| Summary: | The purpose of the study was to reach high efficiency of an oil-degrading bacteria to be used in oil-spill treatment separately or with bacterial consortium. The consortium effect on crude oil was examined by GC-MS. The consortium degraded 99.2% of crude oil after 7 days, while Enterobacter sp. ASH, as individual culture, degraded 80% only. This isolate was identified based on 16S rRNA gene sequence analysis, morphological, physiological, and biochemical characterization. ANOVA analysis showed that medium volume and crude oil concentration are the significant factors. The results showed that the mixed consortium showed better biodegradation abilities than E. sp. ASH.
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