Virus-Induced Gene Silencing of <i>SlWRKY79</i> Attenuates Salt Tolerance in Tomato Plants
Previous studies have shown that WRKY transcription factors play important roles in abiotic stress responses. Thus, virus-induced gene silencing (VIGS) was used to identify the function of <i>SlWRKY79</i> in the salt tolerance of tomato plants by downregulating the expression of the <...
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MDPI AG
2021-07-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/8/1519 |
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| author | Yuqing He Xiaochun Zhang Yinxiao Tan Deli Si Tingting Zhao Xiangyang Xu Jingbin Jiang Huanhuan Yang Jingfu Li |
| author_facet | Yuqing He Xiaochun Zhang Yinxiao Tan Deli Si Tingting Zhao Xiangyang Xu Jingbin Jiang Huanhuan Yang Jingfu Li |
| author_sort | Yuqing He |
| collection | DOAJ |
| description | Previous studies have shown that WRKY transcription factors play important roles in abiotic stress responses. Thus, virus-induced gene silencing (VIGS) was used to identify the function of <i>SlWRKY79</i> in the salt tolerance of tomato plants by downregulating the expression of the <i>SlWRKY79</i> gene. Under the same salt treatment conditions, the <i>SlWRKY79</i>-silenced plants showed faster stem wilting and more severe leaf shrinkage than the control plants, and the bending degree of the stem of the <i>SlWRKY79</i>-silenced plants was also greater than that of the control plants. Physiological analyses showed that considerably higher levels of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), superoxide anion (O<sup>2−</sup>), and abscisic acid (ABA) accumulated in the leaves of the <i>SlWRKY79</i>-silenced plants than in those of the controls after salt treatment. Taken together, our results suggested that <i>SlWRKY79</i> plays a positive regulatory role in salt tolerance in tomato plants. |
| first_indexed | 2024-03-10T09:05:07Z |
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| id | doaj.art-3acb9e69654e40a2870984a88ed503f5 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T09:05:07Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-3acb9e69654e40a2870984a88ed503f52023-11-22T06:25:00ZengMDPI AGAgronomy2073-43952021-07-01118151910.3390/agronomy11081519Virus-Induced Gene Silencing of <i>SlWRKY79</i> Attenuates Salt Tolerance in Tomato PlantsYuqing He0Xiaochun Zhang1Yinxiao Tan2Deli Si3Tingting Zhao4Xiangyang Xu5Jingbin Jiang6Huanhuan Yang7Jingfu Li8School of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaSchool of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaSchool of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaSchool of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaSchool of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaSchool of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaSchool of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaSchool of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaSchool of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaPrevious studies have shown that WRKY transcription factors play important roles in abiotic stress responses. Thus, virus-induced gene silencing (VIGS) was used to identify the function of <i>SlWRKY79</i> in the salt tolerance of tomato plants by downregulating the expression of the <i>SlWRKY79</i> gene. Under the same salt treatment conditions, the <i>SlWRKY79</i>-silenced plants showed faster stem wilting and more severe leaf shrinkage than the control plants, and the bending degree of the stem of the <i>SlWRKY79</i>-silenced plants was also greater than that of the control plants. Physiological analyses showed that considerably higher levels of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), superoxide anion (O<sup>2−</sup>), and abscisic acid (ABA) accumulated in the leaves of the <i>SlWRKY79</i>-silenced plants than in those of the controls after salt treatment. Taken together, our results suggested that <i>SlWRKY79</i> plays a positive regulatory role in salt tolerance in tomato plants.https://www.mdpi.com/2073-4395/11/8/1519<i>SlWRKY79</i>salt resistanceVIGStomato |
| spellingShingle | Yuqing He Xiaochun Zhang Yinxiao Tan Deli Si Tingting Zhao Xiangyang Xu Jingbin Jiang Huanhuan Yang Jingfu Li Virus-Induced Gene Silencing of <i>SlWRKY79</i> Attenuates Salt Tolerance in Tomato Plants Agronomy <i>SlWRKY79</i> salt resistance VIGS tomato |
| title | Virus-Induced Gene Silencing of <i>SlWRKY79</i> Attenuates Salt Tolerance in Tomato Plants |
| title_full | Virus-Induced Gene Silencing of <i>SlWRKY79</i> Attenuates Salt Tolerance in Tomato Plants |
| title_fullStr | Virus-Induced Gene Silencing of <i>SlWRKY79</i> Attenuates Salt Tolerance in Tomato Plants |
| title_full_unstemmed | Virus-Induced Gene Silencing of <i>SlWRKY79</i> Attenuates Salt Tolerance in Tomato Plants |
| title_short | Virus-Induced Gene Silencing of <i>SlWRKY79</i> Attenuates Salt Tolerance in Tomato Plants |
| title_sort | virus induced gene silencing of i slwrky79 i attenuates salt tolerance in tomato plants |
| topic | <i>SlWRKY79</i> salt resistance VIGS tomato |
| url | https://www.mdpi.com/2073-4395/11/8/1519 |
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