Protective effects of Echium amoenum Fisch. and C.A. Mey. against cerebral ischemia in the rats
Background: This study was designed to evaluate the protective effect of Echium amoenum total anthocyanin extract (ETAE) on partial/transient cerebral ischemia in the rats. Materials and Methods: Rats received ETAE (50, 100 and 200 mg/kg, i.p.) 30 min before the induction of cerebral ischemia. Cere...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2015-01-01
|
| Series: | Advanced Biomedical Research |
| Subjects: | |
| Online Access: | http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=107;epage=107;aulast=Safaeian |
| _version_ | 1819064995181232128 |
|---|---|
| author | Leila Safaeian Abolfazl Azami Tameh Alireza Ghannadi Elmira Akbari Naghani Hamed Tavazoei Samaneh Sadat Alavi |
| author_facet | Leila Safaeian Abolfazl Azami Tameh Alireza Ghannadi Elmira Akbari Naghani Hamed Tavazoei Samaneh Sadat Alavi |
| author_sort | Leila Safaeian |
| collection | DOAJ |
| description | Background: This study was designed to evaluate the protective effect of Echium amoenum total anthocyanin extract (ETAE) on partial/transient cerebral ischemia in the rats.
Materials and Methods: Rats received ETAE (50, 100 and 200 mg/kg, i.p.) 30 min before the induction of cerebral ischemia. Cerebral ischemia was induced by bilateral common carotid arteries occlusion (BCCAO) for 30 min, followed by 72 h reperfusion. The neurological deficit, brain performance, and sensory motor function were assessed 48 h and 72 h after surgery. After sacrification, the brains were evaluated for myeloperoxidase (MPO) activity and histopathological changes.
Results: Our results showed that motor function significantly decreased in ischemia/reperfusion (I/R) group as compared to the sham group. Histopathological analysis exhibited the shrinkage and atrophy of the neurons in I/R group. ETAE at the dose of 200 mg/kg improved spontaneous activity and memory induced by cerebral ischemia compared to the control group and also decreased brain MPO activity following cerebral ischemia. However, it could not affect the ability to climbing, body proprioception, vibrissae touch and brain water content. In addition, pretreatment with ETAE at higher doses significantly reduced ischemia-induced neuronal loss of the brain.
Conclusion: The anthocyanin rich fraction from E. amoenum was found to have protective effects against some brain damages postischemic reperfusion . However, further researches are required for investigating the exact mechanisms of the effect of this plant in the prevention of cerebral ischemia in human. |
| first_indexed | 2024-12-21T15:39:25Z |
| format | Article |
| id | doaj.art-3ac8b8ee88bb44058c34d5b549221361 |
| institution | Directory Open Access Journal |
| issn | 2277-9175 2277-9175 |
| language | English |
| last_indexed | 2024-12-21T15:39:25Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Advanced Biomedical Research |
| spelling | doaj.art-3ac8b8ee88bb44058c34d5b5492213612022-12-21T18:58:33ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752277-91752015-01-014110710710.4103/2277-9175.157809Protective effects of Echium amoenum Fisch. and C.A. Mey. against cerebral ischemia in the ratsLeila SafaeianAbolfazl Azami TamehAlireza GhannadiElmira Akbari NaghaniHamed TavazoeiSamaneh Sadat AlaviBackground: This study was designed to evaluate the protective effect of Echium amoenum total anthocyanin extract (ETAE) on partial/transient cerebral ischemia in the rats. Materials and Methods: Rats received ETAE (50, 100 and 200 mg/kg, i.p.) 30 min before the induction of cerebral ischemia. Cerebral ischemia was induced by bilateral common carotid arteries occlusion (BCCAO) for 30 min, followed by 72 h reperfusion. The neurological deficit, brain performance, and sensory motor function were assessed 48 h and 72 h after surgery. After sacrification, the brains were evaluated for myeloperoxidase (MPO) activity and histopathological changes. Results: Our results showed that motor function significantly decreased in ischemia/reperfusion (I/R) group as compared to the sham group. Histopathological analysis exhibited the shrinkage and atrophy of the neurons in I/R group. ETAE at the dose of 200 mg/kg improved spontaneous activity and memory induced by cerebral ischemia compared to the control group and also decreased brain MPO activity following cerebral ischemia. However, it could not affect the ability to climbing, body proprioception, vibrissae touch and brain water content. In addition, pretreatment with ETAE at higher doses significantly reduced ischemia-induced neuronal loss of the brain. Conclusion: The anthocyanin rich fraction from E. amoenum was found to have protective effects against some brain damages postischemic reperfusion . However, further researches are required for investigating the exact mechanisms of the effect of this plant in the prevention of cerebral ischemia in human.http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=107;epage=107;aulast=SafaeianAnthocyaninscerebral ischemiaEchium amoenumrat |
| spellingShingle | Leila Safaeian Abolfazl Azami Tameh Alireza Ghannadi Elmira Akbari Naghani Hamed Tavazoei Samaneh Sadat Alavi Protective effects of Echium amoenum Fisch. and C.A. Mey. against cerebral ischemia in the rats Advanced Biomedical Research Anthocyanins cerebral ischemia Echium amoenum rat |
| title | Protective effects of Echium amoenum Fisch. and C.A. Mey. against cerebral ischemia in the rats |
| title_full | Protective effects of Echium amoenum Fisch. and C.A. Mey. against cerebral ischemia in the rats |
| title_fullStr | Protective effects of Echium amoenum Fisch. and C.A. Mey. against cerebral ischemia in the rats |
| title_full_unstemmed | Protective effects of Echium amoenum Fisch. and C.A. Mey. against cerebral ischemia in the rats |
| title_short | Protective effects of Echium amoenum Fisch. and C.A. Mey. against cerebral ischemia in the rats |
| title_sort | protective effects of echium amoenum fisch and c a mey against cerebral ischemia in the rats |
| topic | Anthocyanins cerebral ischemia Echium amoenum rat |
| url | http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=107;epage=107;aulast=Safaeian |
| work_keys_str_mv | AT leilasafaeian protectiveeffectsofechiumamoenumfischandcameyagainstcerebralischemiaintherats AT abolfazlazamitameh protectiveeffectsofechiumamoenumfischandcameyagainstcerebralischemiaintherats AT alirezaghannadi protectiveeffectsofechiumamoenumfischandcameyagainstcerebralischemiaintherats AT elmiraakbarinaghani protectiveeffectsofechiumamoenumfischandcameyagainstcerebralischemiaintherats AT hamedtavazoei protectiveeffectsofechiumamoenumfischandcameyagainstcerebralischemiaintherats AT samanehsadatalavi protectiveeffectsofechiumamoenumfischandcameyagainstcerebralischemiaintherats |