Essential Amino Acid Starvation-Induced Oxidative Stress Causes DNA Damage and Apoptosis in Murine Osteoblast-like Cells
Intracellular nutrient metabolism, particularly the metabolism of essential amino acids (EAAs), is crucial for cellular functions, including energy production and redox homeostasis. An EAA deficiency can lead to cellular dysfunction and oxidative stress. This study explores the mechanisms underlying...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/20/15314 |
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| author | Runbo Li Hirohito Kato Chihiro Fumimoto Yurika Nakamura Kimihiro Yoshimura Emika Minagawa Keiju Omatsu Chizuko Ogata Yoichiro Taguchi Makoto Umeda |
| author_facet | Runbo Li Hirohito Kato Chihiro Fumimoto Yurika Nakamura Kimihiro Yoshimura Emika Minagawa Keiju Omatsu Chizuko Ogata Yoichiro Taguchi Makoto Umeda |
| author_sort | Runbo Li |
| collection | DOAJ |
| description | Intracellular nutrient metabolism, particularly the metabolism of essential amino acids (EAAs), is crucial for cellular functions, including energy production and redox homeostasis. An EAA deficiency can lead to cellular dysfunction and oxidative stress. This study explores the mechanisms underlying cellular responses to EAA starvation, focusing on ROS-induced DNA damage and apoptosis. MC3T3-E1 cells were subjected to EAA starvation, and various assays were conducted to assess cell proliferation, survival, DNA damage, and apoptosis. The antioxidant N-acetylcysteine (NAC) was employed to block ROS formation and mitigate cellular damage. Gene expression and Western blot analyses were performed to elucidate molecular pathways. EAA starvation-induced ROS generation, DNA damage, and apoptosis in MC3T3-E1 cells. NAC administration effectively reduced DNA damage and apoptosis, highlighting the pivotal role of ROS in mediating these cellular responses during EAA deficiency. This study demonstrates that EAA starvation triggers ROS-mediated DNA damage and apoptosis, offering insights into the intricate interplay between nutrient deficiency, oxidative stress, and programmed cell death. NAC emerges as a potential therapeutic intervention to counteract these adverse effects. |
| first_indexed | 2024-03-10T21:11:36Z |
| format | Article |
| id | doaj.art-3aaf594d10a84e06889ba44e9b4b714e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T21:11:36Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3aaf594d10a84e06889ba44e9b4b714e2023-11-19T16:44:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124201531410.3390/ijms242015314Essential Amino Acid Starvation-Induced Oxidative Stress Causes DNA Damage and Apoptosis in Murine Osteoblast-like CellsRunbo Li0Hirohito Kato1Chihiro Fumimoto2Yurika Nakamura3Kimihiro Yoshimura4Emika Minagawa5Keiju Omatsu6Chizuko Ogata7Yoichiro Taguchi8Makoto Umeda9Department of Periodontology, Osaka Dental University, Hirakata, Osaka 573-1121, JapanDepartment of Periodontology, Osaka Dental University, Hirakata, Osaka 573-1121, JapanDepartment of Periodontology, Osaka Dental University, Hirakata, Osaka 573-1121, JapanDepartment of Periodontology, Osaka Dental University, Hirakata, Osaka 573-1121, JapanDepartment of Periodontology, Osaka Dental University, Hirakata, Osaka 573-1121, JapanDepartment of Periodontology, Osaka Dental University, Hirakata, Osaka 573-1121, JapanDepartment of Periodontology, Osaka Dental University, Hirakata, Osaka 573-1121, JapanDepartment of Periodontology, Osaka Dental University, Hirakata, Osaka 573-1121, JapanDepartment of Periodontology, Osaka Dental University, Hirakata, Osaka 573-1121, JapanDepartment of Periodontology, Osaka Dental University, Hirakata, Osaka 573-1121, JapanIntracellular nutrient metabolism, particularly the metabolism of essential amino acids (EAAs), is crucial for cellular functions, including energy production and redox homeostasis. An EAA deficiency can lead to cellular dysfunction and oxidative stress. This study explores the mechanisms underlying cellular responses to EAA starvation, focusing on ROS-induced DNA damage and apoptosis. MC3T3-E1 cells were subjected to EAA starvation, and various assays were conducted to assess cell proliferation, survival, DNA damage, and apoptosis. The antioxidant N-acetylcysteine (NAC) was employed to block ROS formation and mitigate cellular damage. Gene expression and Western blot analyses were performed to elucidate molecular pathways. EAA starvation-induced ROS generation, DNA damage, and apoptosis in MC3T3-E1 cells. NAC administration effectively reduced DNA damage and apoptosis, highlighting the pivotal role of ROS in mediating these cellular responses during EAA deficiency. This study demonstrates that EAA starvation triggers ROS-mediated DNA damage and apoptosis, offering insights into the intricate interplay between nutrient deficiency, oxidative stress, and programmed cell death. NAC emerges as a potential therapeutic intervention to counteract these adverse effects.https://www.mdpi.com/1422-0067/24/20/15314essential amino acidsapoptosisROSosteoblasts |
| spellingShingle | Runbo Li Hirohito Kato Chihiro Fumimoto Yurika Nakamura Kimihiro Yoshimura Emika Minagawa Keiju Omatsu Chizuko Ogata Yoichiro Taguchi Makoto Umeda Essential Amino Acid Starvation-Induced Oxidative Stress Causes DNA Damage and Apoptosis in Murine Osteoblast-like Cells International Journal of Molecular Sciences essential amino acids apoptosis ROS osteoblasts |
| title | Essential Amino Acid Starvation-Induced Oxidative Stress Causes DNA Damage and Apoptosis in Murine Osteoblast-like Cells |
| title_full | Essential Amino Acid Starvation-Induced Oxidative Stress Causes DNA Damage and Apoptosis in Murine Osteoblast-like Cells |
| title_fullStr | Essential Amino Acid Starvation-Induced Oxidative Stress Causes DNA Damage and Apoptosis in Murine Osteoblast-like Cells |
| title_full_unstemmed | Essential Amino Acid Starvation-Induced Oxidative Stress Causes DNA Damage and Apoptosis in Murine Osteoblast-like Cells |
| title_short | Essential Amino Acid Starvation-Induced Oxidative Stress Causes DNA Damage and Apoptosis in Murine Osteoblast-like Cells |
| title_sort | essential amino acid starvation induced oxidative stress causes dna damage and apoptosis in murine osteoblast like cells |
| topic | essential amino acids apoptosis ROS osteoblasts |
| url | https://www.mdpi.com/1422-0067/24/20/15314 |
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