Astrocytes exhibit diverse Ca2+ changes at subcellular domains during brain aging
Astrocytic Ca2+ transients are essential for astrocyte integration into neural circuits. These Ca2+ transients are primarily sequestered in subcellular domains, including primary branches, branchlets and leaflets, and endfeet. In previous studies, it suggests that aging causes functional defects in...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Aging Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnagi.2022.1029533/full |
| _version_ | 1811197402818805760 |
|---|---|
| author | Fusheng Ding Fusheng Ding Fusheng Ding Shanshan Liang Ruijie Li Ruijie Li Zhiqi Yang Yong He Shaofan Yang Qingtian Duan Jianxiong Zhang Jing Lyu Zhenqiao Zhou Mingzhu Huang Haoyu Wang Jin Li Chuanyan Yang Yuxia Wang Mingyue Gong Shangbin Chen Shangbin Chen Hongbo Jia Hongbo Jia Hongbo Jia Xiaowei Chen Xiaowei Chen Xiang Liao Ling Fu Ling Fu Kuan Zhang |
| author_facet | Fusheng Ding Fusheng Ding Fusheng Ding Shanshan Liang Ruijie Li Ruijie Li Zhiqi Yang Yong He Shaofan Yang Qingtian Duan Jianxiong Zhang Jing Lyu Zhenqiao Zhou Mingzhu Huang Haoyu Wang Jin Li Chuanyan Yang Yuxia Wang Mingyue Gong Shangbin Chen Shangbin Chen Hongbo Jia Hongbo Jia Hongbo Jia Xiaowei Chen Xiaowei Chen Xiang Liao Ling Fu Ling Fu Kuan Zhang |
| author_sort | Fusheng Ding |
| collection | DOAJ |
| description | Astrocytic Ca2+ transients are essential for astrocyte integration into neural circuits. These Ca2+ transients are primarily sequestered in subcellular domains, including primary branches, branchlets and leaflets, and endfeet. In previous studies, it suggests that aging causes functional defects in astrocytes. Until now, it was unclear whether and how aging affects astrocytic Ca2+ transients at subcellular domains. In this study, we combined a genetically encoded Ca2+ sensor (GCaMP6f) and in vivo two-photon Ca2+ imaging to determine changes in Ca2+ transients within astrocytic subcellular domains during brain aging. We showed that aging increased Ca2+ transients in astrocytic primary branches, higher-order branchlets, and terminal leaflets. However, Ca2+ transients decreased within astrocytic endfeet during brain aging, which could be caused by the decreased expressions of Aquaporin-4 (AQP4). In addition, aging-induced changes of Ca2+ transient types were heterogeneous within astrocytic subcellular domains. These results demonstrate that the astrocytic Ca2+ transients within subcellular domains are affected by aging differently. This finding contributes to a better understanding of the physiological role of astrocytes in aging-induced neural circuit degeneration. |
| first_indexed | 2024-04-12T01:14:11Z |
| format | Article |
| id | doaj.art-3ac07154c12645a4a38f5a9c6aa2150e |
| institution | Directory Open Access Journal |
| issn | 1663-4365 |
| language | English |
| last_indexed | 2024-04-12T01:14:11Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Aging Neuroscience |
| spelling | doaj.art-3ac07154c12645a4a38f5a9c6aa2150e2022-12-22T03:54:01ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652022-10-011410.3389/fnagi.2022.10295331029533Astrocytes exhibit diverse Ca2+ changes at subcellular domains during brain agingFusheng Ding0Fusheng Ding1Fusheng Ding2Shanshan Liang3Ruijie Li4Ruijie Li5Zhiqi Yang6Yong He7Shaofan Yang8Qingtian Duan9Jianxiong Zhang10Jing Lyu11Zhenqiao Zhou12Mingzhu Huang13Haoyu Wang14Jin Li15Chuanyan Yang16Yuxia Wang17Mingyue Gong18Shangbin Chen19Shangbin Chen20Hongbo Jia21Hongbo Jia22Hongbo Jia23Xiaowei Chen24Xiaowei Chen25Xiang Liao26Ling Fu27Ling Fu28Kuan Zhang29Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, ChinaMoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaAdvanced Institute for Brain and Intelligence and School of Physical Science and Technology, Guangxi University, Nanning, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaBrain Research Instrument Innovation Center, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, ChinaBrain Research Instrument Innovation Center, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, ChinaMoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaAdvanced Institute for Brain and Intelligence and School of Physical Science and Technology, Guangxi University, Nanning, ChinaBrain Research Instrument Innovation Center, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, ChinaCombinatorial NeuroImaging Core Facility, Leibniz Institute for Neurobiology, Magdeburg, GermanyBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaGuangyang Bay Laboratory, Chongqing Institute for Brain and Intelligence, Chongqing, ChinaCenter for Neurointelligence, School of Medicine, Chongqing University, Chongqing, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, ChinaMoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaAstrocytic Ca2+ transients are essential for astrocyte integration into neural circuits. These Ca2+ transients are primarily sequestered in subcellular domains, including primary branches, branchlets and leaflets, and endfeet. In previous studies, it suggests that aging causes functional defects in astrocytes. Until now, it was unclear whether and how aging affects astrocytic Ca2+ transients at subcellular domains. In this study, we combined a genetically encoded Ca2+ sensor (GCaMP6f) and in vivo two-photon Ca2+ imaging to determine changes in Ca2+ transients within astrocytic subcellular domains during brain aging. We showed that aging increased Ca2+ transients in astrocytic primary branches, higher-order branchlets, and terminal leaflets. However, Ca2+ transients decreased within astrocytic endfeet during brain aging, which could be caused by the decreased expressions of Aquaporin-4 (AQP4). In addition, aging-induced changes of Ca2+ transient types were heterogeneous within astrocytic subcellular domains. These results demonstrate that the astrocytic Ca2+ transients within subcellular domains are affected by aging differently. This finding contributes to a better understanding of the physiological role of astrocytes in aging-induced neural circuit degeneration.https://www.frontiersin.org/articles/10.3389/fnagi.2022.1029533/fullastrocyteCa2+ transientsbranchesbranchlets and leafletsendfeetaging |
| spellingShingle | Fusheng Ding Fusheng Ding Fusheng Ding Shanshan Liang Ruijie Li Ruijie Li Zhiqi Yang Yong He Shaofan Yang Qingtian Duan Jianxiong Zhang Jing Lyu Zhenqiao Zhou Mingzhu Huang Haoyu Wang Jin Li Chuanyan Yang Yuxia Wang Mingyue Gong Shangbin Chen Shangbin Chen Hongbo Jia Hongbo Jia Hongbo Jia Xiaowei Chen Xiaowei Chen Xiang Liao Ling Fu Ling Fu Kuan Zhang Astrocytes exhibit diverse Ca2+ changes at subcellular domains during brain aging Frontiers in Aging Neuroscience astrocyte Ca2+ transients branches branchlets and leaflets endfeet aging |
| title | Astrocytes exhibit diverse Ca2+ changes at subcellular domains during brain aging |
| title_full | Astrocytes exhibit diverse Ca2+ changes at subcellular domains during brain aging |
| title_fullStr | Astrocytes exhibit diverse Ca2+ changes at subcellular domains during brain aging |
| title_full_unstemmed | Astrocytes exhibit diverse Ca2+ changes at subcellular domains during brain aging |
| title_short | Astrocytes exhibit diverse Ca2+ changes at subcellular domains during brain aging |
| title_sort | astrocytes exhibit diverse ca2 changes at subcellular domains during brain aging |
| topic | astrocyte Ca2+ transients branches branchlets and leaflets endfeet aging |
| url | https://www.frontiersin.org/articles/10.3389/fnagi.2022.1029533/full |
| work_keys_str_mv | AT fushengding astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT fushengding astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT fushengding astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT shanshanliang astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT ruijieli astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT ruijieli astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT zhiqiyang astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT yonghe astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT shaofanyang astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT qingtianduan astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT jianxiongzhang astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT jinglyu astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT zhenqiaozhou astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT mingzhuhuang astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT haoyuwang astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT jinli astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT chuanyanyang astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT yuxiawang astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT mingyuegong astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT shangbinchen astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT shangbinchen astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT hongbojia astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT hongbojia astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT hongbojia astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT xiaoweichen astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT xiaoweichen astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT xiangliao astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT lingfu astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT lingfu astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging AT kuanzhang astrocytesexhibitdiverseca2changesatsubcellulardomainsduringbrainaging |