The in vivo antioxidant effect of vitamin C on hemogram in Paraquat treated male rats (rattus norvegicus)
Paraquat (PQ) is one of the most used herbicide globally; applied around trees in orchards and between crop rows to control broad-leaved and grassy weeds. Its oxidation results in the formation of superoxides which causes damage to cellular components. In this study, we determined the antioxidant ef...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-06-01
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| Series: | Journal of Xenobiotics |
| Subjects: | |
| Online Access: | http://www.pagepressjournals.org/index.php/xeno/article/view/1161 |
| Summary: | Paraquat (PQ) is one of the most used herbicide globally; applied around trees in orchards and between crop rows to control broad-leaved and grassy weeds. Its oxidation results in the formation of superoxides which causes damage to cellular components. In this study, we determined the antioxidant effect vitamin C has on hemograms [hemoglobin (Hb), packed cell volume (PCV) and total white blood cells count] of rats under these toxic insults. The animals grouped (A-D), comprising subgroups without vitamin C (A<sub>1</sub>, B<sub>1</sub>, C<sub>1</sub>, D<sub>1</sub>) and subgroups on vitamin C (A<sub>2</sub>, B<sub>2</sub>, C<sub>2</sub>, D<sub>2</sub>), received different sub-lethal doses of PQ administered intraperitoneally monthly to the animals over a period of three months. The Hb values obtained were significantly reduced (P≤0.05) at month 1 and (P≤0.001) at months 2 and 3. These changes became more pronounced with increased dose and time. Vitamin C treated subgroups (B<sub>2</sub>, C<sub>2</sub> and D<sub>2</sub>) had better Hb values than those without it (B<sub>1</sub>, C<sub>1</sub> and D<sub>1</sub>) but the values were still significantly low when compared to the control subgroups (A<sub>1</sub> and A<sub>2</sub>). This same trend was observed in the PCV results obtained. The Control subgroups showed that vitamin C treated subgroup (A<sub>2</sub>) had a more improved hemogram values than subgroup on water only (A<sub>1</sub>), but they were all higher than that of the test subgroups. These PQ induced anaemia were ameliorated by the subsequent administration of vitamin C, and continuous treatment with vitamin C restored the health status of the animals so treated. |
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| ISSN: | 2039-4705 2039-4713 |