| Summary: | Diacylglycerol acyltransferase (DGAT) is a vital and sole rate-limiting enzyme involved in triacylglycerol synthesis. Identifying <i>DGAT</i> genes in <i>Lindera glauca</i> is essential for studying lipid metabolism pathways and developing novel oil crops with enhanced value. In the study reported in this paper, 15 <i>LgDGAT</i> family genes were first obtained from the <i>L. glauca</i> genome via bioinformatics analysis. We comprehensively analyzed their chromosome distribution, gene structure, subcellular localization, promoter prediction, phylogenetic relationships, tissue-specific expression, and expression patterns during different stages of fruit development. Our findings revealed that LgDGATs can be classified into DGAT1, DGAT2, DGAT3, and WSD (wax ester synthase/acyl-CoA: diacylglycerol acyltransferase) subfamilies distributed across chromosome 3, 5, 6, 8 and 11. <i>LgDGATs</i>’ promoter region showed abundant elements linked to the light response and plant hormone response. Forms of <i>LgDGAT1</i>, <i>LgDGAT2</i>, and <i>LgDGAT3</i> were primarily expressed in the early and late phases of fruit development, indicating their potential function in the growth and development of <i>L. glauca</i>, particularly in oil accumulation. Conversely, <i>LgWSDs</i> exhibited predominant expression in stems and leaves. This paper elucidates the gene structure and expression patterns of <i>LgDGATs</i>, providing a theoretical foundation for understanding the functionality of <i>DGAT</i> genes in <i>Lindera</i> species.
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