Virus-Induced Gene Silencing of <i>SlWRKY79</i> Attenuates Salt Tolerance in Tomato Plants

Previous studies have shown that WRKY transcription factors play important roles in abiotic stress responses. Thus, virus-induced gene silencing (VIGS) was used to identify the function of <i>SlWRKY79</i> in the salt tolerance of tomato plants by downregulating the expression of the <...

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Bibliographic Details
Main Authors: Yuqing He, Xiaochun Zhang, Yinxiao Tan, Deli Si, Tingting Zhao, Xiangyang Xu, Jingbin Jiang, Huanhuan Yang, Jingfu Li
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Agronomy
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Online Access:https://www.mdpi.com/2073-4395/11/8/1519
Description
Summary:Previous studies have shown that WRKY transcription factors play important roles in abiotic stress responses. Thus, virus-induced gene silencing (VIGS) was used to identify the function of <i>SlWRKY79</i> in the salt tolerance of tomato plants by downregulating the expression of the <i>SlWRKY79</i> gene. Under the same salt treatment conditions, the <i>SlWRKY79</i>-silenced plants showed faster stem wilting and more severe leaf shrinkage than the control plants, and the bending degree of the stem of the <i>SlWRKY79</i>-silenced plants was also greater than that of the control plants. Physiological analyses showed that considerably higher levels of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), superoxide anion (O<sup>2−</sup>), and abscisic acid (ABA) accumulated in the leaves of the <i>SlWRKY79</i>-silenced plants than in those of the controls after salt treatment. Taken together, our results suggested that <i>SlWRKY79</i> plays a positive regulatory role in salt tolerance in tomato plants.
ISSN:2073-4395