Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle
Abstract Given its limited accessibility, the CA2 area has been less investigated compared to other subregions of the hippocampus. While the development of transgenic mice expressing Cre recombinase in the CA2 has revealed unique features of this area, the use of mouse lines has several limitations,...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-27372-8 |
| _version_ | 1797958700511526912 |
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| author | Kazuki Okamoto Yuji Kamikubo Kenta Yamauchi Shinichiro Okamoto Megumu Takahashi Yoko Ishida Masato Koike Yuji Ikegaya Takashi Sakurai Hiroyuki Hioki |
| author_facet | Kazuki Okamoto Yuji Kamikubo Kenta Yamauchi Shinichiro Okamoto Megumu Takahashi Yoko Ishida Masato Koike Yuji Ikegaya Takashi Sakurai Hiroyuki Hioki |
| author_sort | Kazuki Okamoto |
| collection | DOAJ |
| description | Abstract Given its limited accessibility, the CA2 area has been less investigated compared to other subregions of the hippocampus. While the development of transgenic mice expressing Cre recombinase in the CA2 has revealed unique features of this area, the use of mouse lines has several limitations, such as lack of specificity. Therefore, a specific gene delivery system is required. Here, we confirmed that the AAV-PHP.eB capsid preferably infected CA2 pyramidal cells following retro-orbital injection and demonstrated that the specificity was substantially higher after injection into the lateral ventricle. In addition, a tropism for the CA2 area was observed in organotypic slice cultures. Combined injection into the lateral ventricle and stereotaxic injection into the CA2 area specifically introduced the transgene into CA2 pyramidal cells, enabling us to perform targeted patch-clamp recordings and optogenetic manipulation. These results suggest that AAV-PHP.eB is a versatile tool for specific gene transduction in CA2 pyramidal cells. |
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| format | Article |
| id | doaj.art-3a8f624fb84e4f839b03ebdf53eba82f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T00:22:43Z |
| publishDate | 2023-01-01 |
| publisher | Nature Portfolio |
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| spelling | doaj.art-3a8f624fb84e4f839b03ebdf53eba82f2023-01-08T12:09:36ZengNature PortfolioScientific Reports2045-23222023-01-0113111110.1038/s41598-022-27372-8Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricleKazuki Okamoto0Yuji Kamikubo1Kenta Yamauchi2Shinichiro Okamoto3Megumu Takahashi4Yoko Ishida5Masato Koike6Yuji Ikegaya7Takashi Sakurai8Hiroyuki Hioki9Department of Neuroanatomy, Juntendo University Graduate School of MedicineDepartment of Cellular and Molecular Pharmacology, Juntendo University Graduate School of MedicineDepartment of Neuroanatomy, Juntendo University Graduate School of MedicineDepartment of Neuroanatomy, Juntendo University Graduate School of MedicineDepartment of Neuroanatomy, Juntendo University Graduate School of MedicineDepartment of Neuroanatomy, Juntendo University Graduate School of MedicineDepartment of Cell Biology and Neuroscience, Juntendo University Graduate School of MedicineLaboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of TokyoDepartment of Cellular and Molecular Pharmacology, Juntendo University Graduate School of MedicineDepartment of Neuroanatomy, Juntendo University Graduate School of MedicineAbstract Given its limited accessibility, the CA2 area has been less investigated compared to other subregions of the hippocampus. While the development of transgenic mice expressing Cre recombinase in the CA2 has revealed unique features of this area, the use of mouse lines has several limitations, such as lack of specificity. Therefore, a specific gene delivery system is required. Here, we confirmed that the AAV-PHP.eB capsid preferably infected CA2 pyramidal cells following retro-orbital injection and demonstrated that the specificity was substantially higher after injection into the lateral ventricle. In addition, a tropism for the CA2 area was observed in organotypic slice cultures. Combined injection into the lateral ventricle and stereotaxic injection into the CA2 area specifically introduced the transgene into CA2 pyramidal cells, enabling us to perform targeted patch-clamp recordings and optogenetic manipulation. These results suggest that AAV-PHP.eB is a versatile tool for specific gene transduction in CA2 pyramidal cells.https://doi.org/10.1038/s41598-022-27372-8 |
| spellingShingle | Kazuki Okamoto Yuji Kamikubo Kenta Yamauchi Shinichiro Okamoto Megumu Takahashi Yoko Ishida Masato Koike Yuji Ikegaya Takashi Sakurai Hiroyuki Hioki Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle Scientific Reports |
| title | Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle |
| title_full | Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle |
| title_fullStr | Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle |
| title_full_unstemmed | Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle |
| title_short | Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle |
| title_sort | specific aav2 php eb mediated gene transduction of ca2 pyramidal cells via injection into the lateral ventricle |
| url | https://doi.org/10.1038/s41598-022-27372-8 |
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