Regulation of neuronal morphogenesis and positioning by ubiquitin-specific proteases in the cerebellum.
Ubiquitin signaling mechanisms play fundamental roles in the cell-intrinsic control of neuronal morphogenesis and connectivity in the brain. However, whereas specific ubiquitin ligases have been implicated in key steps of neural circuit assembly, the roles of ubiquitin-specific proteases (USPs) in t...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4301861?pdf=render |
| _version_ | 1819048165821644800 |
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| author | Julius Anckar Azad Bonni |
| author_facet | Julius Anckar Azad Bonni |
| author_sort | Julius Anckar |
| collection | DOAJ |
| description | Ubiquitin signaling mechanisms play fundamental roles in the cell-intrinsic control of neuronal morphogenesis and connectivity in the brain. However, whereas specific ubiquitin ligases have been implicated in key steps of neural circuit assembly, the roles of ubiquitin-specific proteases (USPs) in the establishment of neuronal connectivity have remained unexplored. Here, we report a comprehensive analysis of USP family members in granule neuron morphogenesis and positioning in the rodent cerebellum. We identify a set of 32 USPs that are expressed in granule neurons. We also characterize the subcellular localization of the 32 USPs in granule neurons using a library of expression plasmids encoding GFP-USPs. In RNAi screens of the 32 neuronally expressed USPs, we uncover novel functions for USP1, USP4, and USP20 in the morphogenesis of granule neuron dendrites and axons and we identify a requirement for USP30 and USP33 in granule neuron migration in the rodent cerebellar cortex in vivo. These studies reveal that specific USPs with distinct spatial localizations harbor key functions in the control of neuronal morphogenesis and positioning in the mammalian cerebellum, with important implications for our understanding of the cell-intrinsic mechanisms that govern neural circuit assembly in the brain. |
| first_indexed | 2024-12-21T11:11:56Z |
| format | Article |
| id | doaj.art-3aaea07342304384b543dbe1a4b70c5c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T11:11:56Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3aaea07342304384b543dbe1a4b70c5c2022-12-21T19:06:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01101e011707610.1371/journal.pone.0117076Regulation of neuronal morphogenesis and positioning by ubiquitin-specific proteases in the cerebellum.Julius AnckarAzad BonniUbiquitin signaling mechanisms play fundamental roles in the cell-intrinsic control of neuronal morphogenesis and connectivity in the brain. However, whereas specific ubiquitin ligases have been implicated in key steps of neural circuit assembly, the roles of ubiquitin-specific proteases (USPs) in the establishment of neuronal connectivity have remained unexplored. Here, we report a comprehensive analysis of USP family members in granule neuron morphogenesis and positioning in the rodent cerebellum. We identify a set of 32 USPs that are expressed in granule neurons. We also characterize the subcellular localization of the 32 USPs in granule neurons using a library of expression plasmids encoding GFP-USPs. In RNAi screens of the 32 neuronally expressed USPs, we uncover novel functions for USP1, USP4, and USP20 in the morphogenesis of granule neuron dendrites and axons and we identify a requirement for USP30 and USP33 in granule neuron migration in the rodent cerebellar cortex in vivo. These studies reveal that specific USPs with distinct spatial localizations harbor key functions in the control of neuronal morphogenesis and positioning in the mammalian cerebellum, with important implications for our understanding of the cell-intrinsic mechanisms that govern neural circuit assembly in the brain.http://europepmc.org/articles/PMC4301861?pdf=render |
| spellingShingle | Julius Anckar Azad Bonni Regulation of neuronal morphogenesis and positioning by ubiquitin-specific proteases in the cerebellum. PLoS ONE |
| title | Regulation of neuronal morphogenesis and positioning by ubiquitin-specific proteases in the cerebellum. |
| title_full | Regulation of neuronal morphogenesis and positioning by ubiquitin-specific proteases in the cerebellum. |
| title_fullStr | Regulation of neuronal morphogenesis and positioning by ubiquitin-specific proteases in the cerebellum. |
| title_full_unstemmed | Regulation of neuronal morphogenesis and positioning by ubiquitin-specific proteases in the cerebellum. |
| title_short | Regulation of neuronal morphogenesis and positioning by ubiquitin-specific proteases in the cerebellum. |
| title_sort | regulation of neuronal morphogenesis and positioning by ubiquitin specific proteases in the cerebellum |
| url | http://europepmc.org/articles/PMC4301861?pdf=render |
| work_keys_str_mv | AT juliusanckar regulationofneuronalmorphogenesisandpositioningbyubiquitinspecificproteasesinthecerebellum AT azadbonni regulationofneuronalmorphogenesisandpositioningbyubiquitinspecificproteasesinthecerebellum |