Rhizobium-Induced Calcium Spiking in Lotus japonicus
Legumes and rhizobium bacteria form a symbiosis that results in the development of nitrogen-fixing nodules on the root of the host plant. The earliest plant developmental changes are triggered by bacterially produced nodulation (Nod) factors. Within minutes of exposure to Nod factors, sharp oscillat...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2003-04-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2003.16.4.335 |
| _version_ | 1818914999635017728 |
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| author | Jeanne M. Harris Rebecca Wais Sharon R. Long |
| author_facet | Jeanne M. Harris Rebecca Wais Sharon R. Long |
| author_sort | Jeanne M. Harris |
| collection | DOAJ |
| description | Legumes and rhizobium bacteria form a symbiosis that results in the development of nitrogen-fixing nodules on the root of the host plant. The earliest plant developmental changes are triggered by bacterially produced nodulation (Nod) factors. Within minutes of exposure to Nod factors, sharp oscillations in cytoplasmic calcium levels (calcium spiking) occur in epidermal cells of several closely related legumes. We found that Lotus japonicus, a legume that follows an alternate developmental pathway, responds to both its bacterial partner and to the purified bacterial signal with calcium spiking. Thus, calcium spiking is not restricted to a particular pathway of nodule development and may be a general component of the response of host legumes to their bacterial partner. Using Nod factor-induced calcium spiking as a tool to identify mutants blocked early in the response to Nod factor, we show that the L. japonicus Ljsym22-1 mutant but not the Ljsym30 mutant fails to respond to Nod factor with calcium spiking. |
| first_indexed | 2024-12-19T23:55:18Z |
| format | Article |
| id | doaj.art-3aafe32787b744e0b501e6516056d38a |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:55:18Z |
| publishDate | 2003-04-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-3aafe32787b744e0b501e6516056d38a2022-12-21T20:01:01ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062003-04-0116433534110.1094/MPMI.2003.16.4.335Rhizobium-Induced Calcium Spiking in Lotus japonicusJeanne M. HarrisRebecca WaisSharon R. LongLegumes and rhizobium bacteria form a symbiosis that results in the development of nitrogen-fixing nodules on the root of the host plant. The earliest plant developmental changes are triggered by bacterially produced nodulation (Nod) factors. Within minutes of exposure to Nod factors, sharp oscillations in cytoplasmic calcium levels (calcium spiking) occur in epidermal cells of several closely related legumes. We found that Lotus japonicus, a legume that follows an alternate developmental pathway, responds to both its bacterial partner and to the purified bacterial signal with calcium spiking. Thus, calcium spiking is not restricted to a particular pathway of nodule development and may be a general component of the response of host legumes to their bacterial partner. Using Nod factor-induced calcium spiking as a tool to identify mutants blocked early in the response to Nod factor, we show that the L. japonicus Ljsym22-1 mutant but not the Ljsym30 mutant fails to respond to Nod factor with calcium spiking.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2003.16.4.335 |
| spellingShingle | Jeanne M. Harris Rebecca Wais Sharon R. Long Rhizobium-Induced Calcium Spiking in Lotus japonicus Molecular Plant-Microbe Interactions |
| title | Rhizobium-Induced Calcium Spiking in Lotus japonicus |
| title_full | Rhizobium-Induced Calcium Spiking in Lotus japonicus |
| title_fullStr | Rhizobium-Induced Calcium Spiking in Lotus japonicus |
| title_full_unstemmed | Rhizobium-Induced Calcium Spiking in Lotus japonicus |
| title_short | Rhizobium-Induced Calcium Spiking in Lotus japonicus |
| title_sort | rhizobium induced calcium spiking in lotus japonicus |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2003.16.4.335 |
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