The role of miR-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer
Background: MicroRNA (miRNA) regulates gene expression posttranscriptionally, and some of them function in tumor suppression and can be used in drug development. As a result, identifying and screening miRNAs that suppress tumors would be a significant addition to tumor treatment. Methods: In this st...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Computational and Structural Biotechnology Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2001037022003014 |
| _version_ | 1797978207700385792 |
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| author | Lu Qi Lu Wang Fuyao Song Zhenhua Ding Ying Zhang |
| author_facet | Lu Qi Lu Wang Fuyao Song Zhenhua Ding Ying Zhang |
| author_sort | Lu Qi |
| collection | DOAJ |
| description | Background: MicroRNA (miRNA) regulates gene expression posttranscriptionally, and some of them function in tumor suppression and can be used in drug development. As a result, identifying and screening miRNAs that suppress tumors would be a significant addition to tumor treatment. Methods: In this study, we analyzed the miRNA expression profile of colorectal cancer (CRC), constructed a negative regulatory network of the miRNA-target genes, and identified miR-4469 as one of the key tumor suppressors miRNAs. We analyzed the expression and survival of miR-4469 in pan-cancer, experimentally verified the expression level of miR-4469 in CRC cells and the effect on CRC cell proliferation and migration. We screened miR-4469 target genes for enrichment analysis and immune cell infiltration analysis and validated target gene expression to clarify the regulatory mechanisms involved in miR-4469. Results: miR-4469 was more highly expressed in normal colorectum tissues compared to CRC tissues and correlated with survival time in patients with multiple cancers. It was shown that miR-4469 was highly expressed in normal colon cells and miR-4469 expression could inhibit the proliferation and migration of CRC cells. In addition, studies on the mechanism showed that miR-4469 function is mainly related to the regulation of inflammatory cell infiltration, and the key target genes of miR-4469 in this process are SLC2A3, FGR, PLEKHO2, and MYO1F. Conclusion: miR-4469 is a tumor suppressor in CRC, and its regulatory mechanism mainly affects the infiltration of inflammatory cells in the cancer tissue. |
| first_indexed | 2024-04-11T05:19:20Z |
| format | Article |
| id | doaj.art-3ac78546d55c4c12a8b627449c6cfd7f |
| institution | Directory Open Access Journal |
| issn | 2001-0370 |
| language | English |
| last_indexed | 2024-04-11T05:19:20Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Computational and Structural Biotechnology Journal |
| spelling | doaj.art-3ac78546d55c4c12a8b627449c6cfd7f2022-12-24T04:53:28ZengElsevierComputational and Structural Biotechnology Journal2001-03702022-01-012037553763The role of miR-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancerLu Qi0Lu Wang1Fuyao Song2Zhenhua Ding3Ying Zhang4Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China; Guangdong Provincial Key Laboratory of Molecular Oncologic Pathology, Guangzhou 510515, China; Corresponding authors at: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China (L. Qi). Department of Radiation Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, China (Z. Ding and Y. Zhang).Department of Radiation Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, China; Guangdong Provincial Key Laboratory of Tropical Disease Research, Guangzhou 510515, ChinaDepartment of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China; Guangdong Provincial Key Laboratory of Molecular Oncologic Pathology, Guangzhou 510515, ChinaDepartment of Radiation Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, China; Guangdong Provincial Key Laboratory of Tropical Disease Research, Guangzhou 510515, China; Corresponding authors at: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China (L. Qi). Department of Radiation Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, China (Z. Ding and Y. Zhang).Department of Radiation Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, China; Guangdong Provincial Key Laboratory of Tropical Disease Research, Guangzhou 510515, China; Corresponding authors at: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China (L. Qi). Department of Radiation Medicine, School of Public Health, Southern Medical University, Guangzhou 510515, China (Z. Ding and Y. Zhang).Background: MicroRNA (miRNA) regulates gene expression posttranscriptionally, and some of them function in tumor suppression and can be used in drug development. As a result, identifying and screening miRNAs that suppress tumors would be a significant addition to tumor treatment. Methods: In this study, we analyzed the miRNA expression profile of colorectal cancer (CRC), constructed a negative regulatory network of the miRNA-target genes, and identified miR-4469 as one of the key tumor suppressors miRNAs. We analyzed the expression and survival of miR-4469 in pan-cancer, experimentally verified the expression level of miR-4469 in CRC cells and the effect on CRC cell proliferation and migration. We screened miR-4469 target genes for enrichment analysis and immune cell infiltration analysis and validated target gene expression to clarify the regulatory mechanisms involved in miR-4469. Results: miR-4469 was more highly expressed in normal colorectum tissues compared to CRC tissues and correlated with survival time in patients with multiple cancers. It was shown that miR-4469 was highly expressed in normal colon cells and miR-4469 expression could inhibit the proliferation and migration of CRC cells. In addition, studies on the mechanism showed that miR-4469 function is mainly related to the regulation of inflammatory cell infiltration, and the key target genes of miR-4469 in this process are SLC2A3, FGR, PLEKHO2, and MYO1F. Conclusion: miR-4469 is a tumor suppressor in CRC, and its regulatory mechanism mainly affects the infiltration of inflammatory cells in the cancer tissue.http://www.sciencedirect.com/science/article/pii/S2001037022003014miR-4469Colorectal cancerNeutrophilTumor suppressor |
| spellingShingle | Lu Qi Lu Wang Fuyao Song Zhenhua Ding Ying Zhang The role of miR-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer Computational and Structural Biotechnology Journal miR-4469 Colorectal cancer Neutrophil Tumor suppressor |
| title | The role of miR-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer |
| title_full | The role of miR-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer |
| title_fullStr | The role of miR-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer |
| title_full_unstemmed | The role of miR-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer |
| title_short | The role of miR-4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer |
| title_sort | role of mir 4469 as a tumor suppressor regulating inflammatory cell infiltration in colorectal cancer |
| topic | miR-4469 Colorectal cancer Neutrophil Tumor suppressor |
| url | http://www.sciencedirect.com/science/article/pii/S2001037022003014 |
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