i>Aedes aegypti</i> densovirus (AeDNV) is a good candidate for controlling mosquitoes. However, the slow activity restricts their widespread use for vector control. In this study, we introduced the <i>Bacillus thuringiensis</i> (<i>Bti</i>) toxin Cry11Aa domain II loop α8 and Cyt1Aa loop β6-αE peptides into the AeDNV genome to improve its mosquitocidal efficiency; protein expression was confirmed using nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS). Recombinant plasmids were transfected into mosquito C6/36 cell lines, and the expression of specific peptides was detected through RT-PCR. A toxicity bioassay against the first instar <i>Aedes albopictus</i> larvae revealed that the pathogenic activity of recombinant AeDNV was significantly higher and faster than the wild-type (<i>wt</i>) viruses, and mortality increased in a dose-dependent manner. The recombinant viruses were genetically stable and displayed growth phenotype and virus proliferation ability, similar to wild-type AeDNV. Our novel results offer further insights by combining two mosquitocidal pathogens to improve viral toxicity for mosquito control.https://www.mdpi.com/2072-6651/14/2/147<i>Aedes aegypti</i> densovirus<i>Bacillus thuringiensis</i>toxicity<i>Ae. albopictus</i> |
| spellingShingle | Khadija Batool Intikhab Alam Peiwen Liu Zeng Shu Siyu Zhao Wenqiang Yang Xiao Jie Jinbao Gu Xiao-Guang Chen Recombinant Mosquito Densovirus with <i>Bti</i> Toxins Significantly Improves Pathogenicity against <i>Aedes albopictus</i> Toxins <i>Aedes aegypti</i> densovirus <i>Bacillus thuringiensis</i> toxicity <i>Ae. albopictus</i> |
| title | Recombinant Mosquito Densovirus with <i>Bti</i> Toxins Significantly Improves Pathogenicity against <i>Aedes albopictus</i> |
| title_full | Recombinant Mosquito Densovirus with <i>Bti</i> Toxins Significantly Improves Pathogenicity against <i>Aedes albopictus</i> |
| title_fullStr | Recombinant Mosquito Densovirus with <i>Bti</i> Toxins Significantly Improves Pathogenicity against <i>Aedes albopictus</i> |
| title_full_unstemmed | Recombinant Mosquito Densovirus with <i>Bti</i> Toxins Significantly Improves Pathogenicity against <i>Aedes albopictus</i> |
| title_short | Recombinant Mosquito Densovirus with <i>Bti</i> Toxins Significantly Improves Pathogenicity against <i>Aedes albopictus</i> |
| title_sort | recombinant mosquito densovirus with i bti i toxins significantly improves pathogenicity against i aedes albopictus i |
| topic | <i>Aedes aegypti</i> densovirus <i>Bacillus thuringiensis</i> toxicity <i>Ae. albopictus</i> |
| url | https://www.mdpi.com/2072-6651/14/2/147 |
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