Dynamic Bioreactor Culture for Infiltration of Bone Mesenchymal Stem Cells within Electrospun Nanofibrous Scaffolds for Annulus Fibrosus Repair
Objective To compare the ability of three culture strategies of static culture, intermittent centrifugal culture and dynamic bioreactor culture in promoting the infiltration of bone marrow mesenchymal stem cells (BMSCs) throughout electrospun nanoporous aligned nanoyarn scaffold (AYS). Methods AYS w...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-02-01
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| Series: | Orthopaedic Surgery |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/os.12615 |
| _version_ | 1818612241345282048 |
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| author | Shuang Wang Yun‐Fei He Jun Ma Lei Yu Jian‐Kun Wen Xiao‐Jian Ye |
| author_facet | Shuang Wang Yun‐Fei He Jun Ma Lei Yu Jian‐Kun Wen Xiao‐Jian Ye |
| author_sort | Shuang Wang |
| collection | DOAJ |
| description | Objective To compare the ability of three culture strategies of static culture, intermittent centrifugal culture and dynamic bioreactor culture in promoting the infiltration of bone marrow mesenchymal stem cells (BMSCs) throughout electrospun nanoporous aligned nanoyarn scaffold (AYS). Methods AYS was constructed by the method of conjugated electrospinning, using the blended solution of poly (L‐lactide‐co‐caprolactone) (P (LLA‐CL)) and gelatin. Then the bone marrow mesenchymal stem cells (BMSCs) were transplanted on the scaffolds. Culture the scaffold‐cells using three methods of static culture, intermittent centrifugal culture and dynamic bioreactor culture. After 7 and 14 days in culture, the infiltration depth of the cells were observed and measured by hematoxylin and eosin (HE) or 4′, 6‐diamidino‐2‐phenylindole (DAPI) staining. Result In the current study, on the 7th day, the BMSCs in the scaffolds of static culture group, intermittent centrifugal culture group, and dynamic bioreactor culture group infiltrated to an average depth of 11.88 ± 1.82 μm, 21.17 ± 13.17 μm, and 26.27 ± 7.42 μm, respectively. There were differences between the bioreactor culture group with the static culture group and the intermittent centrifugal culture group. On the time point of 14 days, the depth of infiltration of BMSCs in dynamic bioreactor culture was the most (115.13 ± 25.44 μm, P < 0.05), and the infiltration of the cells in the intermittent centrifugal culture group was 42.53 ± 13.07 μm, deeper than that of the static culture group (24.53 ± 6.06, P < 0.05). Conclusion Dynamic bioreactor culture may be a preferred method for tissue engineering approaches involving scaffolds with a low porosity, such as those needed for repair of the annulus fibrosus (AF). |
| first_indexed | 2024-12-16T15:43:06Z |
| format | Article |
| id | doaj.art-3a8ebe3260e240f98787143482c2dc11 |
| institution | Directory Open Access Journal |
| issn | 1757-7853 1757-7861 |
| language | English |
| last_indexed | 2024-12-16T15:43:06Z |
| publishDate | 2020-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Orthopaedic Surgery |
| spelling | doaj.art-3a8ebe3260e240f98787143482c2dc112022-12-21T22:25:55ZengWileyOrthopaedic Surgery1757-78531757-78612020-02-0112130431110.1111/os.12615Dynamic Bioreactor Culture for Infiltration of Bone Mesenchymal Stem Cells within Electrospun Nanofibrous Scaffolds for Annulus Fibrosus RepairShuang Wang0Yun‐Fei He1Jun Ma2Lei Yu3Jian‐Kun Wen4Xiao‐Jian Ye5Department of Spinal Surgery, Changzheng Hospital Second Military Medical University Shanghai ChinaDepartment of Orthopedics The 940th Hospital of Joint Logistics Support Force of the Chinese People's Liberation Army Lanzhou ChinaDepartment of Orthopedics Central Theater Command General Hospital of the Chinese People's Liberation Army Wuhan ChinaDepartment of Spinal Surgery, Changzheng Hospital Second Military Medical University Shanghai ChinaDepartment of Spinal Surgery, Changzheng Hospital Second Military Medical University Shanghai ChinaDepartment of Spinal Surgery, Changzheng Hospital Second Military Medical University Shanghai ChinaObjective To compare the ability of three culture strategies of static culture, intermittent centrifugal culture and dynamic bioreactor culture in promoting the infiltration of bone marrow mesenchymal stem cells (BMSCs) throughout electrospun nanoporous aligned nanoyarn scaffold (AYS). Methods AYS was constructed by the method of conjugated electrospinning, using the blended solution of poly (L‐lactide‐co‐caprolactone) (P (LLA‐CL)) and gelatin. Then the bone marrow mesenchymal stem cells (BMSCs) were transplanted on the scaffolds. Culture the scaffold‐cells using three methods of static culture, intermittent centrifugal culture and dynamic bioreactor culture. After 7 and 14 days in culture, the infiltration depth of the cells were observed and measured by hematoxylin and eosin (HE) or 4′, 6‐diamidino‐2‐phenylindole (DAPI) staining. Result In the current study, on the 7th day, the BMSCs in the scaffolds of static culture group, intermittent centrifugal culture group, and dynamic bioreactor culture group infiltrated to an average depth of 11.88 ± 1.82 μm, 21.17 ± 13.17 μm, and 26.27 ± 7.42 μm, respectively. There were differences between the bioreactor culture group with the static culture group and the intermittent centrifugal culture group. On the time point of 14 days, the depth of infiltration of BMSCs in dynamic bioreactor culture was the most (115.13 ± 25.44 μm, P < 0.05), and the infiltration of the cells in the intermittent centrifugal culture group was 42.53 ± 13.07 μm, deeper than that of the static culture group (24.53 ± 6.06, P < 0.05). Conclusion Dynamic bioreactor culture may be a preferred method for tissue engineering approaches involving scaffolds with a low porosity, such as those needed for repair of the annulus fibrosus (AF).https://doi.org/10.1111/os.12615Annulus fibrosusCell culture techniquesMesenchymal stem cellsTissue engineeringTissue scaffolds |
| spellingShingle | Shuang Wang Yun‐Fei He Jun Ma Lei Yu Jian‐Kun Wen Xiao‐Jian Ye Dynamic Bioreactor Culture for Infiltration of Bone Mesenchymal Stem Cells within Electrospun Nanofibrous Scaffolds for Annulus Fibrosus Repair Orthopaedic Surgery Annulus fibrosus Cell culture techniques Mesenchymal stem cells Tissue engineering Tissue scaffolds |
| title | Dynamic Bioreactor Culture for Infiltration of Bone Mesenchymal Stem Cells within Electrospun Nanofibrous Scaffolds for Annulus Fibrosus Repair |
| title_full | Dynamic Bioreactor Culture for Infiltration of Bone Mesenchymal Stem Cells within Electrospun Nanofibrous Scaffolds for Annulus Fibrosus Repair |
| title_fullStr | Dynamic Bioreactor Culture for Infiltration of Bone Mesenchymal Stem Cells within Electrospun Nanofibrous Scaffolds for Annulus Fibrosus Repair |
| title_full_unstemmed | Dynamic Bioreactor Culture for Infiltration of Bone Mesenchymal Stem Cells within Electrospun Nanofibrous Scaffolds for Annulus Fibrosus Repair |
| title_short | Dynamic Bioreactor Culture for Infiltration of Bone Mesenchymal Stem Cells within Electrospun Nanofibrous Scaffolds for Annulus Fibrosus Repair |
| title_sort | dynamic bioreactor culture for infiltration of bone mesenchymal stem cells within electrospun nanofibrous scaffolds for annulus fibrosus repair |
| topic | Annulus fibrosus Cell culture techniques Mesenchymal stem cells Tissue engineering Tissue scaffolds |
| url | https://doi.org/10.1111/os.12615 |
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