The formation and accumulation of protein-networks by physical interactions in the rapid occlusion of laticifer cells in rubber tree undergoing successive mechanical wounding
Abstract Background Although the wound response of plants has been extensively studied, little is known of the rapid occlusion of wounded cell itself. The laticifer in rubber tree is a specific type of tissue for natural rubber biosynthesis and storage. In natural rubber production, tapping is used...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-018-1617-6 |
| _version_ | 1811337129198878720 |
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| author | Minjing Shi Yan Li Shunnan Deng Dongdong Wang Yueyi Chen Shuguang Yang Jilin Wu Wei-Min Tian |
| author_facet | Minjing Shi Yan Li Shunnan Deng Dongdong Wang Yueyi Chen Shuguang Yang Jilin Wu Wei-Min Tian |
| author_sort | Minjing Shi |
| collection | DOAJ |
| description | Abstract Background Although the wound response of plants has been extensively studied, little is known of the rapid occlusion of wounded cell itself. The laticifer in rubber tree is a specific type of tissue for natural rubber biosynthesis and storage. In natural rubber production, tapping is used to harvest the latex which flows out from the severed laticifer in the bark. Therefore, study of the rapid wound-occlusion of severed laticifer cells is important for understanding the rubber tree being protected from the continuously mechanical wounding. Results Using cytological and biochemical techniques, we revealed a biochemical mechanism for the rapid occlusion of severed laticifer cells. A protein-network appeared rapidly after tapping and accumulated gradually along with the latex loss at the severed site of laticifer cells. Triple immunofluorescence histochemical localization showed that the primary components of the protein-network were chitinase, β-1,3-glucanase and hevein together with pro-hevein (ProH) and its carboxyl-terminal part. Molecular sieve chromatography showed that the physical interactions among these proteins occurred under the condition of neutral pH. The interaction of β-1,3-glucanase respectively with hevein, chitinase and ProH was testified by surface plasmon resonance (SPR). The interaction between actin and β-1,3-glucanase out of the protein inclusions of lutoids was revealed by pull-down. This interaction was pharmacologically verified by cytochalasin B–caused significant prolongation of the duration of latex flow in the field. Conclusions The formation of protein-network by interactions of the proteins with anti-pathogen activity released from lutoids and accumulation of protein-network by binding to the cytoskeleton are crucial for the rapid occlusion of laticifer cells in rubber tree. The protein-network at the wounded site of laticifer cells provides not only a physical barrier but also a biochemical barrier to protect the wounded laticifer cells from pathogen invasion. |
| first_indexed | 2024-04-13T17:50:24Z |
| format | Article |
| id | doaj.art-3aaee40d591847f5ba9e14ebd5f17c25 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-13T17:50:24Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-3aaee40d591847f5ba9e14ebd5f17c252022-12-22T02:36:44ZengBMCBMC Plant Biology1471-22292019-01-0119111310.1186/s12870-018-1617-6The formation and accumulation of protein-networks by physical interactions in the rapid occlusion of laticifer cells in rubber tree undergoing successive mechanical woundingMinjing Shi0Yan Li1Shunnan Deng2Dongdong Wang3Yueyi Chen4Shuguang Yang5Jilin Wu6Wei-Min Tian7Institute of Rubber Research, Chinese Academy of Tropical Agricultural SciencesInstitute of Rubber Research, Chinese Academy of Tropical Agricultural SciencesInstitute of Rubber Research, Chinese Academy of Tropical Agricultural SciencesInstitute of Rubber Research, Chinese Academy of Tropical Agricultural SciencesInstitute of Rubber Research, Chinese Academy of Tropical Agricultural SciencesInstitute of Rubber Research, Chinese Academy of Tropical Agricultural SciencesInstitute of Rubber Research, Chinese Academy of Tropical Agricultural SciencesInstitute of Rubber Research, Chinese Academy of Tropical Agricultural SciencesAbstract Background Although the wound response of plants has been extensively studied, little is known of the rapid occlusion of wounded cell itself. The laticifer in rubber tree is a specific type of tissue for natural rubber biosynthesis and storage. In natural rubber production, tapping is used to harvest the latex which flows out from the severed laticifer in the bark. Therefore, study of the rapid wound-occlusion of severed laticifer cells is important for understanding the rubber tree being protected from the continuously mechanical wounding. Results Using cytological and biochemical techniques, we revealed a biochemical mechanism for the rapid occlusion of severed laticifer cells. A protein-network appeared rapidly after tapping and accumulated gradually along with the latex loss at the severed site of laticifer cells. Triple immunofluorescence histochemical localization showed that the primary components of the protein-network were chitinase, β-1,3-glucanase and hevein together with pro-hevein (ProH) and its carboxyl-terminal part. Molecular sieve chromatography showed that the physical interactions among these proteins occurred under the condition of neutral pH. The interaction of β-1,3-glucanase respectively with hevein, chitinase and ProH was testified by surface plasmon resonance (SPR). The interaction between actin and β-1,3-glucanase out of the protein inclusions of lutoids was revealed by pull-down. This interaction was pharmacologically verified by cytochalasin B–caused significant prolongation of the duration of latex flow in the field. Conclusions The formation of protein-network by interactions of the proteins with anti-pathogen activity released from lutoids and accumulation of protein-network by binding to the cytoskeleton are crucial for the rapid occlusion of laticifer cells in rubber tree. The protein-network at the wounded site of laticifer cells provides not only a physical barrier but also a biochemical barrier to protect the wounded laticifer cells from pathogen invasion.http://link.springer.com/article/10.1186/s12870-018-1617-6Hevea brasilensis Muell. Arg.Laticifer cellProtein-networkProtein interactionCytoskeletonMechanical wounding |
| spellingShingle | Minjing Shi Yan Li Shunnan Deng Dongdong Wang Yueyi Chen Shuguang Yang Jilin Wu Wei-Min Tian The formation and accumulation of protein-networks by physical interactions in the rapid occlusion of laticifer cells in rubber tree undergoing successive mechanical wounding BMC Plant Biology Hevea brasilensis Muell. Arg. Laticifer cell Protein-network Protein interaction Cytoskeleton Mechanical wounding |
| title | The formation and accumulation of protein-networks by physical interactions in the rapid occlusion of laticifer cells in rubber tree undergoing successive mechanical wounding |
| title_full | The formation and accumulation of protein-networks by physical interactions in the rapid occlusion of laticifer cells in rubber tree undergoing successive mechanical wounding |
| title_fullStr | The formation and accumulation of protein-networks by physical interactions in the rapid occlusion of laticifer cells in rubber tree undergoing successive mechanical wounding |
| title_full_unstemmed | The formation and accumulation of protein-networks by physical interactions in the rapid occlusion of laticifer cells in rubber tree undergoing successive mechanical wounding |
| title_short | The formation and accumulation of protein-networks by physical interactions in the rapid occlusion of laticifer cells in rubber tree undergoing successive mechanical wounding |
| title_sort | formation and accumulation of protein networks by physical interactions in the rapid occlusion of laticifer cells in rubber tree undergoing successive mechanical wounding |
| topic | Hevea brasilensis Muell. Arg. Laticifer cell Protein-network Protein interaction Cytoskeleton Mechanical wounding |
| url | http://link.springer.com/article/10.1186/s12870-018-1617-6 |
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