LncRNA MINCR inhibits LPS-induced damage and inflammation of alveolar epithelial cell line A549 by targeting at miR-223
Objective To investigate the potential effects of long non-coding RNA (lncRNA) MINCR on lipopolysaccharide (LPS)-induced human alveolar epithelial cell line A549 damage and inflammation through targeted regulation of miR-223. Methods A549 cells were randomly divided into control group, LPS group, LP...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2023-01-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-1-102.pdf |
| _version_ | 1797366337844019200 |
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| author | YANG Guanglin, REN Lijuan, CHEN Wenjing, XIONG Weijun |
| author_facet | YANG Guanglin, REN Lijuan, CHEN Wenjing, XIONG Weijun |
| author_sort | YANG Guanglin, REN Lijuan, CHEN Wenjing, XIONG Weijun |
| collection | DOAJ |
| description | Objective To investigate the potential effects of long non-coding RNA (lncRNA) MINCR on lipopolysaccharide (LPS)-induced human alveolar epithelial cell line A549 damage and inflammation through targeted regulation of miR-223. Methods A549 cells were randomly divided into control group, LPS group, LPS+transfection group(Si NC group, Si MINCR group, miR-223 NC group, miR-223 mimic group, Si NC+miR-223 NC group, Si NC+miR-223 antagomir group, Si MINCR+miR-223 NC group, and Si MINCR+miR-223 antagomir group). RT-qPCR was performed to determine the expression levels of MINCR and miR-223; Flow cytometry was performed to determine the rate of apoptosis; ELISA was performed to determine the levels of serum tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6); Bioinformatics prediction and dual luciferase reporter gene assay were performed to verify the targeting relationship of MINCR and miR-223; Western blot was performed to test the expression levels of activated caspase-3 (cleaved caspase-3) and B-cell lymphoma-2(Bcl-2) proteins in the cells. Results There was a targeting relationship between MINCR and miR-223. Compared with the control group, the apoptosis rate, the level of TNF-α and IL-6, and the expression of MINCR, cleaved caspase-3 proteins in the LPS group were significantly increased (PPPPPPPConclusions Silencing the expression of lncRNA MINCR may target the activation of miR-223 expression, inhibit cell apoptosis and inflammation, and reduce LPS-induced damage of alveolar epithelial cells. |
| first_indexed | 2024-03-08T17:02:48Z |
| format | Article |
| id | doaj.art-3ababa5a77af462fb5b3cc721826716b |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T17:02:48Z |
| publishDate | 2023-01-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-3ababa5a77af462fb5b3cc721826716b2024-01-04T07:27:26ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252023-01-0143110210910.16352/j.issn.1001-6325.2023.01.0102LncRNA MINCR inhibits LPS-induced damage and inflammation of alveolar epithelial cell line A549 by targeting at miR-223YANG Guanglin, REN Lijuan, CHEN Wenjing, XIONG Weijun0Department of Respiratory Medicine, Affiliated Hospital of Sichuan Nursing Vocational College/ the Third People's Hospital of Sichuan Province, Chengdu 610100, ChinaObjective To investigate the potential effects of long non-coding RNA (lncRNA) MINCR on lipopolysaccharide (LPS)-induced human alveolar epithelial cell line A549 damage and inflammation through targeted regulation of miR-223. Methods A549 cells were randomly divided into control group, LPS group, LPS+transfection group(Si NC group, Si MINCR group, miR-223 NC group, miR-223 mimic group, Si NC+miR-223 NC group, Si NC+miR-223 antagomir group, Si MINCR+miR-223 NC group, and Si MINCR+miR-223 antagomir group). RT-qPCR was performed to determine the expression levels of MINCR and miR-223; Flow cytometry was performed to determine the rate of apoptosis; ELISA was performed to determine the levels of serum tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6); Bioinformatics prediction and dual luciferase reporter gene assay were performed to verify the targeting relationship of MINCR and miR-223; Western blot was performed to test the expression levels of activated caspase-3 (cleaved caspase-3) and B-cell lymphoma-2(Bcl-2) proteins in the cells. Results There was a targeting relationship between MINCR and miR-223. Compared with the control group, the apoptosis rate, the level of TNF-α and IL-6, and the expression of MINCR, cleaved caspase-3 proteins in the LPS group were significantly increased (PPPPPPPConclusions Silencing the expression of lncRNA MINCR may target the activation of miR-223 expression, inhibit cell apoptosis and inflammation, and reduce LPS-induced damage of alveolar epithelial cells.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-1-102.pdfalveolar epithelial cells|lncrna mincr|mir-223|inflammation|cell damage |
| spellingShingle | YANG Guanglin, REN Lijuan, CHEN Wenjing, XIONG Weijun LncRNA MINCR inhibits LPS-induced damage and inflammation of alveolar epithelial cell line A549 by targeting at miR-223 Jichu yixue yu linchuang alveolar epithelial cells|lncrna mincr|mir-223|inflammation|cell damage |
| title | LncRNA MINCR inhibits LPS-induced damage and inflammation of alveolar epithelial cell line A549 by targeting at miR-223 |
| title_full | LncRNA MINCR inhibits LPS-induced damage and inflammation of alveolar epithelial cell line A549 by targeting at miR-223 |
| title_fullStr | LncRNA MINCR inhibits LPS-induced damage and inflammation of alveolar epithelial cell line A549 by targeting at miR-223 |
| title_full_unstemmed | LncRNA MINCR inhibits LPS-induced damage and inflammation of alveolar epithelial cell line A549 by targeting at miR-223 |
| title_short | LncRNA MINCR inhibits LPS-induced damage and inflammation of alveolar epithelial cell line A549 by targeting at miR-223 |
| title_sort | lncrna mincr inhibits lps induced damage and inflammation of alveolar epithelial cell line a549 by targeting at mir 223 |
| topic | alveolar epithelial cells|lncrna mincr|mir-223|inflammation|cell damage |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-1-102.pdf |
| work_keys_str_mv | AT yangguanglinrenlijuanchenwenjingxiongweijun lncrnamincrinhibitslpsinduceddamageandinflammationofalveolarepithelialcelllinea549bytargetingatmir223 |