MicroRNA-193a inhibits breast cancer proliferation and metastasis by downregulating WT1.
In many cancers, microRNA-193a (miR-193a) is a suppressor miRNA, but its underlying anti-oncogenic activity in breast cancer is not known. In this study, we found decreased miR-193a (specifically, miR-193a-5p) expression not only in breast cancer cell lines but also in breast cancer tissues as compa...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5634539?pdf=render |
| _version_ | 1818305519137325056 |
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| author | FeiYan Xie Sumayyah Hosany Shen Zhong Yang Jiang Fen Zhang LiLi Lin XiaoBo Wang ShenMeng Gao XiaoQu Hu |
| author_facet | FeiYan Xie Sumayyah Hosany Shen Zhong Yang Jiang Fen Zhang LiLi Lin XiaoBo Wang ShenMeng Gao XiaoQu Hu |
| author_sort | FeiYan Xie |
| collection | DOAJ |
| description | In many cancers, microRNA-193a (miR-193a) is a suppressor miRNA, but its underlying anti-oncogenic activity in breast cancer is not known. In this study, we found decreased miR-193a (specifically, miR-193a-5p) expression not only in breast cancer cell lines but also in breast cancer tissues as compared with the adjacent non-tumor tissues. Ectopic miR-193a overexpression inhibited the proliferation, colony formation, migration, and invasion of MDA-MB-231 and BT549 cells. miR-193a reduced Wilms' tumor 1 (WT1) expression and repressed luciferase reporter activity by binding WT1 coding region sequences; mutation of the predicted miR-193a binding site abolished this effect. miR-193a and WT1 expression were significantly inversely correlated in breast cancer tissues. Importantly, the anti-cancer activity induced by miR-193a was partially reversed by WT1 overexpression, indicating an important role for WT1 in such activity related to miR-193a. Our results reveal that miR-193a-WT1 interaction plays an important role in breast cancer metastasis, and suggest that restoring miR-193a expression is a therapeutic strategy in breast cancer. |
| first_indexed | 2024-12-13T06:27:52Z |
| format | Article |
| id | doaj.art-3a8d5a47b91f45fcaf1ed8438eaa452e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T06:27:52Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3a8d5a47b91f45fcaf1ed8438eaa452e2022-12-21T23:56:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011210e018556510.1371/journal.pone.0185565MicroRNA-193a inhibits breast cancer proliferation and metastasis by downregulating WT1.FeiYan XieSumayyah HosanyShen ZhongYang JiangFen ZhangLiLi LinXiaoBo WangShenMeng GaoXiaoQu HuIn many cancers, microRNA-193a (miR-193a) is a suppressor miRNA, but its underlying anti-oncogenic activity in breast cancer is not known. In this study, we found decreased miR-193a (specifically, miR-193a-5p) expression not only in breast cancer cell lines but also in breast cancer tissues as compared with the adjacent non-tumor tissues. Ectopic miR-193a overexpression inhibited the proliferation, colony formation, migration, and invasion of MDA-MB-231 and BT549 cells. miR-193a reduced Wilms' tumor 1 (WT1) expression and repressed luciferase reporter activity by binding WT1 coding region sequences; mutation of the predicted miR-193a binding site abolished this effect. miR-193a and WT1 expression were significantly inversely correlated in breast cancer tissues. Importantly, the anti-cancer activity induced by miR-193a was partially reversed by WT1 overexpression, indicating an important role for WT1 in such activity related to miR-193a. Our results reveal that miR-193a-WT1 interaction plays an important role in breast cancer metastasis, and suggest that restoring miR-193a expression is a therapeutic strategy in breast cancer.http://europepmc.org/articles/PMC5634539?pdf=render |
| spellingShingle | FeiYan Xie Sumayyah Hosany Shen Zhong Yang Jiang Fen Zhang LiLi Lin XiaoBo Wang ShenMeng Gao XiaoQu Hu MicroRNA-193a inhibits breast cancer proliferation and metastasis by downregulating WT1. PLoS ONE |
| title | MicroRNA-193a inhibits breast cancer proliferation and metastasis by downregulating WT1. |
| title_full | MicroRNA-193a inhibits breast cancer proliferation and metastasis by downregulating WT1. |
| title_fullStr | MicroRNA-193a inhibits breast cancer proliferation and metastasis by downregulating WT1. |
| title_full_unstemmed | MicroRNA-193a inhibits breast cancer proliferation and metastasis by downregulating WT1. |
| title_short | MicroRNA-193a inhibits breast cancer proliferation and metastasis by downregulating WT1. |
| title_sort | microrna 193a inhibits breast cancer proliferation and metastasis by downregulating wt1 |
| url | http://europepmc.org/articles/PMC5634539?pdf=render |
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