Oropharyngeal and Sputum Microbiomes Are Similar Following Exacerbation of Chronic Obstructive Pulmonary Disease
Growing evidence suggests that the airway microbiota might be involved in acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Understanding this relationship requires examination of a large-scale population for a long duration to accurately monitor changes in the microbiome. This t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01163/full |
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| author | Hai-Yue Liu Hai-Yue Liu Shi-Yu Zhang Wan-Ying Yang Xiao-Fang Su Yan He Hong-Wei Zhou Jin Su Jin Su |
| author_facet | Hai-Yue Liu Hai-Yue Liu Shi-Yu Zhang Wan-Ying Yang Xiao-Fang Su Yan He Hong-Wei Zhou Jin Su Jin Su |
| author_sort | Hai-Yue Liu |
| collection | DOAJ |
| description | Growing evidence suggests that the airway microbiota might be involved in acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Understanding this relationship requires examination of a large-scale population for a long duration to accurately monitor changes in the microbiome. This type of longitudinal study requires an appropriate sampling strategy; two options are the collection of sputum or oropharyngeal swabs. Comparative analysis of the changes that occur in these two specimen types has not been previously performed. This observational study was conducted to explore oropharyngeal microbial community dynamics over time and to examine the relationship between oropharyngeal swabs and sputum. A total of 114 samples were collected from four patients suffering from severe AECOPD. Bacterial and fungal communities were evaluated using 16S rRNA and ITS sequencing. Inter-individual differences were found in bacterial community structure, but the core genera were shared by both sample types and included 32 lineages. Most of the core genera were members of the phyla Proteobacteria, Firmicutes and Ascomycota. Although the oropharyngeal samples showed higher bacterial alpha diversity, the two sample types generated rather similar taxonomic profiles. These results suggest that the sputum microbiome is remarkably similar to the oropharyngeal microbiome. Thus, oropharyngeal swabs can potentially be used instead of sputum samples for patients with exacerbation of COPD. |
| first_indexed | 2024-12-10T22:35:58Z |
| format | Article |
| id | doaj.art-3ac9419848d34a86acf4e54deef28b1b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T22:35:58Z |
| publishDate | 2017-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-3ac9419848d34a86acf4e54deef28b1b2022-12-22T01:30:53ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-06-01810.3389/fmicb.2017.01163273465Oropharyngeal and Sputum Microbiomes Are Similar Following Exacerbation of Chronic Obstructive Pulmonary DiseaseHai-Yue Liu0Hai-Yue Liu1Shi-Yu Zhang2Wan-Ying Yang3Xiao-Fang Su4Yan He5Hong-Wei Zhou6Jin Su7Jin Su8Department of Environmental Health, School of Public Health, Southern Medical UniversityGuangzhou, ChinaState Key Laboratory of Organ Failure Research, Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical UniversityGuangzhou, ChinaDepartment of Environmental Health, School of Public Health, Southern Medical UniversityGuangzhou, ChinaDepartment of Environmental Health, School of Public Health, Southern Medical UniversityGuangzhou, ChinaChronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical UniversityGuangzhou, ChinaState Key Laboratory of Organ Failure Research, Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical UniversityGuangzhou, ChinaState Key Laboratory of Organ Failure Research, Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical UniversityGuangzhou, ChinaDepartment of Environmental Health, School of Public Health, Southern Medical UniversityGuangzhou, ChinaChronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical UniversityGuangzhou, ChinaGrowing evidence suggests that the airway microbiota might be involved in acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Understanding this relationship requires examination of a large-scale population for a long duration to accurately monitor changes in the microbiome. This type of longitudinal study requires an appropriate sampling strategy; two options are the collection of sputum or oropharyngeal swabs. Comparative analysis of the changes that occur in these two specimen types has not been previously performed. This observational study was conducted to explore oropharyngeal microbial community dynamics over time and to examine the relationship between oropharyngeal swabs and sputum. A total of 114 samples were collected from four patients suffering from severe AECOPD. Bacterial and fungal communities were evaluated using 16S rRNA and ITS sequencing. Inter-individual differences were found in bacterial community structure, but the core genera were shared by both sample types and included 32 lineages. Most of the core genera were members of the phyla Proteobacteria, Firmicutes and Ascomycota. Although the oropharyngeal samples showed higher bacterial alpha diversity, the two sample types generated rather similar taxonomic profiles. These results suggest that the sputum microbiome is remarkably similar to the oropharyngeal microbiome. Thus, oropharyngeal swabs can potentially be used instead of sputum samples for patients with exacerbation of COPD.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01163/fullmicrobiome16S rRNAITSAECOPDsputumoropharyngeal swab |
| spellingShingle | Hai-Yue Liu Hai-Yue Liu Shi-Yu Zhang Wan-Ying Yang Xiao-Fang Su Yan He Hong-Wei Zhou Jin Su Jin Su Oropharyngeal and Sputum Microbiomes Are Similar Following Exacerbation of Chronic Obstructive Pulmonary Disease Frontiers in Microbiology microbiome 16S rRNA ITS AECOPD sputum oropharyngeal swab |
| title | Oropharyngeal and Sputum Microbiomes Are Similar Following Exacerbation of Chronic Obstructive Pulmonary Disease |
| title_full | Oropharyngeal and Sputum Microbiomes Are Similar Following Exacerbation of Chronic Obstructive Pulmonary Disease |
| title_fullStr | Oropharyngeal and Sputum Microbiomes Are Similar Following Exacerbation of Chronic Obstructive Pulmonary Disease |
| title_full_unstemmed | Oropharyngeal and Sputum Microbiomes Are Similar Following Exacerbation of Chronic Obstructive Pulmonary Disease |
| title_short | Oropharyngeal and Sputum Microbiomes Are Similar Following Exacerbation of Chronic Obstructive Pulmonary Disease |
| title_sort | oropharyngeal and sputum microbiomes are similar following exacerbation of chronic obstructive pulmonary disease |
| topic | microbiome 16S rRNA ITS AECOPD sputum oropharyngeal swab |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01163/full |
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