Cold stress responsive microRNAs and their targets in Musa balbisiana
Cold stress is an environmental factor affecting plant development and production. Recently, microRNAs (miRNAs) have been found to be involved in several plant processes such as growth regulation and stress responses. Although miRNAs and their targets have been identified in several banana species,...
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| Format: | Article |
| Language: | English |
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Higher Education Press
2016-12-01
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| Series: | Frontiers of Agricultural Science and Engineering |
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| Online Access: | http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/1481680415799-1938768405.pdf |
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| author | Jingyi WANG,Juhua LIU,Caihong JIA,Hongxia MIAO,Jianbin ZHANG,Zhuo WANG,Biyu XU,Zhiqiang JIN |
| author_facet | Jingyi WANG,Juhua LIU,Caihong JIA,Hongxia MIAO,Jianbin ZHANG,Zhuo WANG,Biyu XU,Zhiqiang JIN |
| author_sort | Jingyi WANG,Juhua LIU,Caihong JIA,Hongxia MIAO,Jianbin ZHANG,Zhuo WANG,Biyu XU,Zhiqiang JIN |
| collection | DOAJ |
| description | Cold stress is an environmental factor affecting plant development and production. Recently, microRNAs (miRNAs) have been found to be involved in several plant processes such as growth regulation and stress responses. Although miRNAs and their targets have been identified in several banana species, their participation during cold accumulation in banana remains unknown. In this study, two small RNA libraries were generated from micropropagated plantlets of Musa balbisiana grown at normal and low temperature (5°C). A total of 69 known miRNAs and 32 putative novel miRNAs were detected in the libraries by Solexa sequencing. Sixty-four cold-inducible miRNAs were identified through differentially expressed miRNAs analysis. Among 43 miRNAs belonging to 26 conserved miRNA families with altered expression, 18 were upregulated and 25 downregulated under cold stress. Of 21 putative novel miRNAs with altered expression, four were downregulated and 17 upregulated. Furthermore, eight miRNAs were validated by stem-loop qRT-PCR and their dynamic differential expression was analyzed. In addition, 393 target genes of 58 identified cold-responsive miRNAs were predicted and categorized by function. These results provide important information for further characterization and functional analysis of cold-responsive miRNAs in banana. |
| first_indexed | 2024-12-21T01:42:02Z |
| format | Article |
| id | doaj.art-3ac1f7569b7d413f88972736fc24c47f |
| institution | Directory Open Access Journal |
| issn | 2095-7505 |
| language | English |
| last_indexed | 2024-12-21T01:42:02Z |
| publishDate | 2016-12-01 |
| publisher | Higher Education Press |
| record_format | Article |
| series | Frontiers of Agricultural Science and Engineering |
| spelling | doaj.art-3ac1f7569b7d413f88972736fc24c47f2022-12-21T19:20:07ZengHigher Education PressFrontiers of Agricultural Science and Engineering2095-75052016-12-013433534510.15302/J-FASE-2016121Cold stress responsive microRNAs and their targets in Musa balbisianaJingyi WANG,Juhua LIU,Caihong JIA,Hongxia MIAO,Jianbin ZHANG,Zhuo WANG,Biyu XU,Zhiqiang JIN01. Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; 2. Key Laboratory of Genetic Improvement of Bananas, Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou 570101, ChinaCold stress is an environmental factor affecting plant development and production. Recently, microRNAs (miRNAs) have been found to be involved in several plant processes such as growth regulation and stress responses. Although miRNAs and their targets have been identified in several banana species, their participation during cold accumulation in banana remains unknown. In this study, two small RNA libraries were generated from micropropagated plantlets of Musa balbisiana grown at normal and low temperature (5°C). A total of 69 known miRNAs and 32 putative novel miRNAs were detected in the libraries by Solexa sequencing. Sixty-four cold-inducible miRNAs were identified through differentially expressed miRNAs analysis. Among 43 miRNAs belonging to 26 conserved miRNA families with altered expression, 18 were upregulated and 25 downregulated under cold stress. Of 21 putative novel miRNAs with altered expression, four were downregulated and 17 upregulated. Furthermore, eight miRNAs were validated by stem-loop qRT-PCR and their dynamic differential expression was analyzed. In addition, 393 target genes of 58 identified cold-responsive miRNAs were predicted and categorized by function. These results provide important information for further characterization and functional analysis of cold-responsive miRNAs in banana.http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/1481680415799-1938768405.pdfcold stress|microRNA|Musa balbisiana |
| spellingShingle | Jingyi WANG,Juhua LIU,Caihong JIA,Hongxia MIAO,Jianbin ZHANG,Zhuo WANG,Biyu XU,Zhiqiang JIN Cold stress responsive microRNAs and their targets in Musa balbisiana Frontiers of Agricultural Science and Engineering cold stress|microRNA|Musa balbisiana |
| title | Cold stress responsive microRNAs and their targets in Musa balbisiana |
| title_full | Cold stress responsive microRNAs and their targets in Musa balbisiana |
| title_fullStr | Cold stress responsive microRNAs and their targets in Musa balbisiana |
| title_full_unstemmed | Cold stress responsive microRNAs and their targets in Musa balbisiana |
| title_short | Cold stress responsive microRNAs and their targets in Musa balbisiana |
| title_sort | cold stress responsive micrornas and their targets in musa balbisiana |
| topic | cold stress|microRNA|Musa balbisiana |
| url | http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/1481680415799-1938768405.pdf |
| work_keys_str_mv | AT jingyiwangjuhualiucaihongjiahongxiamiaojianbinzhangzhuowangbiyuxuzhiqiangjin coldstressresponsivemicrornasandtheirtargetsinmusabalbisiana |