In vitro hemolytic activity of Bothrops lanceolatus (fer-de-lance) venom
Bothrops lanceolatus venom contains a variety of enzymatic and biological activities. The present work investigated the hemolytic activity of this venom and its phospholipase A2 (PLA2). Bothrops lanceolatus venom (6.7 µg/mL) caused indirect hemolysis of cow, horse, rat and sheep erythrocytes, with h...
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| Format: | Article |
| Language: | English |
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SciELO
2009-01-01
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| Series: | Journal of Venomous Animals and Toxins including Tropical Diseases |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300011 |
| _version_ | 1818247707760787456 |
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| author | LJ Martins PMF de Araújo C Bon S Hyslop AL de Araújo |
| author_facet | LJ Martins PMF de Araújo C Bon S Hyslop AL de Araújo |
| author_sort | LJ Martins |
| collection | DOAJ |
| description | Bothrops lanceolatus venom contains a variety of enzymatic and biological activities. The present work investigated the hemolytic activity of this venom and its phospholipase A2 (PLA2). Bothrops lanceolatus venom (6.7 µg/mL) caused indirect hemolysis of cow, horse, rat and sheep erythrocytes, with horse erythrocytes being the most sensitive; no direct hemolysis was observed. Hemolysis in sheep erythrocytes was concentration-dependent (5-11.7 µg/mL) and markedly attenuated by heating the venom for 30 minutes at ≥ 40°C and by the PLA2 inhibitor p-bromophenacyl bromide. An acidic PLA2 (5 µg/mL) purified from B. lanceolatus venom also caused hemolysis. This PLA2 showed immunoprecipitin lines with antivenom against B. lanceolatus, which suggests that the enzymatic and hemolytic activities of this enzyme may be neutralized during antivenom therapy. These results indicate that B. lanceolatus venom and its PLA2 can cause hemolysis in vitro. |
| first_indexed | 2024-12-12T15:08:59Z |
| format | Article |
| id | doaj.art-3ad287494462433e80529f78f7553369 |
| institution | Directory Open Access Journal |
| issn | 1678-9199 |
| language | English |
| last_indexed | 2024-12-12T15:08:59Z |
| publishDate | 2009-01-01 |
| publisher | SciELO |
| record_format | Article |
| series | Journal of Venomous Animals and Toxins including Tropical Diseases |
| spelling | doaj.art-3ad287494462433e80529f78f75533692022-12-22T00:20:40ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992009-01-0115349850810.1590/S1678-91992009000300011In vitro hemolytic activity of Bothrops lanceolatus (fer-de-lance) venomLJ MartinsPMF de AraújoC BonS HyslopAL de AraújoBothrops lanceolatus venom contains a variety of enzymatic and biological activities. The present work investigated the hemolytic activity of this venom and its phospholipase A2 (PLA2). Bothrops lanceolatus venom (6.7 µg/mL) caused indirect hemolysis of cow, horse, rat and sheep erythrocytes, with horse erythrocytes being the most sensitive; no direct hemolysis was observed. Hemolysis in sheep erythrocytes was concentration-dependent (5-11.7 µg/mL) and markedly attenuated by heating the venom for 30 minutes at ≥ 40°C and by the PLA2 inhibitor p-bromophenacyl bromide. An acidic PLA2 (5 µg/mL) purified from B. lanceolatus venom also caused hemolysis. This PLA2 showed immunoprecipitin lines with antivenom against B. lanceolatus, which suggests that the enzymatic and hemolytic activities of this enzyme may be neutralized during antivenom therapy. These results indicate that B. lanceolatus venom and its PLA2 can cause hemolysis in vitro.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300011Bothrops lanceolatushemolytic activityphospholipase A2snake venom |
| spellingShingle | LJ Martins PMF de Araújo C Bon S Hyslop AL de Araújo In vitro hemolytic activity of Bothrops lanceolatus (fer-de-lance) venom Journal of Venomous Animals and Toxins including Tropical Diseases Bothrops lanceolatus hemolytic activity phospholipase A2 snake venom |
| title | In vitro hemolytic activity of Bothrops lanceolatus (fer-de-lance) venom |
| title_full | In vitro hemolytic activity of Bothrops lanceolatus (fer-de-lance) venom |
| title_fullStr | In vitro hemolytic activity of Bothrops lanceolatus (fer-de-lance) venom |
| title_full_unstemmed | In vitro hemolytic activity of Bothrops lanceolatus (fer-de-lance) venom |
| title_short | In vitro hemolytic activity of Bothrops lanceolatus (fer-de-lance) venom |
| title_sort | in vitro hemolytic activity of bothrops lanceolatus fer de lance venom |
| topic | Bothrops lanceolatus hemolytic activity phospholipase A2 snake venom |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300011 |
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