The influence of etomidate on connexons of induced differentiated neurons in mouse
Objective To explore the effect of general anesthetic, etomidate, on the expression of connexons in neurons induced by P19 cells, and illustrate the molecular mechanism of patients' consciousness loss after etomidate administration by means of immunocytochemistry. Methods The P19 cells were ret...
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| Format: | Article |
| Language: | English |
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Tianjin Huanhu Hospital
2010-08-01
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| Series: | Chinese Journal of Contemporary Neurology and Neurosurgery |
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| Online Access: | http://www.cjcnn.org/index.php/cjcnn/article/view/485 |
| _version_ | 1819102797159727104 |
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| author | Zheng QI Xu JIN Bao⁃guo WANG |
| author_facet | Zheng QI Xu JIN Bao⁃guo WANG |
| author_sort | Zheng QI |
| collection | DOAJ |
| description | Objective To explore the effect of general anesthetic, etomidate, on the expression of connexons in neurons induced by P19 cells, and illustrate the molecular mechanism of patients' consciousness loss after etomidate administration by means of immunocytochemistry. Methods The P19 cells were retro ⁃ induced to stem cells, which were then oriented ⁃ induced to neurons to receive neuron identification. The neurons were then allocated into group E (etomidate), group CAR (carbenoxolone), group LE (lipid emulsion) and group CON (phosphate buffer solution, control). Agents administrated respectively were withdrawn in 30 s, 60 min and 120 min, and connexin36 (Cx36) were counted through inference of immunocytochemistry. Results Cx36 were more suppressed for group E and group CAR than group CON in 30 s and 60 min (30 s: t = ⁃ 50.423, P = 0.000; 60 min: t = ⁃ 25.826, P = 0.000). As for the inner group analysis, group E and group CAR bared declined suppressive effects with even no statistic variance in 120 min compared to the respective untreated time points; as for group LE and group CON, no statistic variance existed between different times. Intergroup analysis showed that significant suppressive effects in 30 s were seen in group E and group CAR which were more than group LE and group CON (P < 0.05, for all) while the difference of suppressive effects were not seen between group E and group CAR (P > 0.05). In 60 min, positive ⁃ stained connexons were more suppressed in group E than in group CAR (t = ⁃ 2.782, P = 0.008). Etomidate gave more suppression than carbenoxolone and this stronger effect was not shown until 60 min. Conclusion Etomidate depresses the gap junction intercellular connection (GJIC) by means of decreasing the number of neuron connexons, which might be the molecular mechanism for patients' consciousness loss after etomidate administration.
DOI：10.3969/j.issn.1672-6731.2010.04.015 |
| first_indexed | 2024-12-22T01:40:16Z |
| format | Article |
| id | doaj.art-3aa850a5b9c24110a112a27acc235752 |
| institution | Directory Open Access Journal |
| issn | 1672-6731 |
| language | English |
| last_indexed | 2024-12-22T01:40:16Z |
| publishDate | 2010-08-01 |
| publisher | Tianjin Huanhu Hospital |
| record_format | Article |
| series | Chinese Journal of Contemporary Neurology and Neurosurgery |
| spelling | doaj.art-3aa850a5b9c24110a112a27acc2357522022-12-21T18:43:16ZengTianjin Huanhu HospitalChinese Journal of Contemporary Neurology and Neurosurgery1672-67312010-08-01104462467484The influence of etomidate on connexons of induced differentiated neurons in mouseZheng QIXu JINBao⁃guo WANGObjective To explore the effect of general anesthetic, etomidate, on the expression of connexons in neurons induced by P19 cells, and illustrate the molecular mechanism of patients' consciousness loss after etomidate administration by means of immunocytochemistry. Methods The P19 cells were retro ⁃ induced to stem cells, which were then oriented ⁃ induced to neurons to receive neuron identification. The neurons were then allocated into group E (etomidate), group CAR (carbenoxolone), group LE (lipid emulsion) and group CON (phosphate buffer solution, control). Agents administrated respectively were withdrawn in 30 s, 60 min and 120 min, and connexin36 (Cx36) were counted through inference of immunocytochemistry. Results Cx36 were more suppressed for group E and group CAR than group CON in 30 s and 60 min (30 s: t = ⁃ 50.423, P = 0.000; 60 min: t = ⁃ 25.826, P = 0.000). As for the inner group analysis, group E and group CAR bared declined suppressive effects with even no statistic variance in 120 min compared to the respective untreated time points; as for group LE and group CON, no statistic variance existed between different times. Intergroup analysis showed that significant suppressive effects in 30 s were seen in group E and group CAR which were more than group LE and group CON (P < 0.05, for all) while the difference of suppressive effects were not seen between group E and group CAR (P > 0.05). In 60 min, positive ⁃ stained connexons were more suppressed in group E than in group CAR (t = ⁃ 2.782, P = 0.008). Etomidate gave more suppression than carbenoxolone and this stronger effect was not shown until 60 min. Conclusion Etomidate depresses the gap junction intercellular connection (GJIC) by means of decreasing the number of neuron connexons, which might be the molecular mechanism for patients' consciousness loss after etomidate administration. DOI：10.3969/j.issn.1672-6731.2010.04.015http://www.cjcnn.org/index.php/cjcnn/article/view/485EtomidateAnesthesia, generalConnexinsGap junctionsCells, cultured |
| spellingShingle | Zheng QI Xu JIN Bao⁃guo WANG The influence of etomidate on connexons of induced differentiated neurons in mouse Chinese Journal of Contemporary Neurology and Neurosurgery Etomidate Anesthesia, general Connexins Gap junctions Cells, cultured |
| title | The influence of etomidate on connexons of induced differentiated neurons in mouse |
| title_full | The influence of etomidate on connexons of induced differentiated neurons in mouse |
| title_fullStr | The influence of etomidate on connexons of induced differentiated neurons in mouse |
| title_full_unstemmed | The influence of etomidate on connexons of induced differentiated neurons in mouse |
| title_short | The influence of etomidate on connexons of induced differentiated neurons in mouse |
| title_sort | influence of etomidate on connexons of induced differentiated neurons in mouse |
| topic | Etomidate Anesthesia, general Connexins Gap junctions Cells, cultured |
| url | http://www.cjcnn.org/index.php/cjcnn/article/view/485 |
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