Zebrafish as a New Tool in Heart Preservation Research
Heart transplantation became a reality at the end of the 1960s as a life-saving option for patients with end-stage heart failure. Static cold storage (SCS) at 4–6 °C has remained the standard for heart preservation for decades. However, SCS only allows for short-term storage that precludes optimal m...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Journal of Cardiovascular Development and Disease |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2308-3425/8/4/39 |
| _version_ | 1797538460300476416 |
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| author | Luciana Da Silveira Cavalcante Shannon N. Tessier |
| author_facet | Luciana Da Silveira Cavalcante Shannon N. Tessier |
| author_sort | Luciana Da Silveira Cavalcante |
| collection | DOAJ |
| description | Heart transplantation became a reality at the end of the 1960s as a life-saving option for patients with end-stage heart failure. Static cold storage (SCS) at 4–6 °C has remained the standard for heart preservation for decades. However, SCS only allows for short-term storage that precludes optimal matching programs, requires emergency surgeries, and results in the unnecessary discard of organs. Among the alternatives seeking to extend ex vivo lifespan and mitigate the shortage of organs are sub-zero or machine perfusion modalities. Sub-zero approaches aim to prolong cold ischemia tolerance by deepening metabolic stasis, while machine perfusion aims to support metabolism through the continuous delivery of oxygen and nutrients. Each of these approaches hold promise; however, complex barriers must be overcome before their potential can be fully realized. We suggest that one barrier facing all experimental efforts to extend ex vivo lifespan are limited research tools. Mammalian models are usually the first choice due to translational aspects, yet experimentation can be restricted by expertise, time, and resources. Instead, there are instances when smaller vertebrate models, like the zebrafish, could fill critical experimental gaps in the field. Taken together, this review provides a summary of the current gold standard for heart preservation as well as new technologies in ex vivo lifespan extension. Furthermore, we describe how existing tools in zebrafish research, including isolated organ, cell specific and functional assays, as well as molecular tools, could complement and elevate heart preservation research. |
| first_indexed | 2024-03-10T12:30:44Z |
| format | Article |
| id | doaj.art-3ad60c6d46be4d1bb4554fd445c0940c |
| institution | Directory Open Access Journal |
| issn | 2308-3425 |
| language | English |
| last_indexed | 2024-03-10T12:30:44Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Cardiovascular Development and Disease |
| spelling | doaj.art-3ad60c6d46be4d1bb4554fd445c0940c2023-11-21T14:37:32ZengMDPI AGJournal of Cardiovascular Development and Disease2308-34252021-04-01843910.3390/jcdd8040039Zebrafish as a New Tool in Heart Preservation ResearchLuciana Da Silveira Cavalcante0Shannon N. Tessier1Center for Engineering in Medicine and Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA 2114, USACenter for Engineering in Medicine and Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA 2114, USAHeart transplantation became a reality at the end of the 1960s as a life-saving option for patients with end-stage heart failure. Static cold storage (SCS) at 4–6 °C has remained the standard for heart preservation for decades. However, SCS only allows for short-term storage that precludes optimal matching programs, requires emergency surgeries, and results in the unnecessary discard of organs. Among the alternatives seeking to extend ex vivo lifespan and mitigate the shortage of organs are sub-zero or machine perfusion modalities. Sub-zero approaches aim to prolong cold ischemia tolerance by deepening metabolic stasis, while machine perfusion aims to support metabolism through the continuous delivery of oxygen and nutrients. Each of these approaches hold promise; however, complex barriers must be overcome before their potential can be fully realized. We suggest that one barrier facing all experimental efforts to extend ex vivo lifespan are limited research tools. Mammalian models are usually the first choice due to translational aspects, yet experimentation can be restricted by expertise, time, and resources. Instead, there are instances when smaller vertebrate models, like the zebrafish, could fill critical experimental gaps in the field. Taken together, this review provides a summary of the current gold standard for heart preservation as well as new technologies in ex vivo lifespan extension. Furthermore, we describe how existing tools in zebrafish research, including isolated organ, cell specific and functional assays, as well as molecular tools, could complement and elevate heart preservation research.https://www.mdpi.com/2308-3425/8/4/39heart preservationheart transplantationregenerative medicinezebrafish |
| spellingShingle | Luciana Da Silveira Cavalcante Shannon N. Tessier Zebrafish as a New Tool in Heart Preservation Research Journal of Cardiovascular Development and Disease heart preservation heart transplantation regenerative medicine zebrafish |
| title | Zebrafish as a New Tool in Heart Preservation Research |
| title_full | Zebrafish as a New Tool in Heart Preservation Research |
| title_fullStr | Zebrafish as a New Tool in Heart Preservation Research |
| title_full_unstemmed | Zebrafish as a New Tool in Heart Preservation Research |
| title_short | Zebrafish as a New Tool in Heart Preservation Research |
| title_sort | zebrafish as a new tool in heart preservation research |
| topic | heart preservation heart transplantation regenerative medicine zebrafish |
| url | https://www.mdpi.com/2308-3425/8/4/39 |
| work_keys_str_mv | AT lucianadasilveiracavalcante zebrafishasanewtoolinheartpreservationresearch AT shannonntessier zebrafishasanewtoolinheartpreservationresearch |