Evaluation of Neisseria gonorrhoeae Isolates Susceptibility to Tetracycline Antibiotics from 9 Provinces in China Since 2020
Qian Zhou,1,2,* Wenqi Xu,1,2,* Xiaoyu Zhu,1,2 Jin Zhang,1,2 Yan Zhang,1,2 Yan Han,1,2 Kai Chen,1,2 Yueping Yin1,2 1Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, People’s Republic of China; 2STD Reference Laboratory, National...
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Dove Medical Press
2022-03-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/evaluation-of-neisseria-gonorrhoeae-isolates-susceptibility-to-tetracy-peer-reviewed-fulltext-article-IDR |
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| author | Zhou Q Xu W Zhu X Zhang J Zhang Y Han Y Chen K Yin Y |
| author_facet | Zhou Q Xu W Zhu X Zhang J Zhang Y Han Y Chen K Yin Y |
| author_sort | Zhou Q |
| collection | DOAJ |
| description | Qian Zhou,1,2,* Wenqi Xu,1,2,* Xiaoyu Zhu,1,2 Jin Zhang,1,2 Yan Zhang,1,2 Yan Han,1,2 Kai Chen,1,2 Yueping Yin1,2 1Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, People’s Republic of China; 2STD Reference Laboratory, National Center for Sexually Transmitted Diseases Control, Chinese Center for Disease Control and Prevention, Nanjing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yueping Yin, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, People’s Republic of China, Email yinyp@ncstdlc.orgPurpose: The increasing drug resistance of Neisseria gonorrhoeae has become a serious public health concern. This study investigated N. gonorrhoeae isolates susceptibility to tetracycline antibiotics and the correlation between minimum inhibitory concentrations (MICs) of different antibiotics. The presence of resistance determinants in N. gonorrhoeae strains displaying different levels of tigecycline resistance was also compared.Methods: The minimum inhibitory concentrations (MICs) of tetracycline, minocycline, tigecycline, eravacycline, omadacycline on 412 N. gonorrhoeae isolates were measured by the agar dilution method. The MICs of ceftriaxone and azithromycin were also measured to determine the correlations between antibiotics by the value of the correlation coefficient R. The presence of resistance determinants was identified through polymerase chain reaction (PCR) and sequencing.Results: The MIC90 was 64mg/L for tetracycline, 64mg/L for minocycline, 0.5mg/L for tigecycline, 0.5mg/L for eravacycline, 4mg/L for omadacycline, 0.25 mg/L for ceftriaxone, and 1mg/L for azithromycin. The MIC90 and mode of tigecycline and eravacycline were much lower than those of tetracycline and minocycline. A poor correlation between omadacycline, eravacycline and tetracycline susceptibility was observed. Minocycline has a strong correlation with tetracycline. PorB1 typing, TetM-encoding plasmid, and mtrR promoter adenine deletion were significantly correlated with tigecycline MIC > 0.25mg/L.Conclusion: This study suggested that tigecycline and eravacycline had better in vitro activity and might be alternative antibiotics against resistant N. gonorrhoeae infections. Nevertheless, further in vitro experiments and clinical studies are needed for verification.Keywords: Neisseria gonorrhoeae, antibiotics susceptibility, tigecycline, eravacycline, omadacycline |
| first_indexed | 2024-12-13T07:56:23Z |
| format | Article |
| id | doaj.art-3a9d9614bf1e4a57907a34a383064362 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-13T07:56:23Z |
| publishDate | 2022-03-01 |
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| series | Infection and Drug Resistance |
| spelling | doaj.art-3a9d9614bf1e4a57907a34a3830643622022-12-21T23:54:31ZengDove Medical PressInfection and Drug Resistance1178-69732022-03-01Volume 151383138974083Evaluation of Neisseria gonorrhoeae Isolates Susceptibility to Tetracycline Antibiotics from 9 Provinces in China Since 2020Zhou QXu WZhu XZhang JZhang YHan YChen KYin YQian Zhou,1,2,* Wenqi Xu,1,2,* Xiaoyu Zhu,1,2 Jin Zhang,1,2 Yan Zhang,1,2 Yan Han,1,2 Kai Chen,1,2 Yueping Yin1,2 1Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, People’s Republic of China; 2STD Reference Laboratory, National Center for Sexually Transmitted Diseases Control, Chinese Center for Disease Control and Prevention, Nanjing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yueping Yin, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, People’s Republic of China, Email yinyp@ncstdlc.orgPurpose: The increasing drug resistance of Neisseria gonorrhoeae has become a serious public health concern. This study investigated N. gonorrhoeae isolates susceptibility to tetracycline antibiotics and the correlation between minimum inhibitory concentrations (MICs) of different antibiotics. The presence of resistance determinants in N. gonorrhoeae strains displaying different levels of tigecycline resistance was also compared.Methods: The minimum inhibitory concentrations (MICs) of tetracycline, minocycline, tigecycline, eravacycline, omadacycline on 412 N. gonorrhoeae isolates were measured by the agar dilution method. The MICs of ceftriaxone and azithromycin were also measured to determine the correlations between antibiotics by the value of the correlation coefficient R. The presence of resistance determinants was identified through polymerase chain reaction (PCR) and sequencing.Results: The MIC90 was 64mg/L for tetracycline, 64mg/L for minocycline, 0.5mg/L for tigecycline, 0.5mg/L for eravacycline, 4mg/L for omadacycline, 0.25 mg/L for ceftriaxone, and 1mg/L for azithromycin. The MIC90 and mode of tigecycline and eravacycline were much lower than those of tetracycline and minocycline. A poor correlation between omadacycline, eravacycline and tetracycline susceptibility was observed. Minocycline has a strong correlation with tetracycline. PorB1 typing, TetM-encoding plasmid, and mtrR promoter adenine deletion were significantly correlated with tigecycline MIC > 0.25mg/L.Conclusion: This study suggested that tigecycline and eravacycline had better in vitro activity and might be alternative antibiotics against resistant N. gonorrhoeae infections. Nevertheless, further in vitro experiments and clinical studies are needed for verification.Keywords: Neisseria gonorrhoeae, antibiotics susceptibility, tigecycline, eravacycline, omadacyclinehttps://www.dovepress.com/evaluation-of-neisseria-gonorrhoeae-isolates-susceptibility-to-tetracy-peer-reviewed-fulltext-article-IDRneisseria gonorrhoeaeantibiotics susceptibilitytigecyclineeravacyclineomadacycline |
| spellingShingle | Zhou Q Xu W Zhu X Zhang J Zhang Y Han Y Chen K Yin Y Evaluation of Neisseria gonorrhoeae Isolates Susceptibility to Tetracycline Antibiotics from 9 Provinces in China Since 2020 Infection and Drug Resistance neisseria gonorrhoeae antibiotics susceptibility tigecycline eravacycline omadacycline |
| title | Evaluation of Neisseria gonorrhoeae Isolates Susceptibility to Tetracycline Antibiotics from 9 Provinces in China Since 2020 |
| title_full | Evaluation of Neisseria gonorrhoeae Isolates Susceptibility to Tetracycline Antibiotics from 9 Provinces in China Since 2020 |
| title_fullStr | Evaluation of Neisseria gonorrhoeae Isolates Susceptibility to Tetracycline Antibiotics from 9 Provinces in China Since 2020 |
| title_full_unstemmed | Evaluation of Neisseria gonorrhoeae Isolates Susceptibility to Tetracycline Antibiotics from 9 Provinces in China Since 2020 |
| title_short | Evaluation of Neisseria gonorrhoeae Isolates Susceptibility to Tetracycline Antibiotics from 9 Provinces in China Since 2020 |
| title_sort | evaluation of neisseria gonorrhoeae isolates susceptibility to tetracycline antibiotics from 9 provinces in china since 2020 |
| topic | neisseria gonorrhoeae antibiotics susceptibility tigecycline eravacycline omadacycline |
| url | https://www.dovepress.com/evaluation-of-neisseria-gonorrhoeae-isolates-susceptibility-to-tetracy-peer-reviewed-fulltext-article-IDR |
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