Molecular characterization of carbapenem-resistant Acinetobacter baumannii strains from a tertiary care center in South India
Objectives: Carbapenem resistantAcinetobacter baumannii is an important therapeutic and infection control challenge worldwide. In this study, we investigated the prevalence and distribution of molecular mechanisms of resistance among carbapenem resistant A. baumannii species at a tertiary care sett...
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| Format: | Article |
| Language: | English |
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Asociación Colombiana de Infectología
2020-01-01
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| Series: | Infectio |
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| Online Access: | https://www.revistainfectio.org/index.php/infectio/article/view/824 |
| _version_ | 1811267557175328768 |
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| author | Bhuvanesh Sukhlal Kalal |
| author_facet | Bhuvanesh Sukhlal Kalal |
| author_sort | Bhuvanesh Sukhlal Kalal |
| collection | DOAJ |
| description | Objectives: Carbapenem resistantAcinetobacter baumannii is an important therapeutic and infection control challenge worldwide. In this study, we investigated
the prevalence and distribution of molecular mechanisms of resistance among carbapenem resistant A. baumannii species at a tertiary care setting in South India.
Materials and Methods: A total of 89 non-duplicate clinical isolates of carbapenem-resistantA. baumannii were collected from critical care units of St. John’s Medical College Hospital, Bengaluru, India. Polymerase chain reaction (PCR) was performed to detect blaOXA type carbapenemase blaOXA-51-like, blaOXA-23-like, blaOXA-24-like
and blaOXA-58-like, MBL genes blaNDM, blaIMP, and blaVIM genes. Molecular typing of carbapenem-resistant A. baumannii strains was performed by using Rep-PCR.
Results: Eighty-seven of the isolates were found to carry the blaOXA-51 gene and 81 (91%) isolates were found to have blaOXA-51-like gene and blaOXA-23, gene. The
blaOXA-24 like gene was detected in two isolates of which one also carried blaOXA-51 like and one isolate carried blaVIM coding gene. The prevalence of blaNDM, blaIMP,
blaVIM genes was 12(13%),14 (16%) and 57(64%) respectively. Cluster analyses revealed a 90% similarity and were divided into 5 clusters. Most of the isolates containing carbapenemases coding genes grouped under cluster A, C and UC. Considerable heterogeneity was observed within UC cluster indicating circulation of
multiple strains of A. baumannii within our institution.
Conclusions: Carbapenemase coding blaOXA-23, blaOXA-24 and blaOXA-51 -like were more common than blaVIM and blaNDM. The presence of blaNDM with other genes coding for carbapenemases indicate the ability of the strains to acquire novel genes despite having its share of the blaOXA like carbapenemase. |
| first_indexed | 2024-04-12T21:04:39Z |
| format | Article |
| id | doaj.art-3a9a0bb5d5fb409eba97aab2d346c8df |
| institution | Directory Open Access Journal |
| issn | 0123-9392 |
| language | English |
| last_indexed | 2024-04-12T21:04:39Z |
| publishDate | 2020-01-01 |
| publisher | Asociación Colombiana de Infectología |
| record_format | Article |
| series | Infectio |
| spelling | doaj.art-3a9a0bb5d5fb409eba97aab2d346c8df2022-12-22T03:16:44ZengAsociación Colombiana de InfectologíaInfectio0123-93922020-01-01241273410.22354/in.v24i1.824Molecular characterization of carbapenem-resistant Acinetobacter baumannii strains from a tertiary care center in South IndiaBhuvanesh Sukhlal Kalal0A. J. Research Centre, A. J. Institute of Medical Sciences and Research Centre, Mangaluru, India.Objectives: Carbapenem resistantAcinetobacter baumannii is an important therapeutic and infection control challenge worldwide. In this study, we investigated the prevalence and distribution of molecular mechanisms of resistance among carbapenem resistant A. baumannii species at a tertiary care setting in South India. Materials and Methods: A total of 89 non-duplicate clinical isolates of carbapenem-resistantA. baumannii were collected from critical care units of St. John’s Medical College Hospital, Bengaluru, India. Polymerase chain reaction (PCR) was performed to detect blaOXA type carbapenemase blaOXA-51-like, blaOXA-23-like, blaOXA-24-like and blaOXA-58-like, MBL genes blaNDM, blaIMP, and blaVIM genes. Molecular typing of carbapenem-resistant A. baumannii strains was performed by using Rep-PCR. Results: Eighty-seven of the isolates were found to carry the blaOXA-51 gene and 81 (91%) isolates were found to have blaOXA-51-like gene and blaOXA-23, gene. The blaOXA-24 like gene was detected in two isolates of which one also carried blaOXA-51 like and one isolate carried blaVIM coding gene. The prevalence of blaNDM, blaIMP, blaVIM genes was 12(13%),14 (16%) and 57(64%) respectively. Cluster analyses revealed a 90% similarity and were divided into 5 clusters. Most of the isolates containing carbapenemases coding genes grouped under cluster A, C and UC. Considerable heterogeneity was observed within UC cluster indicating circulation of multiple strains of A. baumannii within our institution. Conclusions: Carbapenemase coding blaOXA-23, blaOXA-24 and blaOXA-51 -like were more common than blaVIM and blaNDM. The presence of blaNDM with other genes coding for carbapenemases indicate the ability of the strains to acquire novel genes despite having its share of the blaOXA like carbapenemase.https://www.revistainfectio.org/index.php/infectio/article/view/824acinetobacter baumanniicarbapenemasesgenomic diversityrep-pcrcarbapenem resistance |
| spellingShingle | Bhuvanesh Sukhlal Kalal Molecular characterization of carbapenem-resistant Acinetobacter baumannii strains from a tertiary care center in South India Infectio acinetobacter baumannii carbapenemases genomic diversity rep-pcr carbapenem resistance |
| title | Molecular characterization of carbapenem-resistant Acinetobacter baumannii strains from a tertiary care center in South India |
| title_full | Molecular characterization of carbapenem-resistant Acinetobacter baumannii strains from a tertiary care center in South India |
| title_fullStr | Molecular characterization of carbapenem-resistant Acinetobacter baumannii strains from a tertiary care center in South India |
| title_full_unstemmed | Molecular characterization of carbapenem-resistant Acinetobacter baumannii strains from a tertiary care center in South India |
| title_short | Molecular characterization of carbapenem-resistant Acinetobacter baumannii strains from a tertiary care center in South India |
| title_sort | molecular characterization of carbapenem resistant acinetobacter baumannii strains from a tertiary care center in south india |
| topic | acinetobacter baumannii carbapenemases genomic diversity rep-pcr carbapenem resistance |
| url | https://www.revistainfectio.org/index.php/infectio/article/view/824 |
| work_keys_str_mv | AT bhuvaneshsukhlalkalal molecularcharacterizationofcarbapenemresistantacinetobacterbaumanniistrainsfromatertiarycarecenterinsouthindia |