Optimal Time to Ship Human Islets Post Tissue Culture to Maximize Islet Recovery
Access to functional high-quality pancreatic human islets is critical to advance diabetes research. The Integrated Islet Distribution Program (IIDP), a major source for human islet distribution for over 15 years, conducted a study to evaluate the most advantageous times to ship islets postisolation...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2020-11-01
|
| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/0963689720974582 |
| _version_ | 1818731331123675136 |
|---|---|
| author | Barbara J. Olack Michael Alexander Carol J. Swanson Julie Kilburn Nicole Corrales Antonio Flores Jennifer Heng Jayagowri Arulmoli Keiko Omori Peter J. Chlebeck Laura Zitur Mayra Salgado Jonathan R.T. Lakey Joyce C. Niland |
| author_facet | Barbara J. Olack Michael Alexander Carol J. Swanson Julie Kilburn Nicole Corrales Antonio Flores Jennifer Heng Jayagowri Arulmoli Keiko Omori Peter J. Chlebeck Laura Zitur Mayra Salgado Jonathan R.T. Lakey Joyce C. Niland |
| author_sort | Barbara J. Olack |
| collection | DOAJ |
| description | Access to functional high-quality pancreatic human islets is critical to advance diabetes research. The Integrated Islet Distribution Program (IIDP), a major source for human islet distribution for over 15 years, conducted a study to evaluate the most advantageous times to ship islets postisolation to maximize islet recovery. For the evaluation, three experienced IIDP Islet Isolation Centers each provided samples from five human islet isolations, shipping 10,000 islet equivalents (IEQ) at four different time periods postislet isolation (no 37°C culture and shipped within 0 to 18 hours; or held in 37°C culture for 18 to 42, 48 to 96, or 144 to 192 hours). A central evaluation center compared samples for islet quantity, quality, and viability for each experimental condition preshipment and postshipment, as well as post 37°C culture 18 to 24 hours after shipment receipt. Additional evaluations included measures of functional potency by static glucose-stimulated insulin release (GSIR), represented as a stimulation index. Comparing the results of the four preshipment holding periods, the greatest IEQ loss postshipment occurred with the shortest preshipment times. Similar patterns emerged when comparing preshipment to postculture losses. In vitro islet function (GSIR) was not adversely impacted by increased tissue culture time. These data indicate that allowing time for islet recovery postisolation, prior to shipping, yields less islet loss during shipment without decreasing islet function. |
| first_indexed | 2024-12-17T23:15:58Z |
| format | Article |
| id | doaj.art-3a982b8a25d949beb6d2b94c00388c94 |
| institution | Directory Open Access Journal |
| issn | 1555-3892 |
| language | English |
| last_indexed | 2024-12-17T23:15:58Z |
| publishDate | 2020-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-3a982b8a25d949beb6d2b94c00388c942022-12-21T21:29:00ZengSAGE PublishingCell Transplantation1555-38922020-11-012910.1177/0963689720974582Optimal Time to Ship Human Islets Post Tissue Culture to Maximize Islet RecoveryBarbara J. Olack0Michael Alexander1Carol J. Swanson2Julie Kilburn3Nicole Corrales4Antonio Flores5Jennifer Heng6Jayagowri Arulmoli7Keiko Omori8Peter J. Chlebeck9Laura Zitur10Mayra Salgado11Jonathan R.T. Lakey12Joyce C. Niland13 Integrated Islet Distribution Program, Department of Diabetes & Cancer Discovery Science, City of Hope, Duarte, CA, USA Department of Surgery, University of California Irvine, Orange, CA, USA Integrated Islet Distribution Program, Department of Diabetes & Cancer Discovery Science, City of Hope, Duarte, CA, USA Integrated Islet Distribution Program, Department of Diabetes & Cancer Discovery Science, City of Hope, Duarte, CA, USA Department of Surgery, University of California Irvine, Orange, CA, USA Department of Surgery, University of California Irvine, Orange, CA, USA Department of Surgery, University of California Irvine, Orange, CA, USA Scharp-Lacy Research Institute, Aliso Viejo, CA, USA Department of Translational Research and Cellular Therapeutics, City of Hope, Duarte, CA, USA Department of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA Department of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA Department of Translational Research and Cellular Therapeutics, City of Hope, Duarte, CA, USA Department of Biomedical Engineering, University of California Irvine, Irvine, CA, USA Integrated Islet Distribution Program, Department of Diabetes & Cancer Discovery Science, City of Hope, Duarte, CA, USAAccess to functional high-quality pancreatic human islets is critical to advance diabetes research. The Integrated Islet Distribution Program (IIDP), a major source for human islet distribution for over 15 years, conducted a study to evaluate the most advantageous times to ship islets postisolation to maximize islet recovery. For the evaluation, three experienced IIDP Islet Isolation Centers each provided samples from five human islet isolations, shipping 10,000 islet equivalents (IEQ) at four different time periods postislet isolation (no 37°C culture and shipped within 0 to 18 hours; or held in 37°C culture for 18 to 42, 48 to 96, or 144 to 192 hours). A central evaluation center compared samples for islet quantity, quality, and viability for each experimental condition preshipment and postshipment, as well as post 37°C culture 18 to 24 hours after shipment receipt. Additional evaluations included measures of functional potency by static glucose-stimulated insulin release (GSIR), represented as a stimulation index. Comparing the results of the four preshipment holding periods, the greatest IEQ loss postshipment occurred with the shortest preshipment times. Similar patterns emerged when comparing preshipment to postculture losses. In vitro islet function (GSIR) was not adversely impacted by increased tissue culture time. These data indicate that allowing time for islet recovery postisolation, prior to shipping, yields less islet loss during shipment without decreasing islet function.https://doi.org/10.1177/0963689720974582 |
| spellingShingle | Barbara J. Olack Michael Alexander Carol J. Swanson Julie Kilburn Nicole Corrales Antonio Flores Jennifer Heng Jayagowri Arulmoli Keiko Omori Peter J. Chlebeck Laura Zitur Mayra Salgado Jonathan R.T. Lakey Joyce C. Niland Optimal Time to Ship Human Islets Post Tissue Culture to Maximize Islet Recovery Cell Transplantation |
| title | Optimal Time to Ship Human Islets Post Tissue Culture to Maximize Islet Recovery |
| title_full | Optimal Time to Ship Human Islets Post Tissue Culture to Maximize Islet Recovery |
| title_fullStr | Optimal Time to Ship Human Islets Post Tissue Culture to Maximize Islet Recovery |
| title_full_unstemmed | Optimal Time to Ship Human Islets Post Tissue Culture to Maximize Islet Recovery |
| title_short | Optimal Time to Ship Human Islets Post Tissue Culture to Maximize Islet Recovery |
| title_sort | optimal time to ship human islets post tissue culture to maximize islet recovery |
| url | https://doi.org/10.1177/0963689720974582 |
| work_keys_str_mv | AT barbarajolack optimaltimetoshiphumanisletsposttissueculturetomaximizeisletrecovery AT michaelalexander optimaltimetoshiphumanisletsposttissueculturetomaximizeisletrecovery AT caroljswanson optimaltimetoshiphumanisletsposttissueculturetomaximizeisletrecovery AT juliekilburn optimaltimetoshiphumanisletsposttissueculturetomaximizeisletrecovery AT nicolecorrales optimaltimetoshiphumanisletsposttissueculturetomaximizeisletrecovery AT antonioflores optimaltimetoshiphumanisletsposttissueculturetomaximizeisletrecovery AT jenniferheng optimaltimetoshiphumanisletsposttissueculturetomaximizeisletrecovery AT jayagowriarulmoli optimaltimetoshiphumanisletsposttissueculturetomaximizeisletrecovery AT keikoomori optimaltimetoshiphumanisletsposttissueculturetomaximizeisletrecovery AT peterjchlebeck optimaltimetoshiphumanisletsposttissueculturetomaximizeisletrecovery AT laurazitur optimaltimetoshiphumanisletsposttissueculturetomaximizeisletrecovery AT mayrasalgado optimaltimetoshiphumanisletsposttissueculturetomaximizeisletrecovery AT jonathanrtlakey optimaltimetoshiphumanisletsposttissueculturetomaximizeisletrecovery AT joycecniland optimaltimetoshiphumanisletsposttissueculturetomaximizeisletrecovery |