Agaricus blazei as a Substrate for the Production of β-1,3-Glucanase by Trichoderma harzianum Rifai
Extracellular β-1,3-glucanase was produced by Trichoderma harzianum Rifai cultivated in the Agaricus blazei (Agaricus brasiliensis) extract as a substrate in submerged fermentation. A 2²-central composite factorial design was developed using the time of culture (x1/day) and Agaricus blazei extract c...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2011-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/105769 |
| _version_ | 1797426709103902720 |
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| author | Roberto Da Silva Eleni Gomes Aneli M. Barbosa Ellen C. Giese Andréia A. J. Carneiro |
| author_facet | Roberto Da Silva Eleni Gomes Aneli M. Barbosa Ellen C. Giese Andréia A. J. Carneiro |
| author_sort | Roberto Da Silva |
| collection | DOAJ |
| description | Extracellular β-1,3-glucanase was produced by Trichoderma harzianum Rifai cultivated in the Agaricus blazei (Agaricus brasiliensis) extract as a substrate in submerged fermentation. A 2²-central composite factorial design was developed using the time of culture (x1/day) and Agaricus blazei extract concentration (x2/(g/L)) as variables, and the results were analyzed using response surface methodology (RSM). The results showed that the Agaricus blazei extract concentration was the most important variable in the production of β-1,3-glucanase, and the maximum β-1,3-glucanase activity (0.77 U/mL) was obtained in one day of cultivation. The β-glucan present in the cell wall of Agaricus blazei mushroom proved to be a good substrate for inducing the production of specific β-1,3-glucanase by Trichoderma harzianum Rifai. |
| first_indexed | 2024-03-09T08:33:42Z |
| format | Article |
| id | doaj.art-3acab321c5604f9692848ee8cf99d0c4 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T08:33:42Z |
| publishDate | 2011-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-3acab321c5604f9692848ee8cf99d0c42023-12-02T18:57:31ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062011-01-01493341346Agaricus blazei as a Substrate for the Production of β-1,3-Glucanase by Trichoderma harzianum RifaiRoberto Da Silva0Eleni Gomes1Aneli M. Barbosa2Ellen C. Giese3Andréia A. J. Carneiro4São Paulo State University, UNESP, IBILCE, Laboratory of Biochemistry and Applied Microbiology, R. Cristóvão Colombo 2265, 15054-000 São José do Rio Preto-SP, BrazilSão Paulo State University, UNESP, IBILCE, Laboratory of Biochemistry and Applied Microbiology, R. Cristóvão Colombo 2265, 15054-000 São José do Rio Preto-SP, BrazilState University of Londrina, Department of Biochemistry and Biotechnology, Rod. Celso Garcia Cid s/n, 86051-990 Londrina-PR, BrazilBiorefining Research Initiative, Lakehead University, P7B5E1 Thunder Bay, Ontario, CanadaSão Paulo State University, UNESP, IBILCE, Laboratory of Biochemistry and Applied Microbiology, R. Cristóvão Colombo 2265, 15054-000 São José do Rio Preto-SP, BrazilExtracellular β-1,3-glucanase was produced by Trichoderma harzianum Rifai cultivated in the Agaricus blazei (Agaricus brasiliensis) extract as a substrate in submerged fermentation. A 2²-central composite factorial design was developed using the time of culture (x1/day) and Agaricus blazei extract concentration (x2/(g/L)) as variables, and the results were analyzed using response surface methodology (RSM). The results showed that the Agaricus blazei extract concentration was the most important variable in the production of β-1,3-glucanase, and the maximum β-1,3-glucanase activity (0.77 U/mL) was obtained in one day of cultivation. The β-glucan present in the cell wall of Agaricus blazei mushroom proved to be a good substrate for inducing the production of specific β-1,3-glucanase by Trichoderma harzianum Rifai.http://hrcak.srce.hr/file/105769β-1,3-glucanasemedicinal mushroomsTrichoderma harzianum Rifairesponse surface methodology |
| spellingShingle | Roberto Da Silva Eleni Gomes Aneli M. Barbosa Ellen C. Giese Andréia A. J. Carneiro Agaricus blazei as a Substrate for the Production of β-1,3-Glucanase by Trichoderma harzianum Rifai Food Technology and Biotechnology β-1,3-glucanase medicinal mushrooms Trichoderma harzianum Rifai response surface methodology |
| title | Agaricus blazei as a Substrate for the Production of β-1,3-Glucanase by Trichoderma harzianum Rifai |
| title_full | Agaricus blazei as a Substrate for the Production of β-1,3-Glucanase by Trichoderma harzianum Rifai |
| title_fullStr | Agaricus blazei as a Substrate for the Production of β-1,3-Glucanase by Trichoderma harzianum Rifai |
| title_full_unstemmed | Agaricus blazei as a Substrate for the Production of β-1,3-Glucanase by Trichoderma harzianum Rifai |
| title_short | Agaricus blazei as a Substrate for the Production of β-1,3-Glucanase by Trichoderma harzianum Rifai |
| title_sort | agaricus blazei as a substrate for the production of β 1 3 glucanase by trichoderma harzianum rifai |
| topic | β-1,3-glucanase medicinal mushrooms Trichoderma harzianum Rifai response surface methodology |
| url | http://hrcak.srce.hr/file/105769 |
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