Chemical composition of pumpkin (Cucurbita maxima) seeds and its supplemental effect on Indian women with metabolic syndrome
This study aimed to investigate the effect of pumpkin (Cucurbita maxima) seed supplementation on the anthropometric measurements, biochemical parameters, and blood pressure (BP) of Indian women with metabolic syndrome (MetS). Initially, in vitro antioxidant activities of pumpkin seeds extract were a...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Arabian Journal of Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S187853522200301X |
| _version_ | 1818469017216614400 |
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| author | Sarah Jane Monica Sheila John R. Madhanagopal C. Sivaraj Ameer Khusro P. Arumugam Márió Gajdács D. Esther Lydia Muhammad Umar Khayam Sahibzada Saad Alghamdi Mazen Almehmadi Talha Bin Emran |
| author_facet | Sarah Jane Monica Sheila John R. Madhanagopal C. Sivaraj Ameer Khusro P. Arumugam Márió Gajdács D. Esther Lydia Muhammad Umar Khayam Sahibzada Saad Alghamdi Mazen Almehmadi Talha Bin Emran |
| author_sort | Sarah Jane Monica |
| collection | DOAJ |
| description | This study aimed to investigate the effect of pumpkin (Cucurbita maxima) seed supplementation on the anthropometric measurements, biochemical parameters, and blood pressure (BP) of Indian women with metabolic syndrome (MetS). Initially, in vitro antioxidant activities of pumpkin seeds extract were assessed using standard methods. In vitro alpha-amylase, alpha-glucosidase, and dipeptidyl peptidase IV (DPP-IV) inhibition effects, along with glucose uptake assay using 3T3-L1 cell lines were performed to determine the antidiabetic effects of the seeds extract. Fatty acids and phytoconstituents were identified using gas chromatography-mass spectrometry (GC–MS). Indian women aged 30–50 years, having MetS were assigned either to intervention (n = 21) or control (n = 21) group on a random basis. Participants in the intervention group received 5 g of pumpkin seeds for 60 days. Participants in both intervention and control were advised to follow certain dietary guidelines throughout the study. Pumpkin seeds extract exhibited not only strong reducing power but also scavenged DPPH and ABTS●+ free radicals with low IC50 values. Pumpkin seeds inhibited alpha-amylase, alpha-glucosidase, and DPP-IV enzymes at varying concentrations with IC50 values of 138, 22, and 246 µg/mL, respectively. Furthermore, glucose uptake was enhanced by 213% at 300 ng/mL on the 3T3-L1 cell line. GC–MS analysis showed the presence of propyl piperidine, flavone, oleic acid, and methyl esters of fatty acids in the seed extract. On comparing the changes in mean reduction/ increment in the anthropometric measurements as well as biochemical parameters and BP between the groups, significant difference (P = 0.012) was observed only for fasting plasma glucose. Findings of the present study highlight the role of pumpkin seeds as a cost-effective adjunct in treating MetS. |
| first_indexed | 2024-04-13T21:19:20Z |
| format | Article |
| id | doaj.art-3a8a86ff5ee940ed8da798f10a8e16eb |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-13T21:19:20Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-3a8a86ff5ee940ed8da798f10a8e16eb2022-12-22T02:29:34ZengElsevierArabian Journal of Chemistry1878-53522022-08-01158103985Chemical composition of pumpkin (Cucurbita maxima) seeds and its supplemental effect on Indian women with metabolic syndromeSarah Jane Monica0Sheila John1R. Madhanagopal2C. Sivaraj3Ameer Khusro4P. Arumugam5Márió Gajdács6D. Esther Lydia7Muhammad Umar Khayam Sahibzada8Saad Alghamdi9Mazen Almehmadi10Talha Bin Emran11Department of Home Science, Women’s Christian College (Autonomous), Chennai, Tamil Nadu, India; Corresponding authors.Department of Home Science, Women’s Christian College (Autonomous), Chennai, Tamil Nadu, IndiaDepartment of Statistics, The Madura College (Autonomous), Madurai, Tamil Nadu, IndiaArmats Biotek Training and Research Institute, Chennai, Tamil Nadu, IndiaResearch Department of Plant Biology and Plant Biotechnology, Loyola College (Autonomous) Chennai, Tamil Nadu, India; Corresponding authors.Armats Biotek Training and Research Institute, Chennai, Tamil Nadu, IndiaDepartment of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Tisza Lajos krt. 63., 6720 Szeged, Hungary; Corresponding authors.PG Food Chemistry and Food Processing, Loyola College (Autonomous) Chennai, Tamil Nadu, IndiaDepartment of Pharmacy, The Sahara College Narowal, Narowal, Punjab, PakistanLaboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Pharmacy, BGC Trust University Bangladesh, Chittagong 4381, Bangladesh; Department of Pharmacy, Faculty of Allied Health Sciences, Daffodil International University, Dhaka 1207, BangladeshThis study aimed to investigate the effect of pumpkin (Cucurbita maxima) seed supplementation on the anthropometric measurements, biochemical parameters, and blood pressure (BP) of Indian women with metabolic syndrome (MetS). Initially, in vitro antioxidant activities of pumpkin seeds extract were assessed using standard methods. In vitro alpha-amylase, alpha-glucosidase, and dipeptidyl peptidase IV (DPP-IV) inhibition effects, along with glucose uptake assay using 3T3-L1 cell lines were performed to determine the antidiabetic effects of the seeds extract. Fatty acids and phytoconstituents were identified using gas chromatography-mass spectrometry (GC–MS). Indian women aged 30–50 years, having MetS were assigned either to intervention (n = 21) or control (n = 21) group on a random basis. Participants in the intervention group received 5 g of pumpkin seeds for 60 days. Participants in both intervention and control were advised to follow certain dietary guidelines throughout the study. Pumpkin seeds extract exhibited not only strong reducing power but also scavenged DPPH and ABTS●+ free radicals with low IC50 values. Pumpkin seeds inhibited alpha-amylase, alpha-glucosidase, and DPP-IV enzymes at varying concentrations with IC50 values of 138, 22, and 246 µg/mL, respectively. Furthermore, glucose uptake was enhanced by 213% at 300 ng/mL on the 3T3-L1 cell line. GC–MS analysis showed the presence of propyl piperidine, flavone, oleic acid, and methyl esters of fatty acids in the seed extract. On comparing the changes in mean reduction/ increment in the anthropometric measurements as well as biochemical parameters and BP between the groups, significant difference (P = 0.012) was observed only for fasting plasma glucose. Findings of the present study highlight the role of pumpkin seeds as a cost-effective adjunct in treating MetS.http://www.sciencedirect.com/science/article/pii/S187853522200301XAntioxidantAntidiabeticIndian womenMetabolic syndromePumpkin seeds |
| spellingShingle | Sarah Jane Monica Sheila John R. Madhanagopal C. Sivaraj Ameer Khusro P. Arumugam Márió Gajdács D. Esther Lydia Muhammad Umar Khayam Sahibzada Saad Alghamdi Mazen Almehmadi Talha Bin Emran Chemical composition of pumpkin (Cucurbita maxima) seeds and its supplemental effect on Indian women with metabolic syndrome Arabian Journal of Chemistry Antioxidant Antidiabetic Indian women Metabolic syndrome Pumpkin seeds |
| title | Chemical composition of pumpkin (Cucurbita maxima) seeds and its supplemental effect on Indian women with metabolic syndrome |
| title_full | Chemical composition of pumpkin (Cucurbita maxima) seeds and its supplemental effect on Indian women with metabolic syndrome |
| title_fullStr | Chemical composition of pumpkin (Cucurbita maxima) seeds and its supplemental effect on Indian women with metabolic syndrome |
| title_full_unstemmed | Chemical composition of pumpkin (Cucurbita maxima) seeds and its supplemental effect on Indian women with metabolic syndrome |
| title_short | Chemical composition of pumpkin (Cucurbita maxima) seeds and its supplemental effect on Indian women with metabolic syndrome |
| title_sort | chemical composition of pumpkin cucurbita maxima seeds and its supplemental effect on indian women with metabolic syndrome |
| topic | Antioxidant Antidiabetic Indian women Metabolic syndrome Pumpkin seeds |
| url | http://www.sciencedirect.com/science/article/pii/S187853522200301X |
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