Amyloid-Beta Peptide Is Toxic to Neurons In Vivo via Indirect Mechanisms
We have studied the neurotoxicity of amyloid-beta (Aβ) after a single unilateral intravitreal injection. Within the retina apoptotic cells were seen throughout the photoreceptor layer and the inner nuclear layer but not in the ganglion cell layer at 48 h after injection of Aβ1-42 compared to vehicle...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2002-06-01
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| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996102904857 |
| _version_ | 1819119490350186496 |
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| author | Desmond T. Walsh Rosa M. Montero Laura G. Bresciani Angela Y.T. Jen Pascale D. Leclercq Debbie Saunders Ahmed N. EL-Amir Lukemann Gbadamoshi Stephen M. Gentleman Ling-Sun Jen |
| author_facet | Desmond T. Walsh Rosa M. Montero Laura G. Bresciani Angela Y.T. Jen Pascale D. Leclercq Debbie Saunders Ahmed N. EL-Amir Lukemann Gbadamoshi Stephen M. Gentleman Ling-Sun Jen |
| author_sort | Desmond T. Walsh |
| collection | DOAJ |
| description | We have studied the neurotoxicity of amyloid-beta (Aβ) after a single unilateral intravitreal injection. Within the retina apoptotic cells were seen throughout the photoreceptor layer and the inner nuclear layer but not in the ganglion cell layer at 48 h after injection of Aβ1-42 compared to vehicle control and control peptide. At 5 months, there was a significant reduction in total cell numbers in the ganglion cell layer in Nissl stained retinas. There was glial cell dysfunction with upregulation of glial fibrillary acidic protein and a reduction in the expression of Müller cell associated proteins in the injected retinas. These results suggest an indirect cytotoxic effect of Aβ on retinal neurons and an important role for dysfunction of Müller glia in mediating Aβ neurotoxicity. |
| first_indexed | 2024-12-22T06:05:36Z |
| format | Article |
| id | doaj.art-3a8d57f3165b469a9d297e22dcd8aff4 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-22T06:05:36Z |
| publishDate | 2002-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-3a8d57f3165b469a9d297e22dcd8aff42022-12-21T18:36:26ZengElsevierNeurobiology of Disease1095-953X2002-06-011012027Amyloid-Beta Peptide Is Toxic to Neurons In Vivo via Indirect MechanismsDesmond T. Walsh0Rosa M. Montero1Laura G. Bresciani2Angela Y.T. Jen3Pascale D. Leclercq4Debbie Saunders5Ahmed N. EL-Amir6Lukemann Gbadamoshi7Stephen M. Gentleman8Ling-Sun Jen9Department of Neuroinflammation, Division of Neuroscience and Psychological Medicine, Imperial College School of Medicine, Charing Cross Hospital, London, W6 8RF, United KingdomDepartment of Neuroinflammation, Division of Neuroscience and Psychological Medicine, Imperial College School of Medicine, Charing Cross Hospital, London, W6 8RF, United KingdomDepartment of Neuroinflammation, Division of Neuroscience and Psychological Medicine, Imperial College School of Medicine, Charing Cross Hospital, London, W6 8RF, United KingdomDepartment of Neuroinflammation, Division of Neuroscience and Psychological Medicine, Imperial College School of Medicine, Charing Cross Hospital, London, W6 8RF, United KingdomDepartment of Neuroinflammation, Division of Neuroscience and Psychological Medicine, Imperial College School of Medicine, Charing Cross Hospital, London, W6 8RF, United KingdomDepartment of Neuroinflammation, Division of Neuroscience and Psychological Medicine, Imperial College School of Medicine, Charing Cross Hospital, London, W6 8RF, United KingdomDepartment of Neuroinflammation, Division of Neuroscience and Psychological Medicine, Imperial College School of Medicine, Charing Cross Hospital, London, W6 8RF, United KingdomDepartment of Neuroinflammation, Division of Neuroscience and Psychological Medicine, Imperial College School of Medicine, Charing Cross Hospital, London, W6 8RF, United KingdomDepartment of Neuroinflammation, Division of Neuroscience and Psychological Medicine, Imperial College School of Medicine, Charing Cross Hospital, London, W6 8RF, United KingdomDepartment of Neuroinflammation, Division of Neuroscience and Psychological Medicine, Imperial College School of Medicine, Charing Cross Hospital, London, W6 8RF, United KingdomWe have studied the neurotoxicity of amyloid-beta (Aβ) after a single unilateral intravitreal injection. Within the retina apoptotic cells were seen throughout the photoreceptor layer and the inner nuclear layer but not in the ganglion cell layer at 48 h after injection of Aβ1-42 compared to vehicle control and control peptide. At 5 months, there was a significant reduction in total cell numbers in the ganglion cell layer in Nissl stained retinas. There was glial cell dysfunction with upregulation of glial fibrillary acidic protein and a reduction in the expression of Müller cell associated proteins in the injected retinas. These results suggest an indirect cytotoxic effect of Aβ on retinal neurons and an important role for dysfunction of Müller glia in mediating Aβ neurotoxicity.http://www.sciencedirect.com/science/article/pii/S0969996102904857amyloid-betaneurotoxicityretinal neuronsMüller cellsglial dysfunctionneurodegeneration |
| spellingShingle | Desmond T. Walsh Rosa M. Montero Laura G. Bresciani Angela Y.T. Jen Pascale D. Leclercq Debbie Saunders Ahmed N. EL-Amir Lukemann Gbadamoshi Stephen M. Gentleman Ling-Sun Jen Amyloid-Beta Peptide Is Toxic to Neurons In Vivo via Indirect Mechanisms Neurobiology of Disease amyloid-beta neurotoxicity retinal neurons Müller cells glial dysfunction neurodegeneration |
| title | Amyloid-Beta Peptide Is Toxic to Neurons In Vivo via Indirect Mechanisms |
| title_full | Amyloid-Beta Peptide Is Toxic to Neurons In Vivo via Indirect Mechanisms |
| title_fullStr | Amyloid-Beta Peptide Is Toxic to Neurons In Vivo via Indirect Mechanisms |
| title_full_unstemmed | Amyloid-Beta Peptide Is Toxic to Neurons In Vivo via Indirect Mechanisms |
| title_short | Amyloid-Beta Peptide Is Toxic to Neurons In Vivo via Indirect Mechanisms |
| title_sort | amyloid beta peptide is toxic to neurons in vivo via indirect mechanisms |
| topic | amyloid-beta neurotoxicity retinal neurons Müller cells glial dysfunction neurodegeneration |
| url | http://www.sciencedirect.com/science/article/pii/S0969996102904857 |
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