TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice
Serine proteases are involved in many physiological activities as initiators of proteolytic cascades, and some members have been reported to play roles in male reproduction. Transmembrane serine protease 12 (TMPRSS12) has been shown to regulate sperm motility and uterotubal junction migration in mic...
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.757042/full |
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| author | Jingjing Zhang Jingjing Zhang Xinli Zhou Danyang Wan Li Yu Xu Chen Tong Yan Zhu Wu Meimei Zheng Meimei Zheng Feng Zhu Feng Zhu Hui Zhu |
| author_facet | Jingjing Zhang Jingjing Zhang Xinli Zhou Danyang Wan Li Yu Xu Chen Tong Yan Zhu Wu Meimei Zheng Meimei Zheng Feng Zhu Feng Zhu Hui Zhu |
| author_sort | Jingjing Zhang |
| collection | DOAJ |
| description | Serine proteases are involved in many physiological activities as initiators of proteolytic cascades, and some members have been reported to play roles in male reproduction. Transmembrane serine protease 12 (TMPRSS12) has been shown to regulate sperm motility and uterotubal junction migration in mice, but its role in the testis remains unknown. In this study, we verified that TMPRSS12 was expressed in the spermatocytes and spermatids of testis and the acrosome of sperm. Mice deficient in Tmprss12 exhibited male sterility. In meiosis, TMPRSS12 was demonstrated to regulate synapsis and double-strand break repair; spermatocytes of Tmprss12−/− mice underwent impaired meiosis and subsequent apoptosis, resulting in reduced sperm counts. During spermiogenesis, TMPRSS12 was found to function in the development of mitochondria; abnormal mitochondrial structure in Tmprss12−/− sperm led to reduced availability of ATP, impacting sperm motility. The differential protein expression profiles of testes in Tmprss12−/− and wild-type mice and further molecule identification revealed potential targets of TMPRSS12 related to meiosis and mitochondrial function. Besides, TMPRSS12 was also found to be involved in a series of sperm functions, including capacitation, acrosome reaction and sperm-egg interaction. These data imply that TMPRSS12 plays a role in multiple aspects of male reproduction. |
| first_indexed | 2024-12-10T09:58:39Z |
| format | Article |
| id | doaj.art-3ab0ab7500b44cf89ad76c456544b78d |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-10T09:58:39Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-3ab0ab7500b44cf89ad76c456544b78d2022-12-22T01:53:25ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-04-011010.3389/fcell.2022.757042757042TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in MiceJingjing Zhang0Jingjing Zhang1Xinli Zhou2Danyang Wan3Li Yu4Xu Chen5Tong Yan6Zhu Wu7Meimei Zheng8Meimei Zheng9Feng Zhu10Feng Zhu11Hui Zhu12State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaDepartment of Prenatal Diagnosis, Women’s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, ChinaState Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaState Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaState Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaState Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaState Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaState Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaState Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaReproductive Medicine Center of No. 960 Hospital of PLA, Jinan, ChinaState Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaDepartment of Pathology, The First People’s Hospital of Changzhou, Changzhou, ChinaState Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaSerine proteases are involved in many physiological activities as initiators of proteolytic cascades, and some members have been reported to play roles in male reproduction. Transmembrane serine protease 12 (TMPRSS12) has been shown to regulate sperm motility and uterotubal junction migration in mice, but its role in the testis remains unknown. In this study, we verified that TMPRSS12 was expressed in the spermatocytes and spermatids of testis and the acrosome of sperm. Mice deficient in Tmprss12 exhibited male sterility. In meiosis, TMPRSS12 was demonstrated to regulate synapsis and double-strand break repair; spermatocytes of Tmprss12−/− mice underwent impaired meiosis and subsequent apoptosis, resulting in reduced sperm counts. During spermiogenesis, TMPRSS12 was found to function in the development of mitochondria; abnormal mitochondrial structure in Tmprss12−/− sperm led to reduced availability of ATP, impacting sperm motility. The differential protein expression profiles of testes in Tmprss12−/− and wild-type mice and further molecule identification revealed potential targets of TMPRSS12 related to meiosis and mitochondrial function. Besides, TMPRSS12 was also found to be involved in a series of sperm functions, including capacitation, acrosome reaction and sperm-egg interaction. These data imply that TMPRSS12 plays a role in multiple aspects of male reproduction.https://www.frontiersin.org/articles/10.3389/fcell.2022.757042/fullTMPRSS12meiosisspermiogenesisserine proteasemale infertility |
| spellingShingle | Jingjing Zhang Jingjing Zhang Xinli Zhou Danyang Wan Li Yu Xu Chen Tong Yan Zhu Wu Meimei Zheng Meimei Zheng Feng Zhu Feng Zhu Hui Zhu TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice Frontiers in Cell and Developmental Biology TMPRSS12 meiosis spermiogenesis serine protease male infertility |
| title | TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice |
| title_full | TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice |
| title_fullStr | TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice |
| title_full_unstemmed | TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice |
| title_short | TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice |
| title_sort | tmprss12 functions in meiosis and spermiogenesis and is required for male fertility in mice |
| topic | TMPRSS12 meiosis spermiogenesis serine protease male infertility |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.757042/full |
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