Rare mutations in Pfmdr1 gene of Plasmodium falciparum detected in clinical isolates from patients treated with anti-malarial drug in Nigeria
Abstract Background Plasmodium falciparum, the deadliest causative agent of malaria, has high prevalence in Nigeria. Drug resistance causing failure of previously effective drugs has compromised anti-malarial treatment. On this basis, there is need for a proactive surveillance for resistance markers...
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| Format: | Article |
| Language: | English |
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BMC
2019-09-01
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| Series: | Malaria Journal |
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| Online Access: | http://link.springer.com/article/10.1186/s12936-019-2947-z |
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| author | Abel O. Idowu Wellington A. Oyibo Sanjib Bhattacharyya Manjeet Khubbar Udoma E. Mendie Violet V. Bumah Carolyn Black Joseph Igietseme Anthony A. Azenabor |
| author_facet | Abel O. Idowu Wellington A. Oyibo Sanjib Bhattacharyya Manjeet Khubbar Udoma E. Mendie Violet V. Bumah Carolyn Black Joseph Igietseme Anthony A. Azenabor |
| author_sort | Abel O. Idowu |
| collection | DOAJ |
| description | Abstract Background Plasmodium falciparum, the deadliest causative agent of malaria, has high prevalence in Nigeria. Drug resistance causing failure of previously effective drugs has compromised anti-malarial treatment. On this basis, there is need for a proactive surveillance for resistance markers to the currently recommended artemisinin-based combination therapy (ACT), for early detection of resistance before it become widespread. Methods This study assessed anti-malarial resistance genes polymorphism in patients with uncomplicated P. falciparum malaria in Lagos, Nigeria. Sanger and Next Generation Sequencing (NGS) methods were used to screen for mutations in thirty-seven malaria positive blood samples targeting the P. falciparum chloroquine-resistance transporter (Pfcrt), P. falciparum multidrug-resistance 1 (Pfmdr1), and P. falciparum kelch 13 (Pfk13) genes, which have been previously associated with anti-malarial resistance. Results Expectedly, the NGS method was more proficient, detecting six Pfmdr1, seven Pfcrt and three Pfk13 mutations in the studied clinical isolates from Nigeria, a malaria endemic area. These mutations included rare Pfmdr1 mutations, N504K, N649D, F938Y and S967N, which were previously unreported. In addition, there was moderate prevalence of the K76T mutation (34.6%) associated with chloroquine and amodiaquine resistance, and high prevalence of the N86 wild type allele (92.3%) associated with lumefantrine resistance. Conclusion Widespread circulation of mutations associated with resistance to current anti-malarial drugs could potentially limit effective malaria therapy in endemic populations. |
| first_indexed | 2024-12-23T04:17:34Z |
| format | Article |
| id | doaj.art-3aa355f8e1ea47b5a76305f3216da137 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-23T04:17:34Z |
| publishDate | 2019-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-3aa355f8e1ea47b5a76305f3216da1372022-12-21T18:00:21ZengBMCMalaria Journal1475-28752019-09-011811910.1186/s12936-019-2947-zRare mutations in Pfmdr1 gene of Plasmodium falciparum detected in clinical isolates from patients treated with anti-malarial drug in NigeriaAbel O. Idowu0Wellington A. Oyibo1Sanjib Bhattacharyya2Manjeet Khubbar3Udoma E. Mendie4Violet V. Bumah5Carolyn Black6Joseph Igietseme7Anthony A. Azenabor8Department of Biomedical Sciences, College of Health Sciences, University of WisconsinANDI Centre of Excellence in Malaria Diagnosis, College of Medicine, University of LagosCity of Milwaukee Health Department LaboratoryCity of Milwaukee Health Department LaboratoryDepartment of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of LagosDepartment of Biology, North Life Science 317, San Diego State UniversityMolecular Pathogenesis Laboratory, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and PreventionMolecular Pathogenesis Laboratory, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and PreventionDepartment of Biomedical Sciences, College of Health Sciences, University of WisconsinAbstract Background Plasmodium falciparum, the deadliest causative agent of malaria, has high prevalence in Nigeria. Drug resistance causing failure of previously effective drugs has compromised anti-malarial treatment. On this basis, there is need for a proactive surveillance for resistance markers to the currently recommended artemisinin-based combination therapy (ACT), for early detection of resistance before it become widespread. Methods This study assessed anti-malarial resistance genes polymorphism in patients with uncomplicated P. falciparum malaria in Lagos, Nigeria. Sanger and Next Generation Sequencing (NGS) methods were used to screen for mutations in thirty-seven malaria positive blood samples targeting the P. falciparum chloroquine-resistance transporter (Pfcrt), P. falciparum multidrug-resistance 1 (Pfmdr1), and P. falciparum kelch 13 (Pfk13) genes, which have been previously associated with anti-malarial resistance. Results Expectedly, the NGS method was more proficient, detecting six Pfmdr1, seven Pfcrt and three Pfk13 mutations in the studied clinical isolates from Nigeria, a malaria endemic area. These mutations included rare Pfmdr1 mutations, N504K, N649D, F938Y and S967N, which were previously unreported. In addition, there was moderate prevalence of the K76T mutation (34.6%) associated with chloroquine and amodiaquine resistance, and high prevalence of the N86 wild type allele (92.3%) associated with lumefantrine resistance. Conclusion Widespread circulation of mutations associated with resistance to current anti-malarial drugs could potentially limit effective malaria therapy in endemic populations.http://link.springer.com/article/10.1186/s12936-019-2947-zMalariaInfectious disease epidemiologyAntimalarial gene polymorphismParasitic disease epidemiology and controlPlasmodium |
| spellingShingle | Abel O. Idowu Wellington A. Oyibo Sanjib Bhattacharyya Manjeet Khubbar Udoma E. Mendie Violet V. Bumah Carolyn Black Joseph Igietseme Anthony A. Azenabor Rare mutations in Pfmdr1 gene of Plasmodium falciparum detected in clinical isolates from patients treated with anti-malarial drug in Nigeria Malaria Journal Malaria Infectious disease epidemiology Antimalarial gene polymorphism Parasitic disease epidemiology and control Plasmodium |
| title | Rare mutations in Pfmdr1 gene of Plasmodium falciparum detected in clinical isolates from patients treated with anti-malarial drug in Nigeria |
| title_full | Rare mutations in Pfmdr1 gene of Plasmodium falciparum detected in clinical isolates from patients treated with anti-malarial drug in Nigeria |
| title_fullStr | Rare mutations in Pfmdr1 gene of Plasmodium falciparum detected in clinical isolates from patients treated with anti-malarial drug in Nigeria |
| title_full_unstemmed | Rare mutations in Pfmdr1 gene of Plasmodium falciparum detected in clinical isolates from patients treated with anti-malarial drug in Nigeria |
| title_short | Rare mutations in Pfmdr1 gene of Plasmodium falciparum detected in clinical isolates from patients treated with anti-malarial drug in Nigeria |
| title_sort | rare mutations in pfmdr1 gene of plasmodium falciparum detected in clinical isolates from patients treated with anti malarial drug in nigeria |
| topic | Malaria Infectious disease epidemiology Antimalarial gene polymorphism Parasitic disease epidemiology and control Plasmodium |
| url | http://link.springer.com/article/10.1186/s12936-019-2947-z |
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