Fecal PCR survey and genome analysis of Lawsonia intracellularis in China
Proliferative enteropathy caused by Lawsonia intracellularis is an important economic associated disease to pig industry, but the knowledge about the prevalence of L. intracellularis in pig farms in China is limited. In addition, there is no complete genome sequence available for L. intracellularis...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1324768/full |
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| author | Lei Wang Lei Wang Lei Wang Wenqing Wu Wenqing Wu Lifeng Zhao Lifeng Zhao Zhanwei Zhu Zhanwei Zhu Xinzhi Yao Jie Fan Jie Fan Hongjian Chen Hongjian Chen Wenbo Song Wenbo Song Xi Huang Xi Huang Lin Hua Lin Hua Ping Qian Ping Qian Huanchun Chen Huanchun Chen Zhong Peng Zhong Peng Zhong Peng Bin Wu Bin Wu |
| author_facet | Lei Wang Lei Wang Lei Wang Wenqing Wu Wenqing Wu Lifeng Zhao Lifeng Zhao Zhanwei Zhu Zhanwei Zhu Xinzhi Yao Jie Fan Jie Fan Hongjian Chen Hongjian Chen Wenbo Song Wenbo Song Xi Huang Xi Huang Lin Hua Lin Hua Ping Qian Ping Qian Huanchun Chen Huanchun Chen Zhong Peng Zhong Peng Zhong Peng Bin Wu Bin Wu |
| author_sort | Lei Wang |
| collection | DOAJ |
| description | Proliferative enteropathy caused by Lawsonia intracellularis is an important economic associated disease to pig industry, but the knowledge about the prevalence of L. intracellularis in pig farms in China is limited. In addition, there is no complete genome sequence available for L. intracellularis isolates from China. In this study, we developed a TaqMan qPCR for the screening of L. intracellularis by targeting the bacterial 16S rDNA gene. Laboratory evaluations revealed a good sensitivity and specificity on detecting L. intracellularis nucleic acid. Using this method, we investigated 891 fecal samples from apparently healthy pigs in 47 farms. The results demonstrated a screening positive rate of 37.3% (95% CI, 34.1–40.5%) for the samples, and a farm screening positive rate of 93.6% (95% CI, 65.3–94.4%). The screening positive rate at herd level ranged from 6.67% (95% CI, 0.2–31.9%) to 40% (95% CI, 38–79.6%), while at animal level, the highest screening positive rate was found in 12-week-old pigs [85.7% (95% CI, 67.3–96.0%)]. Investigation of 705 diarrheal or bloody feces from symptomatic pigs revealed that the highest positive rate was found in replacement gilts which was 37.18% (95% CI, 45.1–89.5%). Secondly, we conducted the complete genome sequence of a L. intracellularis PPE-GX01-2022 from China through PacBio sequencing. The genome of PPE-GX01-2022 consisted of a chromosome of 1,439,110 bp in length and three plasmids of 193,063, 39,799, and 27,067 bp, respectively. This genome encoded 1,428 predicted proteins, 44 tRNAs, and 6 rRNAs. Sequence comparisons demonstrated that the genome sequence of PPE-GX01-2022 was highly homologous to those of two isolates from US, and these three isolates shared 1,378 core genes. The screening results suggest a high prevalence rate of L. intracellularis in Chinese pig farms. In addition, the genome sequence of the Chinese isolate was highly homologous to those of the field isolates from the US. |
| first_indexed | 2024-03-08T05:13:00Z |
| format | Article |
| id | doaj.art-3ad850b3259b41f3bd8e3dc6bdc7c663 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-08T05:13:00Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-3ad850b3259b41f3bd8e3dc6bdc7c6632024-02-07T05:21:32ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-02-011110.3389/fvets.2024.13247681324768Fecal PCR survey and genome analysis of Lawsonia intracellularis in ChinaLei Wang0Lei Wang1Lei Wang2Wenqing Wu3Wenqing Wu4Lifeng Zhao5Lifeng Zhao6Zhanwei Zhu7Zhanwei Zhu8Xinzhi Yao9Jie Fan10Jie Fan11Hongjian Chen12Hongjian Chen13Wenbo Song14Wenbo Song15Xi Huang16Xi Huang17Lin Hua18Lin Hua19Ping Qian20Ping Qian21Huanchun Chen22Huanchun Chen23Zhong Peng24Zhong Peng25Zhong Peng26Bin Wu27Bin Wu28State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaGuangxi YangXiang Co., Ltd., Guigang, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaCollege of Informatics, Hubei Key Laboratory of Agricultural Bioinformatics, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaHubei Hongshan Laboratory, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, ChinaProliferative enteropathy caused by Lawsonia intracellularis is an important economic associated disease to pig industry, but the knowledge about the prevalence of L. intracellularis in pig farms in China is limited. In addition, there is no complete genome sequence available for L. intracellularis isolates from China. In this study, we developed a TaqMan qPCR for the screening of L. intracellularis by targeting the bacterial 16S rDNA gene. Laboratory evaluations revealed a good sensitivity and specificity on detecting L. intracellularis nucleic acid. Using this method, we investigated 891 fecal samples from apparently healthy pigs in 47 farms. The results demonstrated a screening positive rate of 37.3% (95% CI, 34.1–40.5%) for the samples, and a farm screening positive rate of 93.6% (95% CI, 65.3–94.4%). The screening positive rate at herd level ranged from 6.67% (95% CI, 0.2–31.9%) to 40% (95% CI, 38–79.6%), while at animal level, the highest screening positive rate was found in 12-week-old pigs [85.7% (95% CI, 67.3–96.0%)]. Investigation of 705 diarrheal or bloody feces from symptomatic pigs revealed that the highest positive rate was found in replacement gilts which was 37.18% (95% CI, 45.1–89.5%). Secondly, we conducted the complete genome sequence of a L. intracellularis PPE-GX01-2022 from China through PacBio sequencing. The genome of PPE-GX01-2022 consisted of a chromosome of 1,439,110 bp in length and three plasmids of 193,063, 39,799, and 27,067 bp, respectively. This genome encoded 1,428 predicted proteins, 44 tRNAs, and 6 rRNAs. Sequence comparisons demonstrated that the genome sequence of PPE-GX01-2022 was highly homologous to those of two isolates from US, and these three isolates shared 1,378 core genes. The screening results suggest a high prevalence rate of L. intracellularis in Chinese pig farms. In addition, the genome sequence of the Chinese isolate was highly homologous to those of the field isolates from the US.https://www.frontiersin.org/articles/10.3389/fvets.2024.1324768/fullLawsonia intracellularisTaqMan qPCRscreeningpig farmscomplete genome sequence |
| spellingShingle | Lei Wang Lei Wang Lei Wang Wenqing Wu Wenqing Wu Lifeng Zhao Lifeng Zhao Zhanwei Zhu Zhanwei Zhu Xinzhi Yao Jie Fan Jie Fan Hongjian Chen Hongjian Chen Wenbo Song Wenbo Song Xi Huang Xi Huang Lin Hua Lin Hua Ping Qian Ping Qian Huanchun Chen Huanchun Chen Zhong Peng Zhong Peng Zhong Peng Bin Wu Bin Wu Fecal PCR survey and genome analysis of Lawsonia intracellularis in China Frontiers in Veterinary Science Lawsonia intracellularis TaqMan qPCR screening pig farms complete genome sequence |
| title | Fecal PCR survey and genome analysis of Lawsonia intracellularis in China |
| title_full | Fecal PCR survey and genome analysis of Lawsonia intracellularis in China |
| title_fullStr | Fecal PCR survey and genome analysis of Lawsonia intracellularis in China |
| title_full_unstemmed | Fecal PCR survey and genome analysis of Lawsonia intracellularis in China |
| title_short | Fecal PCR survey and genome analysis of Lawsonia intracellularis in China |
| title_sort | fecal pcr survey and genome analysis of lawsonia intracellularis in china |
| topic | Lawsonia intracellularis TaqMan qPCR screening pig farms complete genome sequence |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1324768/full |
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