Molecular isolation and identification of Mycobacterium avium subsp. hominissuis in Didelphis virginiana from Hidalgo, Mexico
Objective: To isolate and identify the exact species of the genus Mycobacterium from Didelphis (D.) virginiana, and the direct implications of this bacterium to public health and veterinary medicine. Methods: Thirty-one D. virginiana were captured and necropsied in Hidalgo, Mexico. Tissue samples we...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | Asian Pacific Journal of Tropical Medicine |
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| Online Access: | http://www.apjtm.org/article.asp?issn=1995-7645;year=2020;volume=13;issue=3;spage=131;epage=136;aulast=Rivero-Perez |
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| author | Nallely Rivero-Perez Juan Ocampo-López Benjamín Valladares-Carranza Fabián R Gómez de Anda Francisco J Peña Jiménez Victor M Martínez Juárez Armando Peláez Acero José I Olave Leyva Deyanira Ojeda-Ramírez Adrian Zaragoza-Bastida |
| author_facet | Nallely Rivero-Perez Juan Ocampo-López Benjamín Valladares-Carranza Fabián R Gómez de Anda Francisco J Peña Jiménez Victor M Martínez Juárez Armando Peláez Acero José I Olave Leyva Deyanira Ojeda-Ramírez Adrian Zaragoza-Bastida |
| author_sort | Nallely Rivero-Perez |
| collection | DOAJ |
| description | Objective: To isolate and identify the exact species of the genus Mycobacterium from Didelphis (D.) virginiana, and the direct implications of this bacterium to public health and veterinary medicine.
Methods: Thirty-one D. virginiana were captured and necropsied in Hidalgo, Mexico. Tissue samples were collected to culture mycobacteria present and examine individual specimens’ histopathology. Mycobacterium identification was obtained through the application of amplification and sequencing of 16S rDNA techniques.
Results: Three strains were isolated and identified as Mycobacterium (M.) avium subsp. hominissuis by utilizing M. avium complex- specific primers. Granulomatous lesions were observed in the subpleural zone (granuloma grade Π ) and bronchial (granuloma grade I ) of the lungs of D. virginiana with positive isolation.
Conclusions: Three strains of M. avium subsp. hominissuis, from lung tissue samples of D. virginiana were identified. This subspecies of M. avium has important implications in public health and veterinary medicine. |
| first_indexed | 2024-12-23T21:30:30Z |
| format | Article |
| id | doaj.art-3a89974112b842f38f5e2cb5c37ceae3 |
| institution | Directory Open Access Journal |
| issn | 2352-4146 |
| language | English |
| last_indexed | 2024-12-23T21:30:30Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Medicine |
| spelling | doaj.art-3a89974112b842f38f5e2cb5c37ceae32022-12-21T17:30:29ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Medicine2352-41462020-01-0113313113610.4103/1995-7645.278098Molecular isolation and identification of Mycobacterium avium subsp. hominissuis in Didelphis virginiana from Hidalgo, MexicoNallely Rivero-PerezJuan Ocampo-LópezBenjamín Valladares-CarranzaFabián R Gómez de AndaFrancisco J Peña JiménezVictor M Martínez JuárezArmando Peláez AceroJosé I Olave LeyvaDeyanira Ojeda-RamírezAdrian Zaragoza-BastidaObjective: To isolate and identify the exact species of the genus Mycobacterium from Didelphis (D.) virginiana, and the direct implications of this bacterium to public health and veterinary medicine. Methods: Thirty-one D. virginiana were captured and necropsied in Hidalgo, Mexico. Tissue samples were collected to culture mycobacteria present and examine individual specimens’ histopathology. Mycobacterium identification was obtained through the application of amplification and sequencing of 16S rDNA techniques. Results: Three strains were isolated and identified as Mycobacterium (M.) avium subsp. hominissuis by utilizing M. avium complex- specific primers. Granulomatous lesions were observed in the subpleural zone (granuloma grade Π ) and bronchial (granuloma grade I ) of the lungs of D. virginiana with positive isolation. Conclusions: Three strains of M. avium subsp. hominissuis, from lung tissue samples of D. virginiana were identified. This subspecies of M. avium has important implications in public health and veterinary medicine.http://www.apjtm.org/article.asp?issn=1995-7645;year=2020;volume=13;issue=3;spage=131;epage=136;aulast=Rivero-Perezdidelphis virginiana; mycobacterium avium complex; mycobacterium avium subsp. hominissuis; public health |
| spellingShingle | Nallely Rivero-Perez Juan Ocampo-López Benjamín Valladares-Carranza Fabián R Gómez de Anda Francisco J Peña Jiménez Victor M Martínez Juárez Armando Peláez Acero José I Olave Leyva Deyanira Ojeda-Ramírez Adrian Zaragoza-Bastida Molecular isolation and identification of Mycobacterium avium subsp. hominissuis in Didelphis virginiana from Hidalgo, Mexico Asian Pacific Journal of Tropical Medicine didelphis virginiana; mycobacterium avium complex; mycobacterium avium subsp. hominissuis; public health |
| title | Molecular isolation and identification of Mycobacterium avium subsp. hominissuis in Didelphis virginiana from Hidalgo, Mexico |
| title_full | Molecular isolation and identification of Mycobacterium avium subsp. hominissuis in Didelphis virginiana from Hidalgo, Mexico |
| title_fullStr | Molecular isolation and identification of Mycobacterium avium subsp. hominissuis in Didelphis virginiana from Hidalgo, Mexico |
| title_full_unstemmed | Molecular isolation and identification of Mycobacterium avium subsp. hominissuis in Didelphis virginiana from Hidalgo, Mexico |
| title_short | Molecular isolation and identification of Mycobacterium avium subsp. hominissuis in Didelphis virginiana from Hidalgo, Mexico |
| title_sort | molecular isolation and identification of mycobacterium avium subsp hominissuis in didelphis virginiana from hidalgo mexico |
| topic | didelphis virginiana; mycobacterium avium complex; mycobacterium avium subsp. hominissuis; public health |
| url | http://www.apjtm.org/article.asp?issn=1995-7645;year=2020;volume=13;issue=3;spage=131;epage=136;aulast=Rivero-Perez |
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