A tail of two phages: Genomic and functional analysis of Listeria monocytogenes phages vB_LmoS_188 and vB_LmoS_293 reveal the receptor-binding proteins involved in host specificity.
The physical characteristics of bacteriophages establish them as viable candidates for downstream development of pathogen detection assays and biocontrol measures. To utilize phages for such purposes, a detailed knowledge of their host interaction mechanisms is a prerequisite. There is currently a w...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01107/full |
| _version_ | 1811197464986779648 |
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| author | Aidan eCasey Aidan eCasey Kieran eJordan Horst eNeve Aidan eCoffey Olivia eMcAuliffe |
| author_facet | Aidan eCasey Aidan eCasey Kieran eJordan Horst eNeve Aidan eCoffey Olivia eMcAuliffe |
| author_sort | Aidan eCasey |
| collection | DOAJ |
| description | The physical characteristics of bacteriophages establish them as viable candidates for downstream development of pathogen detection assays and biocontrol measures. To utilize phages for such purposes, a detailed knowledge of their host interaction mechanisms is a prerequisite. There is currently a wealth of knowledge available concerning Gram-negative phage-host interaction, but little by comparison for Gram-positive phages and Listeria phages in particular. In this research, the lytic spectrum of two recently isolated Listeria monocytogenes phages (vB_LmoS_188 and vB_LmoS_293) was determined, and the genomic basis for their observed serotype 4b/4e host-specificity was investigated using comparative genomics. The late tail genes of these phages were identified to be highly conserved when compared to other serovar 4-specific Listeria phages. Spontaneous mutants of each of these phages with broadened host specificities were generated. Their late tail gene sequences were compared with their wild-type counterparts resulting in the putative identification of the products of ORF 19 of vB_LmoS_188 and ORF 20 of vB_LmoS_293 as the receptor binding proteins of these phages. The research findings also indicate that conserved baseplate architectures and host interaction mechanisms exist for Listeria siphoviruses with differing host-specificities, and further contribute to the current knowledge of phage-host interactions with regard to Listeria phages. |
| first_indexed | 2024-04-12T01:14:12Z |
| format | Article |
| id | doaj.art-3acf9104ce2d4911a0411c870fa901dd |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T01:14:12Z |
| publishDate | 2015-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-3acf9104ce2d4911a0411c870fa901dd2022-12-22T03:54:00ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-10-01610.3389/fmicb.2015.01107163279A tail of two phages: Genomic and functional analysis of Listeria monocytogenes phages vB_LmoS_188 and vB_LmoS_293 reveal the receptor-binding proteins involved in host specificity.Aidan eCasey0Aidan eCasey1Kieran eJordan2Horst eNeve3Aidan eCoffey4Olivia eMcAuliffe5TeagascCork Institute of TechnologyTeagascMax-Rubner Institut - Federal Research Institute of Nutrition and FoodCork Institute of TechnologyTeagascThe physical characteristics of bacteriophages establish them as viable candidates for downstream development of pathogen detection assays and biocontrol measures. To utilize phages for such purposes, a detailed knowledge of their host interaction mechanisms is a prerequisite. There is currently a wealth of knowledge available concerning Gram-negative phage-host interaction, but little by comparison for Gram-positive phages and Listeria phages in particular. In this research, the lytic spectrum of two recently isolated Listeria monocytogenes phages (vB_LmoS_188 and vB_LmoS_293) was determined, and the genomic basis for their observed serotype 4b/4e host-specificity was investigated using comparative genomics. The late tail genes of these phages were identified to be highly conserved when compared to other serovar 4-specific Listeria phages. Spontaneous mutants of each of these phages with broadened host specificities were generated. Their late tail gene sequences were compared with their wild-type counterparts resulting in the putative identification of the products of ORF 19 of vB_LmoS_188 and ORF 20 of vB_LmoS_293 as the receptor binding proteins of these phages. The research findings also indicate that conserved baseplate architectures and host interaction mechanisms exist for Listeria siphoviruses with differing host-specificities, and further contribute to the current knowledge of phage-host interactions with regard to Listeria phages.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01107/fullBacteriophagesListeria monocytogenesSiphoviridaeserotypeReceptor binding protein |
| spellingShingle | Aidan eCasey Aidan eCasey Kieran eJordan Horst eNeve Aidan eCoffey Olivia eMcAuliffe A tail of two phages: Genomic and functional analysis of Listeria monocytogenes phages vB_LmoS_188 and vB_LmoS_293 reveal the receptor-binding proteins involved in host specificity. Frontiers in Microbiology Bacteriophages Listeria monocytogenes Siphoviridae serotype Receptor binding protein |
| title | A tail of two phages: Genomic and functional analysis of Listeria monocytogenes phages vB_LmoS_188 and vB_LmoS_293 reveal the receptor-binding proteins involved in host specificity. |
| title_full | A tail of two phages: Genomic and functional analysis of Listeria monocytogenes phages vB_LmoS_188 and vB_LmoS_293 reveal the receptor-binding proteins involved in host specificity. |
| title_fullStr | A tail of two phages: Genomic and functional analysis of Listeria monocytogenes phages vB_LmoS_188 and vB_LmoS_293 reveal the receptor-binding proteins involved in host specificity. |
| title_full_unstemmed | A tail of two phages: Genomic and functional analysis of Listeria monocytogenes phages vB_LmoS_188 and vB_LmoS_293 reveal the receptor-binding proteins involved in host specificity. |
| title_short | A tail of two phages: Genomic and functional analysis of Listeria monocytogenes phages vB_LmoS_188 and vB_LmoS_293 reveal the receptor-binding proteins involved in host specificity. |
| title_sort | tail of two phages genomic and functional analysis of listeria monocytogenes phages vb lmos 188 and vb lmos 293 reveal the receptor binding proteins involved in host specificity |
| topic | Bacteriophages Listeria monocytogenes Siphoviridae serotype Receptor binding protein |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01107/full |
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