Biophotonic Effects of Low-Level Laser Therapy at Different Wavelengths for Potential Wound Healing
Our objective was to assess the effect of low-level laser therapy (LLLT) administered using a diode laser on the growth processes of human fibroblast cells involved in wound healing. Initially, studies were conducted using a diode laser at wavelengths of 633, 520, and 450 nm with an irradiance of 3...
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MDPI AG
2022-08-01
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| Series: | Photonics |
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| Online Access: | https://www.mdpi.com/2304-6732/9/8/591 |
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| author | Tzu-Sen Yang Le-Thanh-Hang Nguyen Yu-Cheng Hsiao Li-Chern Pan Cheng-Jen Chang |
| author_facet | Tzu-Sen Yang Le-Thanh-Hang Nguyen Yu-Cheng Hsiao Li-Chern Pan Cheng-Jen Chang |
| author_sort | Tzu-Sen Yang |
| collection | DOAJ |
| description | Our objective was to assess the effect of low-level laser therapy (LLLT) administered using a diode laser on the growth processes of human fibroblast cells involved in wound healing. Initially, studies were conducted using a diode laser at wavelengths of 633, 520, and 450 nm with an irradiance of 3 mW/cm<sup>2</sup>. The distance between the light source and culture plate was 3 cm. The mechanism(s) of action of the diode laser illumination on human fibroblast cells were studied by examining different wavelengths to determine the relevant light parameters for optimal treatment. In addition, the percentages of fibroblast-mediated procollagen and matrix metallopeptidase (MMP)-1, -2, and -9 production were compared. In the clinical study, the changes in basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and fibroblast collagen production were assessed in 60 patients with complicated wounds who received LLLT (633 nm). No statistically significant difference was observed between red light versus green and blue light in the viability analysis. In addition, the effects of LLLT on the cell cultures of fibroblast cells in vitro demonstrated a decrease in the relative expression of MMP-1, -2, and -9 while using light with a wavelength of 633 nm. In the clinical study, 633 nm diode laser LLLT at 2–8 J/cm<sup>2</sup> was administered to 60 patients with complicated wounds; all patients showed increased levels of bFGF and VEGF and the occurrence of collagen synthesis. Our studies demonstrated that LLLT might affect fibroblast cell growth processes involved in wound healing. |
| first_indexed | 2024-03-09T03:56:22Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T03:56:22Z |
| publishDate | 2022-08-01 |
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| series | Photonics |
| spelling | doaj.art-3ab0f79cd8e24890b908c6149741509a2023-12-03T14:19:00ZengMDPI AGPhotonics2304-67322022-08-019859110.3390/photonics9080591Biophotonic Effects of Low-Level Laser Therapy at Different Wavelengths for Potential Wound HealingTzu-Sen Yang0Le-Thanh-Hang Nguyen1Yu-Cheng Hsiao2Li-Chern Pan3Cheng-Jen Chang4Graduate Institute of Biomedical Optomechatronics, Taipei Medical University, Taipei 110, TaiwanGraduate Institute of Biomedical Optomechatronics, Taipei Medical University, Taipei 110, TaiwanGraduate Institute of Biomedical Optomechatronics, Taipei Medical University, Taipei 110, TaiwanGraduate Institute of Biomedical Optomechatronics, Taipei Medical University, Taipei 110, TaiwanGraduate Institute of Biomedical Optomechatronics, Taipei Medical University, Taipei 110, TaiwanOur objective was to assess the effect of low-level laser therapy (LLLT) administered using a diode laser on the growth processes of human fibroblast cells involved in wound healing. Initially, studies were conducted using a diode laser at wavelengths of 633, 520, and 450 nm with an irradiance of 3 mW/cm<sup>2</sup>. The distance between the light source and culture plate was 3 cm. The mechanism(s) of action of the diode laser illumination on human fibroblast cells were studied by examining different wavelengths to determine the relevant light parameters for optimal treatment. In addition, the percentages of fibroblast-mediated procollagen and matrix metallopeptidase (MMP)-1, -2, and -9 production were compared. In the clinical study, the changes in basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and fibroblast collagen production were assessed in 60 patients with complicated wounds who received LLLT (633 nm). No statistically significant difference was observed between red light versus green and blue light in the viability analysis. In addition, the effects of LLLT on the cell cultures of fibroblast cells in vitro demonstrated a decrease in the relative expression of MMP-1, -2, and -9 while using light with a wavelength of 633 nm. In the clinical study, 633 nm diode laser LLLT at 2–8 J/cm<sup>2</sup> was administered to 60 patients with complicated wounds; all patients showed increased levels of bFGF and VEGF and the occurrence of collagen synthesis. Our studies demonstrated that LLLT might affect fibroblast cell growth processes involved in wound healing.https://www.mdpi.com/2304-6732/9/8/591low-level laser therapydiode laserfibroblast cellsmatrix metallopeptidasewound healing |
| spellingShingle | Tzu-Sen Yang Le-Thanh-Hang Nguyen Yu-Cheng Hsiao Li-Chern Pan Cheng-Jen Chang Biophotonic Effects of Low-Level Laser Therapy at Different Wavelengths for Potential Wound Healing Photonics low-level laser therapy diode laser fibroblast cells matrix metallopeptidase wound healing |
| title | Biophotonic Effects of Low-Level Laser Therapy at Different Wavelengths for Potential Wound Healing |
| title_full | Biophotonic Effects of Low-Level Laser Therapy at Different Wavelengths for Potential Wound Healing |
| title_fullStr | Biophotonic Effects of Low-Level Laser Therapy at Different Wavelengths for Potential Wound Healing |
| title_full_unstemmed | Biophotonic Effects of Low-Level Laser Therapy at Different Wavelengths for Potential Wound Healing |
| title_short | Biophotonic Effects of Low-Level Laser Therapy at Different Wavelengths for Potential Wound Healing |
| title_sort | biophotonic effects of low level laser therapy at different wavelengths for potential wound healing |
| topic | low-level laser therapy diode laser fibroblast cells matrix metallopeptidase wound healing |
| url | https://www.mdpi.com/2304-6732/9/8/591 |
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