Quality control method of Polygala telephioides herba
In order to control the quality of Polygala telephioides herba, a method is established for the qualitative and quantitative analysis of the herba. Firstly, a TLC method is developed for qualitative detection of Polygala telephioides. Then, with home-made Telephenone B as comparison product and Mang...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
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Hebei University of Science and Technology
2016-12-01
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| Series: | Journal of Hebei University of Science and Technology |
| Subjects: | |
| Online Access: | http://xuebao.hebust.edu.cn/hbkjdx/ch/reader/create_pdf.aspx?file_no=b201606009&flag=1&journal_ |
| _version_ | 1819112661527298048 |
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| author | Jianchen LI Yun RAN Haifeng ZHAI Jing HA Zhiqing DUAN |
| author_facet | Jianchen LI Yun RAN Haifeng ZHAI Jing HA Zhiqing DUAN |
| author_sort | Jianchen LI |
| collection | DOAJ |
| description | In order to control the quality of Polygala telephioides herba, a method is established for the qualitative and quantitative analysis of the herba. Firstly, a TLC method is developed for qualitative detection of Polygala telephioides. Then, with home-made Telephenone B as comparison product and Mangiferin as the reference substance, the relative correlation factor (RCF) of Telephenone B and Mangiferin is determined. The contents are determined by HPLC combined with the quantitative analysis of multi-components by single-marker (QAMS), and the calculated value of QAMS is compared with the measured result to verify the veracity and feasibility of the QAMS method. The results show that the TLC spots are clear and easy to judge. Mangiferin and Telephenone B show good linear relationship in the range of 0.2~1.4 μg (r=0.999 9), 0.13~0.91 μg (r=0.999 4), respectively. The established relative correlation factor shows good reproducibility, and there is no significant difference between the content result calculated by RCF and the content result determined by external standard method. This method is accurate, feasible, and can be used for the quality control of Polygala telephioides herba. |
| first_indexed | 2024-12-22T04:17:03Z |
| format | Article |
| id | doaj.art-3ab8917ba004488d9f9e2512d4266d8b |
| institution | Directory Open Access Journal |
| issn | 1008-1542 |
| language | zho |
| last_indexed | 2024-12-22T04:17:03Z |
| publishDate | 2016-12-01 |
| publisher | Hebei University of Science and Technology |
| record_format | Article |
| series | Journal of Hebei University of Science and Technology |
| spelling | doaj.art-3ab8917ba004488d9f9e2512d4266d8b2022-12-21T18:39:23ZzhoHebei University of Science and TechnologyJournal of Hebei University of Science and Technology1008-15422016-12-0137658158610.7535/hbkd.2016yx06009b201606009Quality control method of Polygala telephioides herbaJianchen LI0Yun RAN1Haifeng ZHAI2Jing HA3Zhiqing DUAN4School of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, ChinaSchool of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, ChinaNational Institute on Drug Dependence, Peking University, Beijing 100191, ChinaSchool of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, ChinaThe First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, ChinaIn order to control the quality of Polygala telephioides herba, a method is established for the qualitative and quantitative analysis of the herba. Firstly, a TLC method is developed for qualitative detection of Polygala telephioides. Then, with home-made Telephenone B as comparison product and Mangiferin as the reference substance, the relative correlation factor (RCF) of Telephenone B and Mangiferin is determined. The contents are determined by HPLC combined with the quantitative analysis of multi-components by single-marker (QAMS), and the calculated value of QAMS is compared with the measured result to verify the veracity and feasibility of the QAMS method. The results show that the TLC spots are clear and easy to judge. Mangiferin and Telephenone B show good linear relationship in the range of 0.2~1.4 μg (r=0.999 9), 0.13~0.91 μg (r=0.999 4), respectively. The established relative correlation factor shows good reproducibility, and there is no significant difference between the content result calculated by RCF and the content result determined by external standard method. This method is accurate, feasible, and can be used for the quality control of Polygala telephioides herba.http://xuebao.hebust.edu.cn/hbkjdx/ch/reader/create_pdf.aspx?file_no=b201606009&flag=1&journal_Chinese medicine chemistryPolygala telephioides herbaquality controlTLCquantitative analysis of multi-components by single-marker (QAMS)relative correlation factor (RCF) |
| spellingShingle | Jianchen LI Yun RAN Haifeng ZHAI Jing HA Zhiqing DUAN Quality control method of Polygala telephioides herba Journal of Hebei University of Science and Technology Chinese medicine chemistry Polygala telephioides herba quality control TLC quantitative analysis of multi-components by single-marker (QAMS) relative correlation factor (RCF) |
| title | Quality control method of Polygala telephioides herba |
| title_full | Quality control method of Polygala telephioides herba |
| title_fullStr | Quality control method of Polygala telephioides herba |
| title_full_unstemmed | Quality control method of Polygala telephioides herba |
| title_short | Quality control method of Polygala telephioides herba |
| title_sort | quality control method of polygala telephioides herba |
| topic | Chinese medicine chemistry Polygala telephioides herba quality control TLC quantitative analysis of multi-components by single-marker (QAMS) relative correlation factor (RCF) |
| url | http://xuebao.hebust.edu.cn/hbkjdx/ch/reader/create_pdf.aspx?file_no=b201606009&flag=1&journal_ |
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