Role of laminin bioavailability in the astroglial permissivity for neuritic outgrowth
The mechanisms involved in the failure of an adult brain to regenerate post-lesion remain poorly understood. The reactive gliosis which occurs after an injury to the CNS and leads to the glial scar has been considered as one of the major impediments to neurite outgrowth and axonal regeneration. A gl...
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Format: | Article |
Language: | English |
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Academia Brasileira de Ciências
2002-01-01
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Series: | Anais da Academia Brasileira de Ciências |
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000400009 |
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author | TARDY MARCIENNE |
author_facet | TARDY MARCIENNE |
author_sort | TARDY MARCIENNE |
collection | DOAJ |
description | The mechanisms involved in the failure of an adult brain to regenerate post-lesion remain poorly understood. The reactive gliosis which occurs after an injury to the CNS and leads to the glial scar has been considered as one of the major impediments to neurite outgrowth and axonal regeneration. A glial scar consists mainly of reactive, hypertrophic astrocytes. These reactive cells acquire new properties, leading to A non-permissive support for neurons. Astrogial reactivity is mainly characteriized by a high overexpression of the major component of the gliofilaments, the glial fibrillary acidic protein (GFAP). This GFAP overexpression is related to the astroglial morphological response to injury. We hypothesized that modulation of GFAP synthesis, reversing the hypertrophic phenotype, might also reverse the blockage of neuritic outgrowth observed after a lesion. In this article, we review findings of our group, confirming our hypothesis in a model of lesioned neuron-astrocyte cocultures. We demonstrate that permissivity for neuritic outgrowth is related to phenotypic changes induced in reactive astrocytes transfected by antisense GFAP-mRNA. We also found that this permissivity was related to a neuron-regulated extracellular laminin bioavailability. |
first_indexed | 2024-12-23T19:14:14Z |
format | Article |
id | doaj.art-3abb8b61be3747d9980e92c0f785efb9 |
institution | Directory Open Access Journal |
issn | 0001-3765 1678-2690 |
language | English |
last_indexed | 2024-12-23T19:14:14Z |
publishDate | 2002-01-01 |
publisher | Academia Brasileira de Ciências |
record_format | Article |
series | Anais da Academia Brasileira de Ciências |
spelling | doaj.art-3abb8b61be3747d9980e92c0f785efb92022-12-21T17:34:21ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências0001-37651678-26902002-01-01744683690Role of laminin bioavailability in the astroglial permissivity for neuritic outgrowthTARDY MARCIENNEThe mechanisms involved in the failure of an adult brain to regenerate post-lesion remain poorly understood. The reactive gliosis which occurs after an injury to the CNS and leads to the glial scar has been considered as one of the major impediments to neurite outgrowth and axonal regeneration. A glial scar consists mainly of reactive, hypertrophic astrocytes. These reactive cells acquire new properties, leading to A non-permissive support for neurons. Astrogial reactivity is mainly characteriized by a high overexpression of the major component of the gliofilaments, the glial fibrillary acidic protein (GFAP). This GFAP overexpression is related to the astroglial morphological response to injury. We hypothesized that modulation of GFAP synthesis, reversing the hypertrophic phenotype, might also reverse the blockage of neuritic outgrowth observed after a lesion. In this article, we review findings of our group, confirming our hypothesis in a model of lesioned neuron-astrocyte cocultures. We demonstrate that permissivity for neuritic outgrowth is related to phenotypic changes induced in reactive astrocytes transfected by antisense GFAP-mRNA. We also found that this permissivity was related to a neuron-regulated extracellular laminin bioavailability.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000400009astrogliosisneuro-glia interactionsneurite outgrowthneuronal migrationlamininmetalloproteinases |
spellingShingle | TARDY MARCIENNE Role of laminin bioavailability in the astroglial permissivity for neuritic outgrowth Anais da Academia Brasileira de Ciências astrogliosis neuro-glia interactions neurite outgrowth neuronal migration laminin metalloproteinases |
title | Role of laminin bioavailability in the astroglial permissivity for neuritic outgrowth |
title_full | Role of laminin bioavailability in the astroglial permissivity for neuritic outgrowth |
title_fullStr | Role of laminin bioavailability in the astroglial permissivity for neuritic outgrowth |
title_full_unstemmed | Role of laminin bioavailability in the astroglial permissivity for neuritic outgrowth |
title_short | Role of laminin bioavailability in the astroglial permissivity for neuritic outgrowth |
title_sort | role of laminin bioavailability in the astroglial permissivity for neuritic outgrowth |
topic | astrogliosis neuro-glia interactions neurite outgrowth neuronal migration laminin metalloproteinases |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000400009 |
work_keys_str_mv | AT tardymarcienne roleoflamininbioavailabilityintheastroglialpermissivityforneuriticoutgrowth |