Differential Expression of LncRNA in Bladder Cancer Development
Bladder cancer (BC) is the tenth most common cancer, with urothelial carcinoma representing about 90% of all BC, including neoplasms and carcinomas of different grades of malignancy. Urinary cytology has a significant role in BC screening and surveillance, although it has a low detection rate and hi...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/13/10/1745 |
| _version_ | 1797600392798797824 |
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| author | Lorenzo Spirito Rufina Maturi Sara Carmela Credendino Celeste Manfredi Davide Arcaniolo Marco De Martino Francesco Esposito Luigi Napolitano Francesco Di Bello Alfredo Fusco Pierlorenzo Pallante Marco De Sio Gabriella De Vita |
| author_facet | Lorenzo Spirito Rufina Maturi Sara Carmela Credendino Celeste Manfredi Davide Arcaniolo Marco De Martino Francesco Esposito Luigi Napolitano Francesco Di Bello Alfredo Fusco Pierlorenzo Pallante Marco De Sio Gabriella De Vita |
| author_sort | Lorenzo Spirito |
| collection | DOAJ |
| description | Bladder cancer (BC) is the tenth most common cancer, with urothelial carcinoma representing about 90% of all BC, including neoplasms and carcinomas of different grades of malignancy. Urinary cytology has a significant role in BC screening and surveillance, although it has a low detection rate and high dependence on the pathologist’s experience. The currently available biomarkers are not implemented into routine clinical practice due to high costs or low sensitivity. In recent years, the role of lncRNAs in BC has emerged, even though it is still poorly explored. We have previously shown that the lncRNAs Metallophosphoesterase Domain-Containing 2 Antisense RNA 1 (MPPED2-AS1), Rhabdomyosarcoma-2 Associated Transcript (RMST), Kelch-like protein 14 antisense (Klhl14AS) and Prader Willi/Angelman region RNA 5 (PAR5) are involved in the progression of different types of cancers. Here, we investigated the expression of these molecules in BC, first by interrogating the GEPIA database and observing a different distribution of expression levels between normal and cancer specimens. We then measured them in a cohort of neoplastic bladder lesions, either benign or malignant, from patients with suspicion of BC undergoing transurethral resection of bladder tumor (TURBT). The total RNA from biopsies was analyzed using qRT-PCR for the expression of the four lncRNA genes, showing differential expression of the investigated lncRNAs between normal tissue, benign lesions and cancers. In conclusion, the data reported here highlight the involvement of novel lncRNAs in BC development, whose altered expression could potentially affect the regulatory circuits in which these molecules are involved. Our study paves the way for testing lncRNA genes as markers for BC diagnosis and/or follow-up. |
| first_indexed | 2024-03-11T03:47:29Z |
| format | Article |
| id | doaj.art-3a97c8fdf9da4dec85b54ca83f5f3420 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-11T03:47:29Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-3a97c8fdf9da4dec85b54ca83f5f34202023-11-18T01:04:31ZengMDPI AGDiagnostics2075-44182023-05-011310174510.3390/diagnostics13101745Differential Expression of LncRNA in Bladder Cancer DevelopmentLorenzo Spirito0Rufina Maturi1Sara Carmela Credendino2Celeste Manfredi3Davide Arcaniolo4Marco De Martino5Francesco Esposito6Luigi Napolitano7Francesco Di Bello8Alfredo Fusco9Pierlorenzo Pallante10Marco De Sio11Gabriella De Vita12Urology Unit, Department of Woman Child and General and Specialized Surgery, University of Campania “Luigi Vanvitelli”, Via De Crecchio 7, 80138 Naples, ItalyDepartment of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via Pansini 5, 80131 Naples, ItalyDepartment of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via Pansini 5, 80131 Naples, ItalyUrology Unit, Department of Woman Child and General and Specialized Surgery, University of Campania “Luigi Vanvitelli”, Via De Crecchio 7, 80138 Naples, ItalyUrology Unit, Department of Woman Child and General and Specialized Surgery, University of Campania “Luigi Vanvitelli”, Via De Crecchio 7, 80138 Naples, ItalyInstitute of Experimental Endocrinology and Oncology “G. Salvatore”, National Research Council (CNR), Via Pansini, 5, 80131 Naples, ItalyInstitute of Experimental Endocrinology and Oncology “G. Salvatore”, National Research Council (CNR), Via Pansini, 5, 80131 Naples, ItalyUrology Unit, Department of Neurosciences, Reproductive Sciences, and Odontostomatology, University of Naples Federico II, Via Pansini 5, 80131 Naples, ItalyUrology Unit, Department of Neurosciences, Reproductive Sciences, and Odontostomatology, University of Naples Federico II, Via Pansini 5, 80131 Naples, ItalyDepartment of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via Pansini 5, 80131 Naples, ItalyInstitute of Experimental Endocrinology and Oncology “G. Salvatore”, National Research Council (CNR), Via Pansini, 5, 80131 Naples, ItalyUrology Unit, Department of Woman Child and General and Specialized Surgery, University of Campania “Luigi Vanvitelli”, Via De Crecchio 7, 80138 Naples, ItalyDepartment of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via Pansini 5, 80131 Naples, ItalyBladder cancer (BC) is the tenth most common cancer, with urothelial carcinoma representing about 90% of all BC, including neoplasms and carcinomas of different grades of malignancy. Urinary cytology has a significant role in BC screening and surveillance, although it has a low detection rate and high dependence on the pathologist’s experience. The currently available biomarkers are not implemented into routine clinical practice due to high costs or low sensitivity. In recent years, the role of lncRNAs in BC has emerged, even though it is still poorly explored. We have previously shown that the lncRNAs Metallophosphoesterase Domain-Containing 2 Antisense RNA 1 (MPPED2-AS1), Rhabdomyosarcoma-2 Associated Transcript (RMST), Kelch-like protein 14 antisense (Klhl14AS) and Prader Willi/Angelman region RNA 5 (PAR5) are involved in the progression of different types of cancers. Here, we investigated the expression of these molecules in BC, first by interrogating the GEPIA database and observing a different distribution of expression levels between normal and cancer specimens. We then measured them in a cohort of neoplastic bladder lesions, either benign or malignant, from patients with suspicion of BC undergoing transurethral resection of bladder tumor (TURBT). The total RNA from biopsies was analyzed using qRT-PCR for the expression of the four lncRNA genes, showing differential expression of the investigated lncRNAs between normal tissue, benign lesions and cancers. In conclusion, the data reported here highlight the involvement of novel lncRNAs in BC development, whose altered expression could potentially affect the regulatory circuits in which these molecules are involved. Our study paves the way for testing lncRNA genes as markers for BC diagnosis and/or follow-up.https://www.mdpi.com/2075-4418/13/10/1745bladder cancerlncRNAsgene expression |
| spellingShingle | Lorenzo Spirito Rufina Maturi Sara Carmela Credendino Celeste Manfredi Davide Arcaniolo Marco De Martino Francesco Esposito Luigi Napolitano Francesco Di Bello Alfredo Fusco Pierlorenzo Pallante Marco De Sio Gabriella De Vita Differential Expression of LncRNA in Bladder Cancer Development Diagnostics bladder cancer lncRNAs gene expression |
| title | Differential Expression of LncRNA in Bladder Cancer Development |
| title_full | Differential Expression of LncRNA in Bladder Cancer Development |
| title_fullStr | Differential Expression of LncRNA in Bladder Cancer Development |
| title_full_unstemmed | Differential Expression of LncRNA in Bladder Cancer Development |
| title_short | Differential Expression of LncRNA in Bladder Cancer Development |
| title_sort | differential expression of lncrna in bladder cancer development |
| topic | bladder cancer lncRNAs gene expression |
| url | https://www.mdpi.com/2075-4418/13/10/1745 |
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