Drug susceptibility testing using molecular techniques can enhance diagnosis in a community with a high tuberculosis incidence
Background: Sputum samples were collected from tuberculosis patients in a high tuberculosis incidence area in the Western Cape, South Africa. The aim of this study was to evaluate the performance and time to diagnosis of a genotypic drug susceptibility testing method.Methodology: During June 2000 an...
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| Format: | Article |
| Language: | English |
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The Journal of Infection in Developing Countries
2008-02-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | http://www.oloep.org/jidc/content.asp?id=1159 |
| _version_ | 1818623643508277248 |
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| author | Rabia Johnson,1 Annemie M. Jordaan,1 Rob Warren,1 Marleine Bosman,2 Douglas Young,3 Judit N. Nagy,3 John R. Wain,4 Paul D. van Helden,1 Thomas C. Victor.1 |
| author_facet | Rabia Johnson,1 Annemie M. Jordaan,1 Rob Warren,1 Marleine Bosman,2 Douglas Young,3 Judit N. Nagy,3 John R. Wain,4 Paul D. van Helden,1 Thomas C. Victor.1 |
| author_sort | Rabia Johnson,1 Annemie M. Jordaan,1 Rob Warren,1 Marleine Bosman,2 Douglas Young,3 Judit N. Nagy,3 John R. Wain,4 Paul D. van Helden,1 Thomas C. Victor.1 |
| collection | DOAJ |
| description | Background: Sputum samples were collected from tuberculosis patients in a high tuberculosis incidence area in the Western Cape, South Africa. The aim of this study was to evaluate the performance and time to diagnosis of a genotypic drug susceptibility testing method.Methodology: During June 2000 and November 2003, a total of 1,540 samples were sent for drug susceptibility testing (DST) to the national health laboratory services, and of those, a phenotypic DST result was obtained for 1,373 samples whereas a genotypic DST result was obtained for 1,301 of 1,540 samples. Performance-based calculations were done on 1,244 samples for which both a phenotypic and genotypic DST result was available.Results: The reproducibility of the genotypic and phenotypic DST methods was 97% and 95%, respectively. The sensitivity and specificity of the genotypic DST method was 68% and 99% for Isoniazid and 87% and 99% for Rifampicin, respectively. Smear gradation was found to influence the performance of the genotypic DST method. The genotypic DST method gave accurate DST results for 75% of the samples within 20 days (range, 15-25), whereas the phenotypic DST results were only available for 75% of the samples after 38 days (range, 26-115) (p<0.001).Conclusion: This study showed that the genotypic DST could improve tuberculosis control by rapid diagnosis of drug resistant tuberculosis. This finding may have important implications for the control of drug resistant tuberculosis as it may reduce the chance for further transmission events. |
| first_indexed | 2024-12-16T18:44:20Z |
| format | Article |
| id | doaj.art-3aa76c7edef047b8a8cf5598816b48f5 |
| institution | Directory Open Access Journal |
| issn | 1972-2680 |
| language | English |
| last_indexed | 2024-12-16T18:44:20Z |
| publishDate | 2008-02-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj.art-3aa76c7edef047b8a8cf5598816b48f52022-12-21T22:20:54ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802008-02-01214045Drug susceptibility testing using molecular techniques can enhance diagnosis in a community with a high tuberculosis incidenceRabia Johnson,1 Annemie M. Jordaan,1 Rob Warren,1 Marleine Bosman,2 Douglas Young,3 Judit N. Nagy,3 John R. Wain,4 Paul D. van Helden,1 Thomas C. Victor.1Background: Sputum samples were collected from tuberculosis patients in a high tuberculosis incidence area in the Western Cape, South Africa. The aim of this study was to evaluate the performance and time to diagnosis of a genotypic drug susceptibility testing method.Methodology: During June 2000 and November 2003, a total of 1,540 samples were sent for drug susceptibility testing (DST) to the national health laboratory services, and of those, a phenotypic DST result was obtained for 1,373 samples whereas a genotypic DST result was obtained for 1,301 of 1,540 samples. Performance-based calculations were done on 1,244 samples for which both a phenotypic and genotypic DST result was available.Results: The reproducibility of the genotypic and phenotypic DST methods was 97% and 95%, respectively. The sensitivity and specificity of the genotypic DST method was 68% and 99% for Isoniazid and 87% and 99% for Rifampicin, respectively. Smear gradation was found to influence the performance of the genotypic DST method. The genotypic DST method gave accurate DST results for 75% of the samples within 20 days (range, 15-25), whereas the phenotypic DST results were only available for 75% of the samples after 38 days (range, 26-115) (p<0.001).Conclusion: This study showed that the genotypic DST could improve tuberculosis control by rapid diagnosis of drug resistant tuberculosis. This finding may have important implications for the control of drug resistant tuberculosis as it may reduce the chance for further transmission events.http://www.oloep.org/jidc/content.asp?id=1159drug resistant phenotypedrug resistant genotypeprobe methodtime to positivitysmear gradationrapid diagnosis |
| spellingShingle | Rabia Johnson,1 Annemie M. Jordaan,1 Rob Warren,1 Marleine Bosman,2 Douglas Young,3 Judit N. Nagy,3 John R. Wain,4 Paul D. van Helden,1 Thomas C. Victor.1 Drug susceptibility testing using molecular techniques can enhance diagnosis in a community with a high tuberculosis incidence Journal of Infection in Developing Countries drug resistant phenotype drug resistant genotype probe method time to positivity smear gradation rapid diagnosis |
| title | Drug susceptibility testing using molecular techniques can enhance diagnosis in a community with a high tuberculosis incidence |
| title_full | Drug susceptibility testing using molecular techniques can enhance diagnosis in a community with a high tuberculosis incidence |
| title_fullStr | Drug susceptibility testing using molecular techniques can enhance diagnosis in a community with a high tuberculosis incidence |
| title_full_unstemmed | Drug susceptibility testing using molecular techniques can enhance diagnosis in a community with a high tuberculosis incidence |
| title_short | Drug susceptibility testing using molecular techniques can enhance diagnosis in a community with a high tuberculosis incidence |
| title_sort | drug susceptibility testing using molecular techniques can enhance diagnosis in a community with a high tuberculosis incidence |
| topic | drug resistant phenotype drug resistant genotype probe method time to positivity smear gradation rapid diagnosis |
| url | http://www.oloep.org/jidc/content.asp?id=1159 |
| work_keys_str_mv | AT rabiajohnson1annemiemjordaan1robwarren1marleinebosman2douglasyoung3juditnnagy3johnrwain4pauldvanhelden1thomascvictor1 drugsusceptibilitytestingusingmoleculartechniquescanenhancediagnosisinacommunitywithahightuberculosisincidence |