Shoot Cultures of <i>Vitis vinifera</i> (Vine Grape) Different Cultivars as a Promising Innovative Cosmetic Raw Material—Phytochemical Profiling, Antioxidant Potential, and Whitening Activity
The primary purpose of this work was the initiation and optimization of shoot cultures of different <i>Vitis vinifera</i> L. cultivars: cv. Chardonnay, cv. Hibernal, cv. Riesling, cv. Johanniter, cv. Solaris, cv. Cabernet Cortis, and cv. Regent. Cultures were maintained on 30-day growth...
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MDPI AG
2023-09-01
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| author | Marta Sharafan Magdalena Anna Malinowska Marta Kubicz Paweł Kubica Marin-Pierre Gémin Cécile Abdallah Manon Ferrier Christophe Hano Nathalie Giglioli-Guivarc’h Elżbieta Sikora Arnaud Lanoue Agnieszka Szopa |
| author_facet | Marta Sharafan Magdalena Anna Malinowska Marta Kubicz Paweł Kubica Marin-Pierre Gémin Cécile Abdallah Manon Ferrier Christophe Hano Nathalie Giglioli-Guivarc’h Elżbieta Sikora Arnaud Lanoue Agnieszka Szopa |
| author_sort | Marta Sharafan |
| collection | DOAJ |
| description | The primary purpose of this work was the initiation and optimization of shoot cultures of different <i>Vitis vinifera</i> L. cultivars: cv. Chardonnay, cv. Hibernal, cv. Riesling, cv. Johanniter, cv. Solaris, cv. Cabernet Cortis, and cv. Regent. Cultures were maintained on 30-day growth cycles using two media, Murashige and Skoog (MS) and Schenk and Hildebrandt (SH), with various concentrations of plant growth regulators. Tested media (‘W1’–‘W4’) contained varying concentrations of 6-benzylaminopurine (BA) in addition to indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA). High performance liquid chromatography coupled with mass spectrometry (UPLC–MS) was used for metabolomic profiling. In all tested extracts, 45 compounds were identified (6 amino acids, 4 phenolic acids, 13 flavan-3-ols, 3 flavonols, and 19 stilbenoids). Principal component analysis (PCA) was performed to assess the influence of the genotype and medium on metabolic content. PCA showed that metabolic content was mainly influenced by genotype and to a lesser extent by medium composition. MS media variants induced the amino acid, procyanidin, and flavan-3-ol production. In addition, the antioxidant potential and anti-tyrosinase activity was measured spectrophotometrically. The studies on antioxidant activity clearly reveal very high efficiency in reducing free radicals in the tested extracts. The strongest tyrosinase inhibition capacity was proved for shoots cv. Hibernal cultured in SH medium and supplemented with NAA, with an inhibition of 17.50%. These studies show that in vitro cultures of <i>V. vinifera</i> cvs. can be proposed as an alternative source of plant material that can be potentially used in cosmetic industry. |
| first_indexed | 2024-03-10T21:39:30Z |
| format | Article |
| id | doaj.art-3aa7616fdaf645a798a9251177413429 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T21:39:30Z |
| publishDate | 2023-09-01 |
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| series | Molecules |
| spelling | doaj.art-3aa7616fdaf645a798a92511774134292023-11-19T14:46:40ZengMDPI AGMolecules1420-30492023-09-012819686810.3390/molecules28196868Shoot Cultures of <i>Vitis vinifera</i> (Vine Grape) Different Cultivars as a Promising Innovative Cosmetic Raw Material—Phytochemical Profiling, Antioxidant Potential, and Whitening ActivityMarta Sharafan0Magdalena Anna Malinowska1Marta Kubicz2Paweł Kubica3Marin-Pierre Gémin4Cécile Abdallah5Manon Ferrier6Christophe Hano7Nathalie Giglioli-Guivarc’h8Elżbieta Sikora9Arnaud Lanoue10Agnieszka Szopa11Department of Pharmaceutical Botany, Medical College, Jagiellonian University, Medyczna 9 St., 30-688 Cracow, PolandInstitute of Organic Chemistry and Technology, Faculty of Chemical Engineering and Technology, Cracow University of Technology, 24 Warszawska St., 31-155 Cracow, PolandDepartment of Pharmaceutical Botany, Medical College, Jagiellonian University, Medyczna 9 St., 30-688 Cracow, PolandDepartment of Pharmaceutical Botany, Medical College, Jagiellonian University, Medyczna 9 St., 30-688 Cracow, PolandEA 2106 Biomolecules et Biotechnologies Végétales, UFR des Sciences Pharmaceutiques, Université de Tours, 31 av. Monge, F37200 Tours, FranceEA 2106 Biomolecules et Biotechnologies Végétales, UFR des Sciences Pharmaceutiques, Université de Tours, 31 av. Monge, F37200 Tours, FranceEA 2106 Biomolecules et Biotechnologies Végétales, UFR des Sciences Pharmaceutiques, Université de Tours, 31 av. Monge, F37200 Tours, FranceInstitut de Chimie Organique et Analytique, Universite d’Orleans-CNRS, UMR 7311 BP 6759, CEDEX 2, 45067 Orléans, FranceEA 2106 Biomolecules et Biotechnologies Végétales, UFR des Sciences Pharmaceutiques, Université de Tours, 31 av. Monge, F37200 Tours, FranceInstitute of Organic Chemistry and Technology, Faculty of Chemical Engineering and Technology, Cracow University of Technology, 24 Warszawska St., 31-155 Cracow, PolandEA 2106 Biomolecules et Biotechnologies Végétales, UFR des Sciences Pharmaceutiques, Université de Tours, 31 av. Monge, F37200 Tours, FranceDepartment of Pharmaceutical Botany, Medical College, Jagiellonian University, Medyczna 9 St., 30-688 Cracow, PolandThe primary purpose of this work was the initiation and optimization of shoot cultures of different <i>Vitis vinifera</i> L. cultivars: cv. Chardonnay, cv. Hibernal, cv. Riesling, cv. Johanniter, cv. Solaris, cv. Cabernet Cortis, and cv. Regent. Cultures were maintained on 30-day growth cycles using two media, Murashige and Skoog (MS) and Schenk and Hildebrandt (SH), with various concentrations of plant growth regulators. Tested media (‘W1’–‘W4’) contained varying concentrations of 6-benzylaminopurine (BA) in addition to indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA). High performance liquid chromatography coupled with mass spectrometry (UPLC–MS) was used for metabolomic profiling. In all tested extracts, 45 compounds were identified (6 amino acids, 4 phenolic acids, 13 flavan-3-ols, 3 flavonols, and 19 stilbenoids). Principal component analysis (PCA) was performed to assess the influence of the genotype and medium on metabolic content. PCA showed that metabolic content was mainly influenced by genotype and to a lesser extent by medium composition. MS media variants induced the amino acid, procyanidin, and flavan-3-ol production. In addition, the antioxidant potential and anti-tyrosinase activity was measured spectrophotometrically. The studies on antioxidant activity clearly reveal very high efficiency in reducing free radicals in the tested extracts. The strongest tyrosinase inhibition capacity was proved for shoots cv. Hibernal cultured in SH medium and supplemented with NAA, with an inhibition of 17.50%. These studies show that in vitro cultures of <i>V. vinifera</i> cvs. can be proposed as an alternative source of plant material that can be potentially used in cosmetic industry.https://www.mdpi.com/1420-3049/28/19/6868<i>Vitis vinifera</i>vine grapein vitro culturetissue culturemetabolomic profilingstilbenoids |
| spellingShingle | Marta Sharafan Magdalena Anna Malinowska Marta Kubicz Paweł Kubica Marin-Pierre Gémin Cécile Abdallah Manon Ferrier Christophe Hano Nathalie Giglioli-Guivarc’h Elżbieta Sikora Arnaud Lanoue Agnieszka Szopa Shoot Cultures of <i>Vitis vinifera</i> (Vine Grape) Different Cultivars as a Promising Innovative Cosmetic Raw Material—Phytochemical Profiling, Antioxidant Potential, and Whitening Activity Molecules <i>Vitis vinifera</i> vine grape in vitro culture tissue culture metabolomic profiling stilbenoids |
| title | Shoot Cultures of <i>Vitis vinifera</i> (Vine Grape) Different Cultivars as a Promising Innovative Cosmetic Raw Material—Phytochemical Profiling, Antioxidant Potential, and Whitening Activity |
| title_full | Shoot Cultures of <i>Vitis vinifera</i> (Vine Grape) Different Cultivars as a Promising Innovative Cosmetic Raw Material—Phytochemical Profiling, Antioxidant Potential, and Whitening Activity |
| title_fullStr | Shoot Cultures of <i>Vitis vinifera</i> (Vine Grape) Different Cultivars as a Promising Innovative Cosmetic Raw Material—Phytochemical Profiling, Antioxidant Potential, and Whitening Activity |
| title_full_unstemmed | Shoot Cultures of <i>Vitis vinifera</i> (Vine Grape) Different Cultivars as a Promising Innovative Cosmetic Raw Material—Phytochemical Profiling, Antioxidant Potential, and Whitening Activity |
| title_short | Shoot Cultures of <i>Vitis vinifera</i> (Vine Grape) Different Cultivars as a Promising Innovative Cosmetic Raw Material—Phytochemical Profiling, Antioxidant Potential, and Whitening Activity |
| title_sort | shoot cultures of i vitis vinifera i vine grape different cultivars as a promising innovative cosmetic raw material phytochemical profiling antioxidant potential and whitening activity |
| topic | <i>Vitis vinifera</i> vine grape in vitro culture tissue culture metabolomic profiling stilbenoids |
| url | https://www.mdpi.com/1420-3049/28/19/6868 |
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