Construction of ultra-high-density genetic linkage map of a sorghum-sudangrass hybrid using whole genome resequencing
The sorghum-sudangrass hybrid is a vital annual gramineous herbage. Few reports exist on its ultra-high-density genetic map. In this study, we sought to create an ultra-high-density genetic linkage map for this hybrid to strengthen its functional genomics research and genetic breeding. We used 150 s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9707794/?tool=EBI |
| _version_ | 1811178436392124416 |
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| author | Qianqian Lu Xiaoxia Yu Huiting Wang Zhuo Yu Xia Zhang Yaqi Zhao |
| author_facet | Qianqian Lu Xiaoxia Yu Huiting Wang Zhuo Yu Xia Zhang Yaqi Zhao |
| author_sort | Qianqian Lu |
| collection | DOAJ |
| description | The sorghum-sudangrass hybrid is a vital annual gramineous herbage. Few reports exist on its ultra-high-density genetic map. In this study, we sought to create an ultra-high-density genetic linkage map for this hybrid to strengthen its functional genomics research and genetic breeding. We used 150 sorghum-sudangrass hybrid F2 individuals and their parents (scattered ear sorghum and red hull sudangrass) for high-throughput sequencing on the basis of whole genome resequencing. In total, 1,180.66 Gb of data were collected. After identification, filtration for integrity, and partial segregation, over 5,656 single nucleotide polymorphism markers of high quality were detected. An ultra-high-density genetic linkage map was constructed using these data. The markers covered approximately 2,192.84 cM of the map with average marker intervals of 0.39 cM. The length ranged from 115.39 cM to 264.04 cM for the 10 linkage groups. Currently, this represents the first genetic linkage map of this size, number of molecular markers, density, and coverage for sorghum-sudangrass hybrid. The findings of this study provide valuable genome-level information on species evolution and comparative genomics analysis and lay the foundation for further research on quantitative trait loci fine mapping and gene cloning and marker-assisted breeding of important traits in sorghum-sudangrass hybrids. |
| first_indexed | 2024-04-11T06:19:23Z |
| format | Article |
| id | doaj.art-3ac21791af7c4d24a8dea5e55098c0f6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T06:19:23Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3ac21791af7c4d24a8dea5e55098c0f62022-12-22T04:40:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011711Construction of ultra-high-density genetic linkage map of a sorghum-sudangrass hybrid using whole genome resequencingQianqian LuXiaoxia YuHuiting WangZhuo YuXia ZhangYaqi ZhaoThe sorghum-sudangrass hybrid is a vital annual gramineous herbage. Few reports exist on its ultra-high-density genetic map. In this study, we sought to create an ultra-high-density genetic linkage map for this hybrid to strengthen its functional genomics research and genetic breeding. We used 150 sorghum-sudangrass hybrid F2 individuals and their parents (scattered ear sorghum and red hull sudangrass) for high-throughput sequencing on the basis of whole genome resequencing. In total, 1,180.66 Gb of data were collected. After identification, filtration for integrity, and partial segregation, over 5,656 single nucleotide polymorphism markers of high quality were detected. An ultra-high-density genetic linkage map was constructed using these data. The markers covered approximately 2,192.84 cM of the map with average marker intervals of 0.39 cM. The length ranged from 115.39 cM to 264.04 cM for the 10 linkage groups. Currently, this represents the first genetic linkage map of this size, number of molecular markers, density, and coverage for sorghum-sudangrass hybrid. The findings of this study provide valuable genome-level information on species evolution and comparative genomics analysis and lay the foundation for further research on quantitative trait loci fine mapping and gene cloning and marker-assisted breeding of important traits in sorghum-sudangrass hybrids.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9707794/?tool=EBI |
| spellingShingle | Qianqian Lu Xiaoxia Yu Huiting Wang Zhuo Yu Xia Zhang Yaqi Zhao Construction of ultra-high-density genetic linkage map of a sorghum-sudangrass hybrid using whole genome resequencing PLoS ONE |
| title | Construction of ultra-high-density genetic linkage map of a sorghum-sudangrass hybrid using whole genome resequencing |
| title_full | Construction of ultra-high-density genetic linkage map of a sorghum-sudangrass hybrid using whole genome resequencing |
| title_fullStr | Construction of ultra-high-density genetic linkage map of a sorghum-sudangrass hybrid using whole genome resequencing |
| title_full_unstemmed | Construction of ultra-high-density genetic linkage map of a sorghum-sudangrass hybrid using whole genome resequencing |
| title_short | Construction of ultra-high-density genetic linkage map of a sorghum-sudangrass hybrid using whole genome resequencing |
| title_sort | construction of ultra high density genetic linkage map of a sorghum sudangrass hybrid using whole genome resequencing |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9707794/?tool=EBI |
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