Effects of spinetoram and glyphosate on physiological biomarkers and gut microbes in Bombus terrestris
The sublethal effects of pesticide poisoning will have significant negative impacts on the foraging and learning of bees and bumblebees, so it has received widespread attention. However, little is known about the physiological effects of sublethal spinetoram and glyphosate exposure on bumblebees. We...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-01-01
|
| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.1054742/full |
| _version_ | 1797957588792377344 |
|---|---|
| author | Qi-He Tang Qi-He Tang Wan-Li Li Jie-Ping Wang Xi-Jie Li Dan Li Zhe Cao Qi Huang Jia-Li Li Jun Zhang Zheng-Wei Wang Jun Guo Ji-Lian Li |
| author_facet | Qi-He Tang Qi-He Tang Wan-Li Li Jie-Ping Wang Xi-Jie Li Dan Li Zhe Cao Qi Huang Jia-Li Li Jun Zhang Zheng-Wei Wang Jun Guo Ji-Lian Li |
| author_sort | Qi-He Tang |
| collection | DOAJ |
| description | The sublethal effects of pesticide poisoning will have significant negative impacts on the foraging and learning of bees and bumblebees, so it has received widespread attention. However, little is known about the physiological effects of sublethal spinetoram and glyphosate exposure on bumblebees. We continuously exposed Bombus terrestris to sublethal (2.5 mg/L) spinetoram or glyphosate under controlled conditions for 10 days. The superoxide dismutase, glutathione-S-transferase, carboxylesterase, prophenoloxidase, α-amylase and protease activities, and changes in gut microbes were measured to understand the effects of sublethal pesticide exposure on the physiology and gut microbes of bumblebees. Sublethal pesticide exposure to significantly increased superoxide dismutase activity and significantly decreased gut α-amylase activity in bumblebees but had no significant effect on glutathione-S-transferase, carboxylesterase or gut protease activities. In addition, glyphosate increased the activity of prophenoloxidase. Interestingly, we observed that neither of the two pesticides had a significant effect on dominant gut bacteria, but glyphosate significantly altered the structure of the dominant gut fungal community, and reduced the relative abundance of Zygosaccharomyces associated with fat accumulation. These results suggest that sublethal spinetoram and glyphosate do not significantly affect the detoxification system of bumblebees, but may affect bumblebee health by inhibiting energy acquisition. Our results provide information on the sublethal effects of exposure to low concentrations of glyphosate and spinetoram on bumblebees in terms of physiology and gut microbes. |
| first_indexed | 2024-04-11T00:07:29Z |
| format | Article |
| id | doaj.art-3a91a1e77f8747a8b69814da1d8cf531 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-11T00:07:29Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-3a91a1e77f8747a8b69814da1d8cf5312023-01-09T09:58:10ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-01-011310.3389/fphys.2022.10547421054742Effects of spinetoram and glyphosate on physiological biomarkers and gut microbes in Bombus terrestrisQi-He Tang0Qi-He Tang1Wan-Li Li2Jie-Ping Wang3Xi-Jie Li4Dan Li5Zhe Cao6Qi Huang7Jia-Li Li8Jun Zhang9Zheng-Wei Wang10Jun Guo11Ji-Lian Li12Key Laboratory for Insect-Pollinator Biology, Ministry of Agriculture and Rural Affairs, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaChongQing Academy of Animal Sciences, Chongqing, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaCAS Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Jinghong, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, ChinaKey Laboratory for Insect-Pollinator Biology, Ministry of Agriculture and Rural Affairs, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, ChinaThe sublethal effects of pesticide poisoning will have significant negative impacts on the foraging and learning of bees and bumblebees, so it has received widespread attention. However, little is known about the physiological effects of sublethal spinetoram and glyphosate exposure on bumblebees. We continuously exposed Bombus terrestris to sublethal (2.5 mg/L) spinetoram or glyphosate under controlled conditions for 10 days. The superoxide dismutase, glutathione-S-transferase, carboxylesterase, prophenoloxidase, α-amylase and protease activities, and changes in gut microbes were measured to understand the effects of sublethal pesticide exposure on the physiology and gut microbes of bumblebees. Sublethal pesticide exposure to significantly increased superoxide dismutase activity and significantly decreased gut α-amylase activity in bumblebees but had no significant effect on glutathione-S-transferase, carboxylesterase or gut protease activities. In addition, glyphosate increased the activity of prophenoloxidase. Interestingly, we observed that neither of the two pesticides had a significant effect on dominant gut bacteria, but glyphosate significantly altered the structure of the dominant gut fungal community, and reduced the relative abundance of Zygosaccharomyces associated with fat accumulation. These results suggest that sublethal spinetoram and glyphosate do not significantly affect the detoxification system of bumblebees, but may affect bumblebee health by inhibiting energy acquisition. Our results provide information on the sublethal effects of exposure to low concentrations of glyphosate and spinetoram on bumblebees in terms of physiology and gut microbes.https://www.frontiersin.org/articles/10.3389/fphys.2022.1054742/fullbumblebeesglyphosate (GLY)spinetoramsublethal effectsgut microbesphysiological biomarkers |
| spellingShingle | Qi-He Tang Qi-He Tang Wan-Li Li Jie-Ping Wang Xi-Jie Li Dan Li Zhe Cao Qi Huang Jia-Li Li Jun Zhang Zheng-Wei Wang Jun Guo Ji-Lian Li Effects of spinetoram and glyphosate on physiological biomarkers and gut microbes in Bombus terrestris Frontiers in Physiology bumblebees glyphosate (GLY) spinetoram sublethal effects gut microbes physiological biomarkers |
| title | Effects of spinetoram and glyphosate on physiological biomarkers and gut microbes in Bombus terrestris |
| title_full | Effects of spinetoram and glyphosate on physiological biomarkers and gut microbes in Bombus terrestris |
| title_fullStr | Effects of spinetoram and glyphosate on physiological biomarkers and gut microbes in Bombus terrestris |
| title_full_unstemmed | Effects of spinetoram and glyphosate on physiological biomarkers and gut microbes in Bombus terrestris |
| title_short | Effects of spinetoram and glyphosate on physiological biomarkers and gut microbes in Bombus terrestris |
| title_sort | effects of spinetoram and glyphosate on physiological biomarkers and gut microbes in bombus terrestris |
| topic | bumblebees glyphosate (GLY) spinetoram sublethal effects gut microbes physiological biomarkers |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.1054742/full |
| work_keys_str_mv | AT qihetang effectsofspinetoramandglyphosateonphysiologicalbiomarkersandgutmicrobesinbombusterrestris AT qihetang effectsofspinetoramandglyphosateonphysiologicalbiomarkersandgutmicrobesinbombusterrestris AT wanlili effectsofspinetoramandglyphosateonphysiologicalbiomarkersandgutmicrobesinbombusterrestris AT jiepingwang effectsofspinetoramandglyphosateonphysiologicalbiomarkersandgutmicrobesinbombusterrestris AT xijieli effectsofspinetoramandglyphosateonphysiologicalbiomarkersandgutmicrobesinbombusterrestris AT danli effectsofspinetoramandglyphosateonphysiologicalbiomarkersandgutmicrobesinbombusterrestris AT zhecao effectsofspinetoramandglyphosateonphysiologicalbiomarkersandgutmicrobesinbombusterrestris AT qihuang effectsofspinetoramandglyphosateonphysiologicalbiomarkersandgutmicrobesinbombusterrestris AT jialili effectsofspinetoramandglyphosateonphysiologicalbiomarkersandgutmicrobesinbombusterrestris AT junzhang effectsofspinetoramandglyphosateonphysiologicalbiomarkersandgutmicrobesinbombusterrestris AT zhengweiwang effectsofspinetoramandglyphosateonphysiologicalbiomarkersandgutmicrobesinbombusterrestris AT junguo effectsofspinetoramandglyphosateonphysiologicalbiomarkersandgutmicrobesinbombusterrestris AT jilianli effectsofspinetoramandglyphosateonphysiologicalbiomarkersandgutmicrobesinbombusterrestris |