EBV and MSI Status in Gastric Cancer: Does It Matter?
We investigated the impactof microsatellite instability (MSI) and Epstein–Barr virus (EBV) status in gastric cancer (GC), regarding response to perioperative chemotherapy (POPChT), overall survival (OS), and progression-free survival (PFS). We included 137 cases of operated GC, 51 of which were subm...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/15/1/74 |
| _version_ | 1797626170004471808 |
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| author | Catarina Neto do Nascimento Luís Mascarenhas-Lemos João Ricardo Silva Diogo Sousa Marques Catarina Ferreira Gouveia Ana Faria Sónia Velho Rita Garrido Rui Maio Andreia Costa Patrícia Pontes Xiaogang Wen Irene Gullo Marília Cravo Fátima Carneiro |
| author_facet | Catarina Neto do Nascimento Luís Mascarenhas-Lemos João Ricardo Silva Diogo Sousa Marques Catarina Ferreira Gouveia Ana Faria Sónia Velho Rita Garrido Rui Maio Andreia Costa Patrícia Pontes Xiaogang Wen Irene Gullo Marília Cravo Fátima Carneiro |
| author_sort | Catarina Neto do Nascimento |
| collection | DOAJ |
| description | We investigated the impactof microsatellite instability (MSI) and Epstein–Barr virus (EBV) status in gastric cancer (GC), regarding response to perioperative chemotherapy (POPChT), overall survival (OS), and progression-free survival (PFS). We included 137 cases of operated GC, 51 of which were submitted to POPChT. MSI status was determined by multiplex PCR and EBV status by EBV-encoded RNA in situ hybridization. Thirty-seven (27%) cases presented as MSI-high, and seven (5.1%) were EBV+. Concerning tumor regression after POPChT, no differences were observed between the molecular subtypes, but females were more likely to respond (<i>p</i> = 0.062). No significant differences were found in OS or PFS between different subtypes. In multivariate analysis, age (HR 1.02, IC 95% 1.002–1.056, <i>p</i> = 0.033) and positive lymph nodes (HR 1.82, IC 95% 1.034–3.211, <i>p</i> = 0.038) were the only prognostic factors for OS. However, females with MSI-high tumors treated with POPChT demonstrated a significantly increased OS compared to females with MSS tumors (<i>p</i> = 0.031). In conclusion, we found a high proportion of MSI-high cases. MSI and EBV status did not influence OS or PFS either in patients submitted to POPChT or surgery alone. However, superior survival of females with MSI-high tumors suggests that sex disparities and molecular classification may influence treatment options in GC. |
| first_indexed | 2024-03-11T10:06:41Z |
| format | Article |
| id | doaj.art-3a8963b165984f178f616efe0a1e42cb |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T10:06:41Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-3a8963b165984f178f616efe0a1e42cb2023-11-16T15:01:16ZengMDPI AGCancers2072-66942022-12-011517410.3390/cancers15010074EBV and MSI Status in Gastric Cancer: Does It Matter?Catarina Neto do Nascimento0Luís Mascarenhas-Lemos1João Ricardo Silva2Diogo Sousa Marques3Catarina Ferreira Gouveia4Ana Faria5Sónia Velho6Rita Garrido7Rui Maio8Andreia Costa9Patrícia Pontes10Xiaogang Wen11Irene Gullo12Marília Cravo13Fátima Carneiro14Department of Gastroenterology, Hospital Beatriz Ângelo, 2674-514 Loures, PortugalFaculty of Medicine, Catholic University of Portugal, 2635-631 Rio de Mouro, PortugalFaculty of Medicine, University of Porto (FMUP), 4200-319 Porto, PortugalFaculty of Medicine, University of Porto (FMUP), 4200-319 Porto, PortugalDepartment of Gastroenterology, Hospital Beatriz Ângelo, 2674-514 Loures, PortugalDepartment of Oncology, Hospital Beatriz Ângelo, 2674-514 Loures, PortugalDepartment of Dietetics and Nutrition, Hospital Beatriz Ângelo, 2674-514 Loures, PortugalDepartment of General Surgery, Hospital Beatriz Ângelo, 2674-514 Loures, PortugalDepartment of General Surgery, Hospital Beatriz Ângelo, 2674-514 Loures, PortugalDepartment of Oncology, Centro Hospitalar Universitário de São João (CHUSJ), 4200-319 Porto, PortugalDepartment of Pathology, Centro Hospitalar Universitário de São João (CHUSJ), 4200-319 Porto, Portugali3S—Instituto de Investigação e Inovação em Saúde and Institute of Molecular Pathology and Immunology, University of Porto (Ipatimup), 4200-135 Porto, PortugalFaculty of Medicine, University of Porto (FMUP), 4200-319 Porto, PortugalDepartment of Gastroenterology, Hospital da Luz de Lisboa, 1500-650 Lisboa, PortugalFaculty of Medicine, University of Porto (FMUP), 4200-319 Porto, PortugalWe investigated the impactof microsatellite instability (MSI) and Epstein–Barr virus (EBV) status in gastric cancer (GC), regarding response to perioperative chemotherapy (POPChT), overall survival (OS), and progression-free survival (PFS). We included 137 cases of operated GC, 51 of which were submitted to POPChT. MSI status was determined by multiplex PCR and EBV status by EBV-encoded RNA in situ hybridization. Thirty-seven (27%) cases presented as MSI-high, and seven (5.1%) were EBV+. Concerning tumor regression after POPChT, no differences were observed between the molecular subtypes, but females were more likely to respond (<i>p</i> = 0.062). No significant differences were found in OS or PFS between different subtypes. In multivariate analysis, age (HR 1.02, IC 95% 1.002–1.056, <i>p</i> = 0.033) and positive lymph nodes (HR 1.82, IC 95% 1.034–3.211, <i>p</i> = 0.038) were the only prognostic factors for OS. However, females with MSI-high tumors treated with POPChT demonstrated a significantly increased OS compared to females with MSS tumors (<i>p</i> = 0.031). In conclusion, we found a high proportion of MSI-high cases. MSI and EBV status did not influence OS or PFS either in patients submitted to POPChT or surgery alone. However, superior survival of females with MSI-high tumors suggests that sex disparities and molecular classification may influence treatment options in GC.https://www.mdpi.com/2072-6694/15/1/74gastric cancerEpstein–Barr virusmicrosatellite instabilityneoadjuvant chemotherapyperioperative chemotherapy predictorprognosis |
| spellingShingle | Catarina Neto do Nascimento Luís Mascarenhas-Lemos João Ricardo Silva Diogo Sousa Marques Catarina Ferreira Gouveia Ana Faria Sónia Velho Rita Garrido Rui Maio Andreia Costa Patrícia Pontes Xiaogang Wen Irene Gullo Marília Cravo Fátima Carneiro EBV and MSI Status in Gastric Cancer: Does It Matter? Cancers gastric cancer Epstein–Barr virus microsatellite instability neoadjuvant chemotherapy perioperative chemotherapy predictor prognosis |
| title | EBV and MSI Status in Gastric Cancer: Does It Matter? |
| title_full | EBV and MSI Status in Gastric Cancer: Does It Matter? |
| title_fullStr | EBV and MSI Status in Gastric Cancer: Does It Matter? |
| title_full_unstemmed | EBV and MSI Status in Gastric Cancer: Does It Matter? |
| title_short | EBV and MSI Status in Gastric Cancer: Does It Matter? |
| title_sort | ebv and msi status in gastric cancer does it matter |
| topic | gastric cancer Epstein–Barr virus microsatellite instability neoadjuvant chemotherapy perioperative chemotherapy predictor prognosis |
| url | https://www.mdpi.com/2072-6694/15/1/74 |
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